Abstract:
:Pyruvate kinase (ATP: pyruvate phosphotransferase (EC 2.7.1.40) was partially purified from both autotrophically and heterotrophically grown Paracoccus denitrificans. The organism grown under heterotrophic conditions contains four times more pyruvate kinase than under autotrophic conditions. The enzyme isolated from both sources exhibited sigmoidal kinetics for both phosphoenolpyruvate (PEP) and ADP. The apparent Km for ADP and PEP in the "autotrophic" enzyme were 0.63 mM ADP and 0.25 mM PEP. The effect of several low molecular weight metabolites on the pyruvate kinase activity was investigated. Ribose-5-phosphate, glucose-6-phosphate and AMP stimulated the reaction at low ADP levels; this stimulation was brought about by an alteration in the apparent Km for ADP. The pyruvate kinases differ in their response to adenine nucleotides, but both preparations seem to be under adenylate control. The results are discussed in relation to the role of pyruvate kinase as a regulatory enzyme in P. denitrificans grown under both autotrophic and heterotrophic conditions.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Slabas AR,Whatley FRdoi
10.1007/BF00427847subject
Has Abstractpub_date
1977-10-24 00:00:00pages
67-71issue
1eissn
0302-8933issn
1432-072Xjournal_volume
115pub_type
杂志文章abstract::AMP-degrading pathways in Azotobacter vinelandii cells were investigated. AMP nucleosidase (EC 3.2.2.4) was rapidly synthesized and reached a maximum at 24 h, while the activity of 5'-nucleotidase (EC 3.1.3.5) specific for AMP, which was negligible during the logarithmic phase of the growth, first appeared in 24 h-cul...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00406162
更新日期:1980-12-01 00:00:00
abstract::A new, phylogenetically distinct, dissimilatory, Fe(III)-reducing bacterium was isolated from surface sediment of a hydrocarbon-contaminated ditch. The isolate, designated strain PAL-1, was an obligately anaerobic, non-fermentative, motile, gram-negative vibrio. PAL-1 grew in a defined medium with acetate as electron ...
journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:1996-06-01 00:00:00
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journal_title:Archives of microbiology
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更新日期:1986-03-01 00:00:00
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journal_title:Archives of microbiology
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journal_title:Archives of microbiology
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更新日期:2006-06-01 00:00:00
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journal_title:Archives of microbiology
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更新日期:2014-02-01 00:00:00
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journal_title:Archives of microbiology
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journal_title:Archives of microbiology
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更新日期:2003-12-01 00:00:00
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journal_title:Archives of microbiology
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更新日期:2004-10-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00447300
更新日期:1975-06-20 00:00:00
abstract::The fast-growing Rhizobium sp. strain NGR234, isolated from Papua New Guinea, and 13 strains of Sinorhizobium fredii, isolated from China and Vietnam, were fingerprinted by means of RAPD, REP, ERIC and ARDRA. ERIC, REP and RAPD markers revealed a considerable genetic diversity among fast-growing rhizobia. Chinese isol...
journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:2009-04-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2020-05-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00248617
更新日期:1991-01-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0049-5
更新日期:2006-01-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:2007-08-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00276476
更新日期:1994-01-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
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journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:1998-10-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:2001-11-01 00:00:00
abstract::Flow cytometry (FCM) in conjunction with immunocytochemical-labeling was used to analyze and screen a population of Escherichia coli clones containing a genomic library from the oil-degrading microorganism Acinetobacter calcoaceticus RAG-1 surface antigens. Reconstruction experiments using mixed populations indicated ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00422282
更新日期:1988-01-01 00:00:00
abstract::Although, there have been many published bacterial strains aerobically degrading the heterocyclic amine compounds, only one strain to date has been reported to degrade pyrrolidine under denitrifying conditions. In this study, denitrifying bacteria degrading pyrrolidine and piperidine were isolated from diverse geologi...
journal_title:Archives of microbiology
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更新日期:2009-04-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:1989-01-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:1983-02-01 00:00:00
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journal_title:Archives of microbiology
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更新日期:2000-10-01 00:00:00
abstract::Carbon-14 was incorporated into oxalate and CO2 from either citrate-1,5-14C, succinate-1,4-14C, or fumarate-1,4-14C by cultures of Aspergillus niger pregrown on a medium which contained glucose as the sole carbon source and which did not allow citrate accumulation. In cell-free extracts of mycelium forming oxalate and...
journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:1975-06-22 00:00:00
abstract::The clones generated in a sequencing project represent a resource for subsequent analysis of the organism whose genome has been sequenced. We describe an interrelated group of cloning vectors that either integrate into the genome or replicate, and that enhance the utility, for developmental and other studies, of the c...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0276-z
更新日期:2007-12-01 00:00:00
abstract::Enzymes that are regulated by the ferredoxin/thioredoxin system in chloroplasts--fructose-1,6-bis-phosphatase (FBPase), sedoheptulose-1,7-bisphosphatase (SBPase), and phosphoribulokinase (PRK)--were partially purified from two different types of photosynthetic prokaryotes (cyanobacteria, purple sulfur bacteria) and te...
journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:1984-10-01 00:00:00