Abstract：
:In beta-lactam-antibiotic-producing fungi, such as Aspergillus (Emericella) nidulans, L-alpha-aminoadipic acid is the branching point of the lysine and penicillin biosynthesis pathways. To obtain a deeper insight into the regulation of lysine biosynthesis genes, the regulation of the A. nidulans lysF gene, which encodes homoaconitase, was studied. Band-shift assays indicated that the A. nidulans multimeric CCAAT-binding complex AnCF binds to two of four CCAAT motifs present in the lysF promoter region. AnCF consists at least of three different subunits, designated HapB, HapC, and HapE. In both a delta hapB and a delta hapC strain, the expression of a translational lysF-lacZ gene fusion integrated in single copy at the chromosomal argB gene locus was two to three-fold higher than in a wild-type strain. These data show that AnCF negatively regulates lysF expression. The results of Northern blot analysis and lysF-lacZ expression analysis did not indicate a lysine-dependent repression of lysF expression. Furthermore, mutational analysis of the lysF promoter region revealed that two GATA sites matching the GATA consensus sequence HGATAR positively affected lysF-lacZ expression. Results of Northern blot analysis also excluded that the global nitrogen regulator AreA is the responsible trans-acting GATA-binding factor.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Weidner G,Steidl S,Brakhage AAdoi
10.1007/s002030000246keywords:
subject
Has Abstractpub_date
2001-02-01 00:00:00pages
122-32issue
2eissn
0302-8933issn
1432-072Xjournal_volume
175pub_type
杂志文章abstract：:Geobacter species such as G. bremensis, G. pelophilus, and G. sulfurreducens are obligately anaerobic and grow in anoxic, non-reduced medium by fast reduction of soluble ferric citrate. In contrast, insoluble ferrihydrite was either only slowly or not reduced when supplied as electron acceptor in similar growth experi...
journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：2004-10-01 00:00:00
abstract：:Saccharomyces cerevisiaepresents two alternative vegetative forms of growth, switching between yeast forms to pseudohyphal forms depending on the specific environmental conditions. To identify genes involved in cell wall morphogenesis, a haploid S. cerevisiae monomorphic mutant, W27, which exhibits pseudohyphal growth...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0400-z
更新日期：2002-04-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00416972
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00690235
更新日期：1976-11-02 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：2002-06-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
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更新日期：2010-03-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050526
更新日期：1997-12-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00307769
更新日期：1994-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446549
更新日期：1976-12-01 00:00:00
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更新日期：2000-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00511216
更新日期：1980-06-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446663
更新日期：1977-02-04 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050678
更新日期：1998-12-01 00:00:00
abstract：:The association of nucleoside triphosphate molecules and calcium ions with purified particles of mycobacteriophage 13 has been documented. The content of nucleoside triphosphate has been determined to be 118 molecules per phage particle by equilibrium dialysis against labelled ATP or 148 molecules per phage particle b...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00527071
更新日期：1981-09-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050535
更新日期：1998-01-01 00:00:00
abstract：:The natural role of antibiotics in the ecology of Streptomyces is debated and still largely unknown. The predatory myxobacteria and many other genera of prokaryotic epibiotic and wolfpack predators across different taxa possess secondary metabolites with antimicrobial action, and these compounds have a role in predati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-0961-7
更新日期：2014-04-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00427847
更新日期：1977-10-24 00:00:00
abstract：:This study compared rhizobacterial communities in pepper greenhouses under a paddy-upland (rice-pepper) rotational system (PURS) and a monoculture repeated cropping system (RCS) and examined adverse effects of high salinity on soil properties. The following soil properties were analyzed: electrical conductivity (EC), ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1304-7
更新日期：2017-03-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446920
更新日期：1989-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
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更新日期：2010-10-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00415614
更新日期：1983-10-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：2015-03-01 00:00:00
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journal_title：Archives of microbiology
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doi：10.1007/s00203-010-0562-z
更新日期：2010-05-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050340
更新日期：1996-06-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF01682989
更新日期：1996-12-01 00:00:00
abstract：:During the course of isolating rare actinobacteria from unexplored habitats, strain CH32T was obtained from an arid soil sample in eastern Anatolia, Turkey. Polyphasic characterization and comprehensive genome analyses showed that the strain is a member of the genus Nonomuraea and it is closely related to Nonomuraea g...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01941-9
更新日期：2020-10-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-015-1117-0
更新日期：2015-08-01 00:00:00
abstract：:The filamentous fungus Penicillium italicum when grown in a synthetic medium, produced and secreted 1,6-beta-glucanase into the culture medium. This enzyme has been partially purified by gel filtration. After this step the active fractions were free of 1,3-beta-glucanase, alpha-amylase and beta-glucosidase activities....
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425066
更新日期：1979-06-01 00:00:00
abstract：:Rhizopus oryzae has long been used for enzyme production (e.g., glucoamylase and lipase), organic acid synthesis, and various fermented food applications. In this work, we describe a set of plasmid-based expression vectors that can be used for the production of heterologous proteins in R. oryzae. Three plasmid vectors...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0121-9
更新日期：2006-07-01 00:00:00
abstract：:Glucose-limited continuous cultures were used to analyze sigmaB activity at decreasing growth rates. Expression of the sigmaB-dependent genes gsiB and ctc started to increase at a growth rate of 0.2 h-1, and both genes were induced approximately fivefold at a growth rate of 0.1 h-1 as compared to expression at the max...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050731
更新日期：1999-05-01 00:00:00
