Understanding the host-adapted state of Citrobacter rodentium by transcriptomic analysis.

Abstract:

:Citrobacter rodentium (Cr) is a mouse pathogen that mimics many aspects of enteropathogenic Escherichia coli infections including producing attaching and effacing (A/E) lesions. Host-adapted (HA) Cr cells that are shed at the peak of infection have been reported to be hyper-infective. The exact mechanism underlying this phenomenon has remained elusive since the pathogen loses its HA 'status' immediately upon subculturing in laboratory media. We sequenced the entire transcriptome of Cr directly from the feces of infected mice and analyzed the gene expression pattern. We observed that the entire transcriptional machinery as well as several transcriptional regulators to be differentially expressed when compared with the transcriptome of cells grown on laboratory media. Major adhesion and effector genes, tir and eae, were highly expressed in HA along with many genes located on all five loci of enterocyte effacement regions (LEE 1-5). Notable absent among the HA expressed genes were 19 fimbrial operons and non-fimbrial adhesions and several non-LEE encoded effectors. These results demonstrate that host-adapted Cr has a unique transcriptome that is associated with increased host transmission.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Smith AD,Yan X,Chen C,Dawson HD,Bhagwat AA

doi

10.1007/s00203-016-1191-y

subject

Has Abstract

pub_date

2016-05-01 00:00:00

pages

353-62

issue

4

eissn

0302-8933

issn

1432-072X

pii

10.1007/s00203-016-1191-y

journal_volume

198

pub_type

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