Abstract:
:The nitrite oxidoreductase (NOR) from the facultative nitrite-oxidizing bacterium Nitrobacter hamburgensis X14 was investigated genetically. In order to develop a probe for the gene norB, the N-terminal amino acid sequence of the NOR beta-subunit (NorB) was determined. Based on that amino acid sequence, an oligonucleotide was derived that was used for the identification and cloning of gene norB. Sequence analysis of DNA fragments revealed three adjacent open reading frames in the order norA, norX, norB. The DNA sequences of norX and norB represented complete genes while the open reading frame of norA was truncated by the cloning site. The deduced amino acid sequence of protein NorB contained four cysteine clusters with striking homology to those of iron-sulfur centers of bacterial ferredoxins. NorB shares significant sequence similarity to the beta-subunits (NarH, NarY) of the two dissimilatory nitrate reductases (NRA, NRZ) of Escherichia coli. Additionally, the derived amino acid sequence of the truncated open reading frame of norA showed striking resemblance to the alpha-subunits (NarG, NarZ) of the E. coli nitrate reductases.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Kirstein K,Bock Edoi
10.1007/BF00245305subject
Has Abstractpub_date
1993-01-01 00:00:00pages
447-53issue
6eissn
0302-8933issn
1432-072Xjournal_volume
160pub_type
杂志文章abstract::Hyphal development in Candida albicans was blocked by EDTA. This effect was not due to a general growth inhibition since the chelator did not affect protein and DNA synthesis. Recovery of mycelial growth was observed when EDTA-grown cells were incubated at 37 degrees C in EDTA-free medium. High-molecular-weight mannop...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00307769
更新日期:1994-01-01 00:00:00
abstract::The thermotolerant methylotroph Bacillus sp. C1 possesses a novel NAD-dependent methanol dehydrogenase (MDH), with distinct structural and mechanistic properties. During growth on methanol and ethanol, MDH was responsible for the oxidation of both these substrates. MDH activity in cells grown on methanol or glucose wa...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00245161
更新日期:1992-01-01 00:00:00
abstract::The diversity of eukaryotic populations, in particular protozoa, in the water supplies of intensively reared broilers has not been previously studied. This important food-rearing environment was screened for the molecular diversity of eukaryotes by the analysis of PCR-amplified 18S rRNA. DNA was extracted from filtere...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-006-0119-3
更新日期:2006-07-01 00:00:00
abstract::Ever since the development of the first antibiotic compound with anticancer potential, researchers focused on isolation and characterization of prospective microbial natural products with potential anti-infective and anticancer activities. The present work describes the production of bioactive metabolites by heterotro...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02106-4
更新日期:2020-11-02 00:00:00
abstract::An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 degrees C and 5.4 g NaCl l(-1). The strain grows heterotrophically on complex pr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050745
更新日期:1999-08-01 00:00:00
abstract::Azotobacter vinelandii was grown in oxygen-controlled continuous cultures under conditions of dinitrogen fixation. Different oxygen concentrations were adjusted with air. Cell-free extracts were employed to study the oxygen dependency of the intracellular distribution and activity of the following enzymes: nitrogenase...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00415614
更新日期:1983-10-01 00:00:00
abstract::Glucose-limited continuous cultures were used to analyze sigmaB activity at decreasing growth rates. Expression of the sigmaB-dependent genes gsiB and ctc started to increase at a growth rate of 0.2 h-1, and both genes were induced approximately fivefold at a growth rate of 0.1 h-1 as compared to expression at the max...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050731
更新日期:1999-05-01 00:00:00
abstract::Bacillus subtilis strain 168 encodes two flavocytochromes P450, Cyp102A2 and Cyp102A3. The cyp102A3 gene is preceded by, and organized in an operon with, a gene for a transcriptional regulator, encoded by fatR. The paralogous gene, cyp102A2, is most likely transcribed as a mono-cistronic message. We show that fatR enc...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030100350
更新日期:2001-12-01 00:00:00
abstract::The main property of an Amt- (ammonium transport negative) mutant of Klebsiella pneumoniae is its inability to accumulate NH4+ intracellularly. When growing on nitrogen sources other than NH4+, the mutant constantly looses NH3 by diffusion. This loss results in poor growth. The NH3 excretion suggests the existence of ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00402008
更新日期:1984-10-01 00:00:00
abstract::Mercury resistance of Clostridium cochlearium T-2P was found to be controlled by a different mechanism from those reported so far since no mercury-reducing activity was detected in this strain. The H2S generating ability as well as the demethylating activity in this bacterium was eliminated by the treatment of the cur...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00417179
更新日期:1981-03-01 00:00:00
abstract::Acinetobacter calcoaceticus LMD 79.41 produced significant amounts of pyrrolo-quinoline quinone (PQQ) in its culture medium when grown on quinic acid or shikimic acid. Studies with LMD 79.41 and PQQ- -mutants of this strain demonstrated that this organism contains an NAD(P)-independent quinate dehydrogenase (QDH) (EC ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00409714
更新日期:1988-05-01 00:00:00
abstract::The isolated cell wall of Streptomyces griseus 52-1 strain labelled with fluorescein isothiocyanate (FITC) and containing wall-bound autolytic enzyme was lysed as a function of different cations. The autolysis was accelerated by aminoglycoside antibiotics (streptomycin and the structurally closely related neomycin) wh...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00291282
更新日期:1990-01-01 00:00:00
abstract::The diversity among 45 cyanobacterial isolates from 11 different Gunnera species originating from different geographical areas was examined. By means of polymerase chain reaction (PCR) fingerprinting with short tandemly repeated repetitive (STRR) sequences as primers, ten groups of symbiotic cyanobacteria and five uni...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002039900113
更新日期:2000-02-01 00:00:00
abstract::Light-limited batch cultures of Synechococcus spp. strains PCC 6301 and WH 8103 exhibited a positive correlation between the specific growth rate and the cellular content of both RNA and DNA. The ratio of RNA to DNA increased with the growth rate in Synechococcus sp. strain WH 8103, as it does in heterotrophic bacteri...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050634
更新日期:1998-09-01 00:00:00
abstract::The marine bacterium Vibrio psychoerythrus, an obligate psychrophile, contains uniformly dense rod-shaped organelles 10-15 nm wide and up to 1.3 mum long. These structures were frequently seen crossing the septum of dividing cells. ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00427869
更新日期:1976-02-01 00:00:00
abstract::Two genes, aphE and orf, coding for putative Mr 29,000 and Mr 31,000, proteins respectively, were identified in the nucleotide sequence of a 2.8 kbp DNA segment cloned from Streptomyces griseus N2-3-11. The aphE gene expressed streptomycin (SM) resistance and a SM phosphorylating enzyme in S. lividans strains. The two...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425160
更新日期:1988-01-01 00:00:00
abstract::Representative strains of Gardnerella vaginalis were degraded using both an alkaline and an acid methanolysis and the fatty acid methyl esters released examined by thin-layer and gas chromatography. The profiles obtained were both qualitatively and quantitatively similar and were comprised of straight chain saturated ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425410
更新日期:1984-05-01 00:00:00
abstract::It has been shown previously that externally added glycine betaine is accumulated in Escherichia coli in response to the external osmotic strength. Here we have shown, by using nuclear magnetic resonance spectroscopy and radiochemical methods, that E. coli growing in a glucose-mineral medium of elevated osmotic streng...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00492896
更新日期:1987-02-01 00:00:00
abstract::Plasmid-borne hup-lacZ transcriptional fusion constructs were introduced into three separate mutant strains of Bradyrhizobium japonicum which express hydrogenase constitutively (Hupc strains SR470, SR473 and JH101) in both autotrophic and heterotrophic environments. The lacZ structural gene linked directly to the regu...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00258144
更新日期:1993-01-01 00:00:00
abstract::The twin-arginine translocation (Tat) system serves to export fully folded proteins across the cytoplasmic membrane. In many bacteria, three major components, TatA, TatB and TatC, are the functionally essential constituents of the Tat system. A Myxococcus xanthus tatB-tatC deletion mutant could aggregate and form moun...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0067-3
更新日期:2006-02-01 00:00:00
abstract::The polymorphic GC-rich repetitive sequence (PGRS) found on the chromosome of Mycobacterium tuberculosis was characterized by means of mapping, cloning and sequencing. PGRS was present in at least 26 loci and consisted of many tandem repeats of the consensus sequence CGGCGGCAA. As the core of the consensus motif was t...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00381781
更新日期:1995-02-01 00:00:00
abstract::We previously constructed an arabinose-inducible recombinant Bifidobacterium longum that could efficiently express secreted IFN-alpha2b in vitro (Deng et al. in Arch Microbiol 191:681-686, 2009). Here, we investigated the influence of oral pBAD-SPIFN-transformed B. longum on immunomodulation and IFN-induced Mx1 gene t...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-010-0589-1
更新日期:2010-08-01 00:00:00
abstract::The alpha-glucosidase (alpha-D-glucoside glucohydrolase, EC 3.2.1.20) of Pseudomonas fluorescens W was partially purified by (NH4)2SO4 fractionation, Sephadex G-200 and DEAE-cellulose column chromatography. The enzyme showed great specificity for maltose hydrolysis, with very little action against polymeric forms. Suc...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425338
更新日期:1976-04-01 00:00:00
abstract::BvgAS is a two-component system of Bordetella pertussis involved in the reciprocal regulation of the virulence genes and the flagellar biosynthesis. In this study, we found that expression of bvgAS in Escherichia coli also results in reduced motility. The repression was relieved by the addition of known chemical modul...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050688
更新日期:1999-01-01 00:00:00
abstract::The regulation of the syntheses of two arabinan-degrading extracellular enzymes and several intracellular L-arabinose catabolic enzymes was examined in wild-type and carbon catabolite derepressed mutants of Aspergillus nidulans. alpha-L-Arabinofuranosidase B, endoarabinase, L-arabinose reductase, L-arabitol dehydrogen...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00282109
更新日期:1994-01-01 00:00:00
abstract::Stress resistance and fermentative capability are important quality characteristics of baker's yeast. In the present study, we examined protective effects of exogenous alpha-ketoglutarate (AKG), an intermediate of the tricarboxylic acid cycle and amino acid metabolism, against freeze-thaw and carbohydrate-induced stre...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-017-1423-9
更新日期:2018-01-01 00:00:00
abstract::The spectroscopic properties of stigma inside green and dark-grown cells and of isolated stigma globules have been studied by means of a microspectrophotometer build in the Laboratory. On the base of these results and of the analysis of the absorption spectra of a stigma suspension, cell suspension and the cell methan...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446551
更新日期:1976-12-01 00:00:00
abstract::DNA acquisition promotes the spread of resistance to antibiotics and virulence among bacteria. It is also linked to several natural phenomena including recombination, genome dynamics, adaptation and speciation. Horizontal DNA transfer between bacteria occurs via conjugation, transduction or competence for natural tran...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-013-0870-1
更新日期:2013-04-01 00:00:00
abstract::An organism from a goldfish aquarium, isolated on barbital medium, was found to be a Gram-positive coccus which divided in alternating planes, often appearing as a doublet or as a tetrad with adjacent sides flattened. It grew well, although slowly, on rich solid medium (LB agar) and in liquid brain-heart infusion at r...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00288712
更新日期:1993-01-01 00:00:00
abstract::The ability of soil microorganisms to solubilize phosphate is an important trait of plant growth-promoting bacteria leading to increased yields and smaller use of fertilizers. This study presents the isolation and characterization of phosphobacteria from Puna, northwestern Argentina and the ability to produce phosphat...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-011-0692-y
更新日期:2011-07-01 00:00:00