Abstract:
:The main property of an Amt- (ammonium transport negative) mutant of Klebsiella pneumoniae is its inability to accumulate NH4+ intracellularly. When growing on nitrogen sources other than NH4+, the mutant constantly looses NH3 by diffusion. This loss results in poor growth. The NH3 excretion suggests the existence of a futile cycle (NH3 loss/NH4+ reabsorption) in the wild type and possibly other bacterial strains, which do not constantly excrete NH3.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Castorph H,Kleiner Ddoi
10.1007/BF00402008subject
Has Abstractpub_date
1984-10-01 00:00:00pages
245-7issue
2-3eissn
0302-8933issn
1432-072Xjournal_volume
139pub_type
杂志文章abstract::Twelve Cry1 and two Cry9 delta-endotoxins from Bacillus thuringiensis were tested for their activity against black cutworm ( Agrotis ipsilon). A. ipsilon was not susceptible to many toxins, but three toxins had significant activity. Cry9Ca was the most toxic, followed by Cry1Aa and Cry1Fb. Hybrids between these three ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0543-6
更新日期:2003-05-01 00:00:00
abstract::We previously constructed an arabinose-inducible recombinant Bifidobacterium longum that could efficiently express secreted IFN-alpha2b in vitro (Deng et al. in Arch Microbiol 191:681-686, 2009). Here, we investigated the influence of oral pBAD-SPIFN-transformed B. longum on immunomodulation and IFN-induced Mx1 gene t...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-010-0589-1
更新日期:2010-08-01 00:00:00
abstract::In beta-lactam-antibiotic-producing fungi, such as Aspergillus (Emericella) nidulans, L-alpha-aminoadipic acid is the branching point of the lysine and penicillin biosynthesis pathways. To obtain a deeper insight into the regulation of lysine biosynthesis genes, the regulation of the A. nidulans lysF gene, which encod...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030000246
更新日期:2001-02-01 00:00:00
abstract::A triazine dye, Cibacron blue 3G-A (CB), is an inhibitor of cell separation of staphylococcal spp. therefore, we examined the effect of CB on growth of gram-positive bacteria other than Staphylococcus. CB added to the medium of growing cultures of strains of genus Micrococcus, Streptococcus, Lactobacillus and Bacillus...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00248617
更新日期:1991-01-01 00:00:00
abstract::Fifty isolates belonging to the six validly described species of the genus Thermus (T. aquaticus, T. filiformis, T. thermophilus, T. scotoductus, T. brockianus, and T. oshimai) isolated from hot springs of different geographical areas were compared using macrorestriction analysis of genomic DNA and ribotyping. With th...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050474
更新日期:1997-08-01 00:00:00
abstract::The prpC and prkC genes encode two proteins with opposing activities, eukaryotic-like protein phosphatase and protein kinase involved in the sporulation and biofilm formation processes. The prpC gene overlaps 3 bp of the prkC gene; prkC is followed by the essential gene, yloQ. The organisation of the prpC, prkC and yl...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0015-2
更新日期:2005-09-01 00:00:00
abstract::AMP-degrading pathways in Azotobacter vinelandii cells were investigated. AMP nucleosidase (EC 3.2.2.4) was rapidly synthesized and reached a maximum at 24 h, while the activity of 5'-nucleotidase (EC 3.1.3.5) specific for AMP, which was negligible during the logarithmic phase of the growth, first appeared in 24 h-cul...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00406162
更新日期:1980-12-01 00:00:00
abstract::Small heat-shock proteins are molecular chaperones that bind and prevent aggregation of nonnative proteins. They also associate with membranes. In this study, we show that the small heat-shock protein HspA plays a protective role under oxidative stress in the cyanobacterium Synechococcus elongatus strain ECT16-1, whic...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-009-0457-z
更新日期:2009-04-01 00:00:00
abstract::Two styrene monooxygenase types, StyA/StyB and StyA1/StyA2B, have been described each consisting of an epoxidase and a reductase. A gene fusion which led to the chimeric reductase StyA2B and the occurrence in different phyla are major differences. Identification of SMOA/SMOB-ADP1 of Acinetobacter baylyi ADP1 may enlig...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-1022-y
更新日期:2014-12-01 00:00:00
abstract::Fermentative degradation of phenol was studied using a non-methanogenic, pasteurised enrichment culture containing two morphologically different bacteria. Phenol was fermented to benzoate, acetate and butyrate and their relative occurrence depended on the concentration of hydrogen. Proportionately more benzoate was fo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030000160
更新日期:2000-05-01 00:00:00
abstract::Ostracods collected from shallow coral reefs in the Bahamas were found to exhibit blue light-stimulated orange fluorescence at night. Fluorescent spectra revealed the presence of orange fluorescence with a maximum emission at ~595 nm on the carapace of these ostracods, while scanning electron microscopy revealed a mor...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-013-0911-9
更新日期:2013-09-01 00:00:00
abstract::Curcumin is a yellow-orange compound derived from the root of Curcuma longa (Zingiberaceae family), that has been used as a medicine, spice and coloring agent. Curcumin has proved nontoxic in a number of cell culture and whole animal studies. Curcumin has, however, been reported to have bactericidal effects at very hi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00414437
更新日期:1989-01-01 00:00:00
abstract::TonB-dependent receptors in concert with the TonB-ExbB-ExbD protein complex are responsible for the uptake of iron and substances such as vitamin B12 in several bacterial species. In this study, Tn5 mutagenesis of the sugarcane endophytic bacterium Gluconacetobacter diazotrophicus led to the isolation of a mutant with...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-1045-4
更新日期:2015-03-01 00:00:00
abstract::Mycoplasma pneumoniae and Mycoplasma genitalium are important causative agents of infections in humans. Like all other mycoplasmas, these species possess genomes that are significantly smaller than that of other prokaryotes. Moreover, both organisms possess an exceptionally compact set of DNA recombination and repair-...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-020-02041-4
更新日期:2020-09-24 00:00:00
abstract::Light-limited batch cultures of Synechococcus spp. strains PCC 6301 and WH 8103 exhibited a positive correlation between the specific growth rate and the cellular content of both RNA and DNA. The ratio of RNA to DNA increased with the growth rate in Synechococcus sp. strain WH 8103, as it does in heterotrophic bacteri...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050634
更新日期:1998-09-01 00:00:00
abstract::A thermostable homodimeric isocitrate dehydrogenase from the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus was purified and characterized. The mol. mass of the isocitrate dehydrogenase subunit was 42 kDa as determined by SDS-PAGE. Following separation by SDS-PAGE, A. fulgidus isocitrate dehydrogen...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050516
更新日期:1997-11-01 00:00:00
abstract::The thermotolerant methylotroph Bacillus sp. C1 possesses a novel NAD-dependent methanol dehydrogenase (MDH), with distinct structural and mechanistic properties. During growth on methanol and ethanol, MDH was responsible for the oxidation of both these substrates. MDH activity in cells grown on methanol or glucose wa...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00245161
更新日期:1992-01-01 00:00:00
abstract::The haloalkaliphilic archaeon Natronobacterium vacuolatum forms cylinder-shaped gas vesicles throughout the growth cycle when grown in media containing 15-25% NaCl. Cells cultivated in media containing 13% NaCl are, however, gas-vesicle-free. The major gas vesicle structural protein, nv-GvpA, was detected by an antise...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050465
更新日期:1997-07-01 00:00:00
abstract::The TTA codon, one of the six available codons for the amino acid leucine, is the rarest codon among the high GC genomes of Actinobacteria including Frankia. This codon has been implicated in various regulatory mechanisms involving secondary metabolism and morphological development. TTA-mediated gene regulation is wel...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-011-0731-8
更新日期:2012-01-01 00:00:00
abstract::Clostridium butyricum is widely used to produce organic solvents such as ethanol, butanol and acetone. We sequenced the entire genome of C. butyricum INCQS635 by using Ion Torrent technology. We found a high contribution of sequences assigned for carbohydrate subsystems (15-20 % of known sequences). Annotation based o...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-015-1166-4
更新日期:2016-03-01 00:00:00
abstract::Purified flagellar filaments isolated from six methanogens were composed of multiple flagellins. Two flagellins were present in Methanococcus deltae (Mr = 34,000 and 32,000), Methanoculleus marisnigri (Mr = 31,000 and 25,500) and Methanococcus jannaschii (Mr = 31,000 and 27,500), three in Methanothermus fervidus (Mr =...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00276766
更新日期:1992-01-01 00:00:00
abstract::Recently, we reported the synthesis of five bacteriocin-like inhibitor substances (Bt-BLIS: morricin 269, kurstacin 287, kenyacin 404, entomocin 420, and tolworthcin 524) by Mexican strains of Bacillus thuringiensis. Here we show that, collectively, these Bt-BLIS have a moderate to broad spectrum of antibacterial acti...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-008-0414-2
更新日期:2008-12-01 00:00:00
abstract::The interaction between Shigella dysenteriae or Shigella sonnei and Acanthamoeba castellanii was studied by viable counts, gentamicin assay and electron microscopy. The result showed that Shigella dysenteriae or Shigella sonnei grew and survived in the presence of amoebae for more than 3 weeks. Gentamicin assay showed...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-008-0422-2
更新日期:2009-01-01 00:00:00
abstract::The determination of enzymatic activities in cell-free extracts of Acidaminococcus fermentans and Peptostreptococcus asaccharolyticus led to a refined scheme for the pathway of glutamate fermentation via (R)-2-hydroxyglutarate to acetate and butyrate. From the ratio of these products the amount of ATP generated by sub...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00447055
更新日期:1985-07-01 00:00:00
abstract::As the protein databases continue to expand at an exponential rate, fed by daily uploads from multiple large scale genomic and metagenomic projects, the problem of assigning a function to each new protein has become the focus of significant research interest in recent times. Herein, we review the most recent advances ...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-010-0549-9
更新日期:2010-03-01 00:00:00
abstract::Acinetobacter strain IVS-B aerobically grows on isovalerate as sole carbon and energy source. Isovalerate is metabolised via isovaleryl-CoA, an intermediate of the oxidative (S)-leucine degradation pathway. A 3-methylglutaconyl-CoA hydratase (EC 4.2.1.18) was purified 65-fold to apparent homogeneity from cell-free ext...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-006-0095-7
更新日期:2006-05-01 00:00:00
abstract::The fate of 35S label was studied during cystine degradation by mycelia of the dermatophytic fungus Microsporum gypseum. Excess free cystine in the medium was readily taken up and its sulfur moiety excreted as inorganic sulfate and sulfite. At intervals after 3-60 min of incubation with 35S cystine the products of cys...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446777
更新日期:1986-07-01 00:00:00
abstract::To elucidate the physiological adaptation of Escherichia coli due to cra gene knockout, a total of 3,911 gene expressions were investigated by DNA microarray for continuous culture. About 50 genes were differentially regulated for the cra mutant. TCA cycle and glyoxylate shunt were down-regulated, while pentose phosph...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-008-0406-2
更新日期:2008-11-01 00:00:00
abstract::Effects of different flavonoids on various bacterial strains have been extensively reported; however, the mechanism(s) of their action on bacterial cells remain largely elusive. In this study, the antibacterial mechanism of soybean isoflavone (SI) on Staphylococcus aureus is systematically investigated using 4'6-diami...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-010-0617-1
更新日期:2010-11-01 00:00:00
abstract::The family Chlamydiaceae contains nine species pathogenic to humans and animals, but their routine identification is hampered by inadequate detection methods. In an attempt to find a new region for PCR detection and discrimination of the Chlamydiaceae species, the 3' end of the omp2 gene of Chlamydiaceae has been exam...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0757-2
更新日期:2005-03-01 00:00:00