Abstract:
:Recently, it was proposed that some antibiotics stimulate the production of reactive oxygen species (ROS), which contribute to cell death. Later, other research groups have provided arguments against ROS-mediated killing of bacteria by antibiotics. At present, there remain a number of unanswered questions in understanding of the role of ROS in killing by antibiotics. Mutants of Escherichia coli in components of the thioredoxin and glutaredoxin redox pathways used in this study possess a great variability in antioxidant activity, and they therefore are a useful model for the investigation of the role of oxidative stress in bactericidal effect of antibiotics. Statistical analysis did not reveal a significant correlation between the susceptibility of the mutants to ciprofloxacin and ampicillin and their resistance to peroxide stress. However, we found strong reverse correlations between the bactericidal activity of antibiotics and the specific growth rate of these mutants at the moment of drug addition. Supplements changing the level of intra- and extracellular glutathione considerably affected E. coli susceptibility to ciprofloxacin and ampicillin. The effect of GSH precursors on bactericidal activity of antibiotics was also observed in gshA mutants.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Smirnova G,Muzyka N,Lepekhina E,Oktyabrsky Odoi
10.1007/s00203-016-1247-zsubject
Has Abstractpub_date
2016-11-01 00:00:00pages
913-21issue
9eissn
0302-8933issn
1432-072Xpii
10.1007/s00203-016-1247-zjournal_volume
198pub_type
杂志文章abstract::A novel thermotolerant strain of the achlorophyllous micro-alga Prototheca was isolated from a hot spring. The isolate was found to produce an appreciable amount of ethanol and CO2 from glucose under anoxic conditions at both 25 and 40 degrees C; this type of alcohol fermentation has not yet been reported in the genus...
journal_title:Archives of microbiology
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journal_title:Archives of microbiology
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journal_title:Archives of microbiology
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更新日期:1975-05-05 00:00:00
abstract::An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 degrees C and 5.4 g NaCl l(-1). The strain grows heterotrophically on complex pr...
journal_title:Archives of microbiology
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更新日期:1999-08-01 00:00:00
abstract::Mutants resistance to various combinations of threonine, lysine and/or their analogs were obtained and characterized in Pseudomonas acidovorans. In particular, mutants resistant to aminoethylcysteine had a dihydrodipicolinate synthetase insensitive to lysine inhibition whereas mutants resistant to threonine plus a low...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446845
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pub_type: 杂志文章,评审
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更新日期:1997-09-01 00:00:00
abstract::Cadaverin was more readily incorporated than lysine into arthrobactin from Arthrobacter pascens and into ferrioxamin E from Streptomyces glaucescens. From a racemic mixture only the L-isomer of lysine is incorporated. The L-lysine decarboxylase activity was measured in vivo and in vitro. The enzyme from Arthrobacter p...
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pub_type: 杂志文章
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