Inhibition of the yeast-mycelial transition and the phorogenesis of Mucorales by diamino butanone.

Abstract:

:Diamino butanone (DAB), a competitive inhibitor of ornithine decarboxylase (ODC) a key enzyme in polyamine biosynthesis, inhibited the yeast to hyphae transition in Mucor rouxii, induced by transfer from anaerobiosis to aerobiosis, but not the opposite phenomenon. Addition of DAB to anaerobic yeast cells brought about a decrease in ODC and polyamine levels. In these conditions, the aerobic shift produced only a weak increase in ODC activity and no change in polyamine levels. DAB also blocked phorogenesis in M. rouxii and in Phycomyces blakesleeanus. At the effective concentrations DAB did not affect cell growth of either fungus. It is suggested that low, constant levels of ODC and polyamines are necessary for cell growth, and that high transient levels are required during the differentiative steps. DAB, at the concentrations used, affects this last process, but does not interfere with the maintenance level of polyamines.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Martinez-Pacheco M,Rodriguez G,Reyna G,Calvo-Mendez C,Ruiz-Herrera J

doi

10.1007/BF00444661

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

10-4

issue

1

eissn

0302-8933

issn

1432-072X

journal_volume

151

pub_type

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