Abstract：
:A LD-carboxypeptidase from Escherichia coli K 12 was isolated by Tris-EDTA treatment and purified to electrophoretic homogeneity by DEAE-cellulose chromatography. The enzyme has a molecular weight of approximately 12,000 as determined by sodium dodecyl sulfate-polyacrylamide electrophoresis and by Sephadex G-100 gel filtration. The studies of the substrate specificity of the enzyme revealed that UDP-MurNAc-tetrapeptide is a superior substrate, with a Km value of 1 X 10(-4) mol/l. The activity of the LD-carboxypeptidase was inhibited by D-amino acids and the beta-lactam antibiotic nocardicin A. Ki values of 0.3 and 43 mmol/l were determined for nocardicin A and D-homoserine, respectively. The properties of the purified enzyme correspond to activity I in ether treated cells.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Metz R,Henning S,Hammes WPdoi
10.1007/BF00414732subject
Has Abstractpub_date
1986-03-01 00:00:00pages
181-6issue
2eissn
0302-8933issn
1432-072Xjournal_volume
144pub_type
杂志文章abstract：:An organism from a goldfish aquarium, isolated on barbital medium, was found to be a Gram-positive coccus which divided in alternating planes, often appearing as a doublet or as a tetrad with adjacent sides flattened. It grew well, although slowly, on rich solid medium (LB agar) and in liquid brain-heart infusion at r...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00288712
更新日期：1993-01-01 00:00:00
abstract：:A 55 kilobase (kb) plasmid (pOZS550) in the non-clumping Lactococcus lactis subsp. lactis strain OZS1 carrying genes for lactose metabolism was characterised. A mobilizable cointegrate plasmid which is formed between pOZS550 and pOZS448 carries the necessary information for conjugation and transfer. Cointegrate format...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00248837
更新日期：1990-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0582-8
更新日期：2010-07-01 00:00:00
abstract：:Mangrove sediment-associated bacteria are of significantly important in the field of medicine and pharmaceuticals as new promising sources of biologically active pharmacophores due to the extreme conditions, such as high salt concentration and soil anoxia. The sediment bacteria associated with Acanthus ilicifolius and...
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pub_type： 杂志文章
doi：10.1007/s00203-020-01999-5
更新日期：2021-01-01 00:00:00
abstract：:Stenotrophomonas maltophilia (Sm), with most of the isolates being resistant to multidrugs, is an opportunistic bacterium causing nosocomial infections. In this study, a novel virulent Sm phage, Smp14, was characterized. Electron microscopy showed that Smp14 resembled members of Myoviridae and adsorbed to poles of the...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0238-5
更新日期：2007-08-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245305
更新日期：1993-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00423075
更新日期：1979-03-12 00:00:00
abstract：:Pulicat Lake sediments are often severely polluted with the toxic heavy metal mercury. Several mercury-resistant strains of Bacillus species were isolated from the sediments and all the isolates exhibited broad spectrum resistance (resistance to both organic and inorganic mercuric compounds). Plasmid curing assay show...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0088-6
更新日期：2006-04-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00416970
更新日期：1976-10-11 00:00:00
abstract：:In addition to rhizobia, other non-symbiotic endophytic bacteria also have been simultaneously isolated from the same root nodules. The existence of non-symbiotic endophytic bacteria in leguminous root nodules is a universal phenomenon. The vast majority of studies have detected endophytic bacteria in other plant tiss...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01752-7
更新日期：2020-03-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00303581
更新日期：1994-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00248617
更新日期：1991-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-020-01929-5
更新日期：2020-10-01 00:00:00
abstract：:The biological effects of UV radiation of different wavelengths (UVA, UVB and UVC) were assessed in nine bacterial isolates displaying different UV sensitivities. Biological effects (survival and activity) and molecular markers of oxidative stress [DNA strand breakage (DSB), generation of reactive oxygen species (ROS)...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0847-5
更新日期：2013-01-01 00:00:00
abstract：:The efficiency of hydrolysis of fungal (Fusarium spp.) cell wall and rye root cell wall by crude enzymatic complexes from (42-day-old) cultures of three F. culmorum isolates, a plant growth-promoting rhizosphere isolate (PGPF) DEMFc2, a deleterious rhizosphere isolate (DRMO) DEMFc5, and a pathogenic isolate DEMFc37, a...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0803-4
更新日期：2012-08-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0764-y
更新日期：2005-03-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0301-2
更新日期：2008-02-01 00:00:00
abstract：:The most important tools in killing and overcoming on the microbes and pathogens that cause diseases in medicine and/or in agriculture are the antibiotics. The discovery and synthesis of the microbial natural products or antibiotics has greatly developed genetically and biotechnologically quickly in the last decades. ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02085-6
更新日期：2020-10-19 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100304
更新日期：2001-07-01 00:00:00
abstract：:Representative strains of Gardnerella vaginalis were degraded using both an alkaline and an acid methanolysis and the fatty acid methyl esters released examined by thin-layer and gas chromatography. The profiles obtained were both qualitatively and quantitatively similar and were comprised of straight chain saturated ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425410
更新日期：1984-05-01 00:00:00
abstract：:The set of physiological and metabolic changes occurring immediately after inoculation and during the lag phase is thought to be of vital importance for optimal offset of fermentation. The transcriptional changes taking place during the lag phase after inoculation of a late-respiratory-phase yeast culture into fresh, ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0527-6
更新日期：2003-04-01 00:00:00
abstract：:Flow cytometry (FCM) in conjunction with immunocytochemical-labeling was used to analyze and screen a population of Escherichia coli clones containing a genomic library from the oil-degrading microorganism Acinetobacter calcoaceticus RAG-1 surface antigens. Reconstruction experiments using mixed populations indicated ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00422282
更新日期：1988-01-01 00:00:00
abstract：:The pathway construction and allosteric regulation of phenylalanine and tyrosine biosynthesis was examined in Neisseria gonorrhoeae. A single 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase enzyme sensitive to feedback inhibition by L-phenylalanine was found. Chorismate mutase and prephenate dehydratase ap...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425071
更新日期：1987-01-01 00:00:00
abstract：:This study describes two Bradyrhizobium strains, UFLA03-164T and UFLA03-153, which share more than 99% sequence similarity of the 16S rRNA with the type strains of 15 species in this genus. The concatenation of three housekeeping genes (recA, gyrB, and dnaK) indicated that both strains formed a single clade separate f...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01827-w
更新日期：2020-07-01 00:00:00
abstract：:We report on the genome-wide response, based on DNA microarrays, of the cyanobacterium Synechocystis sp. PCC 6803 wild type and DeltasigB to a 15 min heat shock. Approximately 9% of the genes in wild type and DeltasigB were significantly regulated (P < 0.001) following this treatment, with chaperones induced the most....
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0138-0
更新日期：2006-10-01 00:00:00
abstract：:Klebsiella pneumoniae NCTC 418 was cultured aerobically in chemostat cultures (D = 0.3 h-1; 35 degrees C) under respectively carbon-, phosphate-, potassium-, sulphate-, and ammonia-limited conditions with glucose as the sole carbon and energy source. The effect of the external pH value on glucose metabolism and on the...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413140
更新日期：1989-01-01 00:00:00
abstract：:The role of the genomic bipyrimidine nucleotide frequency in pyrimidine dimer formation caused by germicidal UV radiation was studied in three microbial reference organisms (Escherichia coli K12, Deinococcus radiodurans R1, spores and cells of Bacillus subtilis 168). The sensitive HPLC tandem mass spectrometry assay w...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0579-3
更新日期：2010-07-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1191-y
更新日期：2016-05-01 00:00:00
abstract：:The family Chlamydiaceae contains nine species pathogenic to humans and animals, but their routine identification is hampered by inadequate detection methods. In an attempt to find a new region for PCR detection and discrimination of the Chlamydiaceae species, the 3' end of the omp2 gene of Chlamydiaceae has been exam...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0757-2
更新日期：2005-03-01 00:00:00
abstract：:In studies on the membranlytic action of various saponins on mycelium of Botrytis cinerea and Rhizoctonia solani digitonin, alpha-hederin and tomatin caused considerable leakage of free amino acids, while aescin and theasaponin were less effective. Cyclamin significantly damaged cell membranes of R. solani, but did no...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447315
更新日期：1975-06-22 00:00:00
