Cloning and characterization of two novel DNases from Streptococcus pyogenes.


:The proteins in the culture supernatant (exoproteins) from Streptococcus pyogenes serotype M1 were separated by two-dimensional gel electrophoresis, and their N-terminal amino acid sequences were determined. The amino acid sequences were compared to sequences in the S. pyogenes genome database. The coding sequence showed similarity to sequences of two genes, mf2-v ( mf2 variant) and mf3, which had sequence similarity to genes encoding mitogenic factor (MF); MF has DNase activity. The recombinant genes were expressed in Escherichia coli and the proteins were synthesized. Mf2-v and Mf3 had DNase activity. The activity of Mf2-v was localized to the C-terminal half of the protein. The mf3 gene was shown to be present in most clinically isolated strains of S. pyogenes tested, and the mf2gene was detected in 20% of the isolates. The products of the mf2 and mf3 genes in clinically isolated S. pyogenes strains were thus shown to be DNases.


Arch Microbiol


Archives of microbiology


Hasegawa T,Torii K,Hashikawa S,Iinuma Y,Ohta M





Has Abstract


2002-06-01 00:00:00












  • Oxidation and ring cleavage of dibenzofuran by the filamentous fungus Paecilomyces lilacinus.

    abstract::The ability of the imperfect soil fungus Paecilomyces lilacinus to transform the environmental pollutant dibenzofuran was investigated. Transformation of dibenzofuran and related derivatives lead to 14 products, which were identified by UV spectroscopy, mass spectrometry, and proton nuclear magnetic resonance spectros...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Gesell M,Hammer E,Mikolasch A,Schauer F

    更新日期:2004-09-01 00:00:00

  • Identification and molecular characterization of twin-arginine translocation system (Tat) in Xanthomonas oryzae pv. oryzae strain PXO99.

    abstract::Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, one of the most widespread and destructive bacterial diseases in rice. This study identified and characterized the contribution of the twin-arginine translocation (Tat) pathway to motility, chemotaxis, extracellular polysaccharide (EPS) production and virulen...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Chen L,Hu B,Qian G,Wang C,Yang W,Han Z,Liu F

    更新日期:2009-02-01 00:00:00

  • Morphological, physiological, and molecular characterization of actinomycetes isolated from dry soil, rocks, and monument surfaces.

    abstract::In an extended study on the biodiversity of rock-dwelling bacteria, the colony and cell morphology, physiology, protein patterns, and 16S rDNA sequences of 17 bacterial strains isolated from different surfaces of rocks, stones, and monuments and from various geographical locations were characterized. All except one st...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Eppard M,Krumbein WE,Koch C,Rhiel E,Staley JT,Stackebrandt E

    更新日期:1996-07-01 00:00:00

  • Evaluation of mtr cluster expression in Shewanella RCRI7 during uranium removal.

    abstract::In recent years, bioremediation is considered as an efficient method to remove the pollutants from the industrial wastewater. In this study, quantitative gene expressions (Real-time RT-PCR) of mtr gene cluster (mtrA, mtrB, mtrC, mtrD, mtrE, mtrF and omcA) in five different uranium concentrations (0.1, 0.25, 0.5, 1 and...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Ghasemi R,Fatemi F,Mir-Derikvand M,Zarei M

    更新日期:2020-12-01 00:00:00

  • Purification, characterization and gene cloning of isoeugenol-degrading enzyme from Pseudomonas putida IE27.

    abstract::An isoeugenol-degrading enzyme was purified to homogeneity from Pseudomonas putida IE27, an isoeugenol-assimilating bacterium. The purified enzyme was a 55 kDa monomer and catalyzed the initial step of isoeugenol degradation, the oxidative cleavage of the side chain double-bond of isoeugenol, to form vanillin. Another...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Yamada M,Okada Y,Yoshida T,Nagasawa T

    更新日期:2007-06-01 00:00:00

  • Enzymic arrangement and allosteric regulation of the aromatic amino acid pathway in Neisseria gonorrhoeae.

    abstract::The pathway construction and allosteric regulation of phenylalanine and tyrosine biosynthesis was examined in Neisseria gonorrhoeae. A single 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase enzyme sensitive to feedback inhibition by L-phenylalanine was found. Chorismate mutase and prephenate dehydratase ap...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Berry A,Jensen RA,Hendry AT

    更新日期:1987-01-01 00:00:00

  • Repression of toxin production by tryptophan in Clostridium botulinum type E.

    abstract::Of the seven amino acids required by Clostridium botulinum type E, tryptophan is the most essential and may provide the cell with nitrogen. The addition of excess tryptophan (10-20 mM) or other nitrogenous nutrients to minimal growth medium markedly decreased toxin formation but did not affect growth in C. botulinum t...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Leyer GJ,Johnson EA

    更新日期:1990-01-01 00:00:00

  • Fish borne Edwardsiella tarda eha involved in the bacterial biofilm formation, hemolytic activity, adhesion capability and pathogenicity.

    abstract::Edwardsiella tarda (E. tarda) is distributed widely in a variety of hosts including humans, other mammals and fish, and it is worthwhile to notice that E. tarda -caused fish infections lead to the most important bacterial disease in fish. Considering Eha acting as a transcriptional regulator in E. tarda strain ET13 ha...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Hassan HA,Ding X,Zhang X,Zhu G

    更新日期:2020-05-01 00:00:00

  • Microbial biosynthesis of halometabolites.

    abstract::Halometabolites are compounds that are commonly found in nature and they are produced by many different organisms. Whereas bromometabolites can mainly be found in the marine environment, chlorometabolites are predominately produced by terrestrial organisms; iodo- and fluorocompounds are only produced infrequently. The...

    journal_title:Archives of microbiology

    pub_type: 杂志文章,评审


    authors: van Pée KH

    更新日期:2001-04-01 00:00:00

  • The glycolytic genes pfk and pyk from Lactobacillus casei are induced by sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system and repressed by CcpA.

    abstract::In Lactobacillus casei BL23, phosphofructokinase activity was higher in cells utilizing sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system (PTS). The phosphofructokinase gene (pfk) was cloned from L. casei and shown to be clustered with the gene encoding pyruvate kinase (pyk). pfk and pyk ge...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Viana R,Pérez-Martínez G,Deutscher J,Monedero V

    更新日期:2005-09-01 00:00:00

  • Paenibacillus turpanensis sp. nov., isolated from a salt lake of Turpan city in Xinjiang province, north-west China.

    abstract::Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach. The optimum growth temperature was 37 °C and the optimum pH was 7.5-...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Yang L,Huang HW,Wang Y,Kou YR,Yin M,Li Y,Wang XQ,Zhao GF,Zhu WY,Tang SK

    更新日期:2021-01-01 00:00:00

  • Purification and characterization of benzyl alcohol- and benzaldehyde- dehydrogenase from Pseudomonas putida CSV86.

    abstract::Pseudomonas putida CSV86 utilizes benzyl alcohol via catechol and methylnaphthalenes through detoxification pathway via hydroxymethylnaphthalenes and naphthaldehydes. Based on metabolic studies, benzyl alcohol dehydrogenase (BADH) and benzaldehyde dehydrogenase (BZDH) were hypothesized to be involved in the detoxifica...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Shrivastava R,Basu A,Phale PS

    更新日期:2011-08-01 00:00:00

  • Virulent strains of Salmonella enteritidis disrupt the epithelial barrier of Caco-2 and HEp-2 cells.

    abstract::To confirm the existence in nature of Salmonella enteritidis strains of different degrees of virulence and to elucidate the mechanisms underlying the effects of such strains on the epithelial barrier function, the consequences of infection of Caco-2 cells and HEp-2 cells with 15 S. enteritidis strains in a chicken inf...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Solano C,Sesma B,Alvarez M,Urdaneta E,Garcia-Ros D,Calvo A,Gamazo C

    更新日期:2001-01-01 00:00:00

  • The Bradyrhizobium japonicum fixGHIS genes are required for the formation of the high-affinity cbb3-type cytochrome oxidase.

    abstract::We report structural and functional analyses of the Bradyrhizobium japonicum fixGHIS genes, which map immediately downstream of the fixNOQP operon for the symbiotically essential cbb3-type heme-copper oxidase complex. Expression of fixGHIS, like that of fixNOQP, is strongly induced in cells grown microaerobically or a...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Preisig O,Zufferey R,Hennecke H

    更新日期:1996-05-01 00:00:00

  • The interaction of copper ions with Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli: an X-ray absorption near-edge structure (XANES) spectroscopy study.

    abstract::The antimicrobial properties of copper ions have been known for a long time. However, the exact mechanism of action of the transition metal on microorganisms has long been unclear. X-ray absorption near-edge structure (XANES) spectroscopy at the Cu K edge allows the determination of copper speciation in Staphylococcus...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Zanzen U,Bovenkamp-Langlois L,Klysubun W,Hormes J,Prange A

    更新日期:2018-04-01 00:00:00

  • Direct studies of dikaryotization in Schizophyllum commune. II. Behavior and fate of multikaryotic hyphase.

    abstract::The growth, duplication and fate of multikaryotic hyphae bearing true clamp connections, as derived from compatible matings of Schizophyllum commune, were studied by phase contrast microscopy. The nuclei (N) of multikaryotic apices maintained a near central position during hyphal growth. True clamp connection formatio...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Niederpruem DJ

    更新日期:1980-12-01 00:00:00

  • Synergistic iron reduction and citrate dissimilation by Shewanella alga and Aeromonas veronii

    abstract::Two bacterial isolates from Great Bay Estuary, New Hampshire, in co-culture carried out anaerobic dissimilation of citric acid with Fe(III) as the terminal electron acceptor. Neither isolate oxidized citrate with Fe(III) anaerobically in axenic culture. The Fe(III) reducer, Shewanella alga strain BrY, did not grow ana...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Knight V V,Caccavo F,Wudyka S,Blakemore R

    更新日期:1996-10-17 00:00:00

  • The role of the cellulolytic high molecular mass (HMM) complex of the anaerobic fungus Piromyces sp. strain E2 in the hydrolysis of microcrystalline cellulose.

    abstract::The anaerobic fungus Piromyces sp. strain E2 produces extracellular cellulolytic enzymes present both in a high molecular mass (HMM) complex or as individual proteins. Although the HMM complex was present in the culture fluid during all growth stages, the highest amounts of complex were obtained when cultures were har...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Dijkerman R,Op den Camp HJ,Van der Drift C,Vogels GD

    更新日期:1997-02-01 00:00:00

  • CO2-dependent fermentation of phenol to acetate, butyrate and benzoate by an anaerobic, pasteurised culture.

    abstract::Fermentative degradation of phenol was studied using a non-methanogenic, pasteurised enrichment culture containing two morphologically different bacteria. Phenol was fermented to benzoate, acetate and butyrate and their relative occurrence depended on the concentration of hydrogen. Proportionately more benzoate was fo...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Karlsson A,Ejlertsson J,Svensson BH

    更新日期:2000-05-01 00:00:00

  • Characterization and synthesis regulation of Penicillium italicum 1,6-beta-glucanase.

    abstract::The filamentous fungus Penicillium italicum when grown in a synthetic medium, produced and secreted 1,6-beta-glucanase into the culture medium. This enzyme has been partially purified by gel filtration. After this step the active fractions were free of 1,3-beta-glucanase, alpha-amylase and beta-glucosidase activities....

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Santos T,Nombela C,Villanueva JR,Larriba G

    更新日期:1979-06-01 00:00:00

  • Flow cytometric screening and isolation of Escherichia coli clones which express surface antigens of the oil-degrading microorganism Acinetobacter calcoaceticus RAG-1.

    abstract::Flow cytometry (FCM) in conjunction with immunocytochemical-labeling was used to analyze and screen a population of Escherichia coli clones containing a genomic library from the oil-degrading microorganism Acinetobacter calcoaceticus RAG-1 surface antigens. Reconstruction experiments using mixed populations indicated ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Minas W,Sahar E,Gutnick D

    更新日期:1988-01-01 00:00:00

  • Characterization of a eukaryotic-like protein kinase, DspB, with an atypical catalytic loop motif from Myxococcus xanthus.

    abstract::Serine (Ser)/threonine (Thr) or tyrosine (Tyr) protein kinases in eukaryotes contain RDxKxxN or RDx(A/R)A(A/R)N sequences, respectively, in the catalytic loop. Myxococcus xanthus DspB is a dual-specificity kinase that contains an atypical sequence, RDVAQKN, in the catalytic loop. The DspB mutant (A165K), which contain...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Kimura Y,Urata M

    更新日期:2016-04-01 00:00:00

  • Periplasmic superoxide dismutases in Aquaspirillum magnetotacticum.

    abstract::Aquaspirillum magnetotacticum MS-1 cells cultured microaerobically (dissolved O2 tension 1% of saturation), expressed proteins with superoxide dismutase (SOD) activity. The majority (roughly 95%) of total cell superoxide dismutase activity was located in the cell periplasm with little or no activity in the cell cytopl...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Short KA,Blakemore RP

    更新日期:1989-01-01 00:00:00

  • Penicillin-induced changes in the cell wall composition of Staphylococcus aureus before the onset of bacteriolysis.

    abstract::To analyze if chemical cell wall alterations contribute to penicillin-induced bacteriolysis, changes in the amount, stability, and chemical composition of staphylococcal cell walls were investigated. All analyses were performed before onset of bacteriolysis i.e. during the first 60 min following addition of different ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Sidow T,Johannsen L,Labischinski H

    更新日期:1990-01-01 00:00:00

  • Further studies on RpoS in enterobacteria: identification of rpoS in Enterobacter cloacae and Kluyvera cryocrescens.

    abstract::RpoS, the alternative sigma factor sigma(s), is important for bacterial survival under extreme conditions. Many enterobacteria are opportunistic human pathogens and their ability to survive in a changing environment could be an essential step for their virulence. To determine the presence of this gene in enteric bacte...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Martínez-García E,Tormo A,Navarro-Llorens JM

    更新日期:2001-06-01 00:00:00

  • Autoinduction of bacterial luciferase. Occurrence, mechanism and significance.

    abstract::The synthesis of the luminous system of the marine luminous bacterium Photobacterium fischeri is subject to a complex, self-regulated control system called autoinduction. The bacteria produce an autoinducer which accumulates in the medium at a constant rate (as a function of cell growth). When autoinducer reaches a cr...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Nealson KH

    更新日期:1977-02-04 00:00:00

  • Utilization of D-amino acids by dadR mutants of Salmonella typhimurium.

    abstract::Utilization of D-amino acids being substrates of D-amino acid dehydrogenase of Salmonella typhimurium was examined. The experiments were done with wild type strains and the mutants dadA missing the enzyme activity and dadR in which its synthesis is released from catabolite repression. Growth on D-tryptophan, D-histidi...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Wild J,Filutowicz M,Kłopotowski T

    更新日期:1978-07-01 00:00:00

  • 3-Methylglutaconyl-CoA hydratase from Acinetobacter sp.

    abstract::Acinetobacter strain IVS-B aerobically grows on isovalerate as sole carbon and energy source. Isovalerate is metabolised via isovaleryl-CoA, an intermediate of the oxidative (S)-leucine degradation pathway. A 3-methylglutaconyl-CoA hydratase (EC was purified 65-fold to apparent homogeneity from cell-free ext...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Mack M,Liesert M,Zschocke J,Peters V,Linder D,Buckel W

    更新日期:2006-05-01 00:00:00

  • Aromatic amino acid biosynthesis in Alcaligenes eutrophus H16. II. The isolation and characterization of mutants auxotrophic for phenylalanine and tyrosine.

    abstract::1. Mutants derived from the hydrogen bacterium Alcaligenes eutrophus strain H 16 auxotrophic for phenylalanine and tyrosine were isolated employing mutagenic agents (EMS, nitrite), the colistine counterselection technique and the "pin-point" isolation method. Three different types of mutants were found: (1) Mutants, r...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Friedrich B,Schlegel HG

    更新日期:1975-04-07 00:00:00

  • Photokilling of bacteria by the natural dye curcumin.

    abstract::Curcumin is a yellow-orange compound derived from the root of Curcuma longa (Zingiberaceae family), that has been used as a medicine, spice and coloring agent. Curcumin has proved nontoxic in a number of cell culture and whole animal studies. Curcumin has, however, been reported to have bactericidal effects at very hi...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Dahl TA,McGowan WM,Shand MA,Srinivasan VS

    更新日期:1989-01-01 00:00:00