Abstract:
:To identify virulence-associated genes of a fish pathogen Yersinia ruckeri, we screened a total of 1056 mini-Tn5-Km2 signature-tagged mutants in rainbow trout by immersion challenge. Of 1056, 25 mutants were found survival-defective as they could not be re-isolated from fish kidney 7 days after infection. Mutated gene in F2-4 mutant, one of the 25 mutants, was homologous to uvrY that encodes UvrY response regulator of BarA-UvrY two-component system (TCS). Mutant F2-4 was significantly more sensitive (P < 0.05) to H2O2-mediated killing and was less able to infect Epithelioma papulosum cyprini cells. However, UvrY mutation did not affect survival of F2-4 mutant in the presence of non-immune fish serum and its ability to grow under iron starvation. In a time-course co-infection, mutant F2-4 had lower bacterial loads on day 1 itself, and by day 5 there was nearly a 1,000-fold difference in infection levels of the parent and mutant strains. The barA homolog of Y. ruckeri was PCR-amplified and sequence analyses identified four domains that were characteristic of hybrid histidine kinases. To conclude, the BarA-UvrY TCS contributes to the pathogenesis of Y. ruckeri in its natural host rainbow trout, possibly by regulating invasion of epithelial cells and sensitivity to oxidative stress induced by immune cells.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Dahiya I,Stevenson RMdoi
10.1007/s00203-010-0582-8subject
Has Abstractpub_date
2010-07-01 00:00:00pages
541-7issue
7eissn
0302-8933issn
1432-072Xjournal_volume
192pub_type
杂志文章abstract::Grasses of the Urochloa genus have been widely used in crop-livestock integration systems or as cover crops in no-till systems such as in rotation with maize. Some species of Urochloa have mechanisms to reduce nitrification. However, the responses of microbial functions in crop-rotation systems with grasses and its co...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01910-2
更新日期:2020-09-01 00:00:00
abstract::The metabolism of methylamine as the nitrogen source for growth of the non-methylotrophic yeast Candida utilis and the methylotrophic yeast Hansenula polymorpha was investigated. Growth of both organisms in media with glucose and methylamine was associated with the presence of an amine oxidase in these cells. The enzy...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00511216
更新日期:1980-06-01 00:00:00
abstract::Mycothiol (MSH) plays important roles in maintaining cytosolic redox homeostasis and in adapting to reactive oxygen species in the high-(G + C)-content Gram-positive Actinobacteria. However, its physiological roles are ill defined compared to glutathione, the functional analog of MSH in Gram-negative bacteria and most...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-013-0889-3
更新日期:2013-06-01 00:00:00
abstract::The signal orchestration between legumes and the rhizobia attribute to symbiotic nitrogen fixation through nodule formation. Root nodules serve as a nutrient-rich reservoir and harbor diverse microbial communities. However, the existence of non-rhizobial endophytes (NRE) and their role inside the root nodules are bein...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01983-z
更新日期:2020-12-01 00:00:00
abstract::A Gram-stain-negative, strictly aerobic, beige-pigmented, motile, chemoheterotrophic, rod-shaped or ovoid bacterium, designated strain KMU-37T, was isolated from seawater at Najeong Beach in the Republic of Korea. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1329-y
更新日期:2017-05-01 00:00:00
abstract::The present study investigated the endophytic bacterial communities in the seeds of mature, natural common reed (Phragmites australis) and narrowleaf cattail (Typha angustifolia L.). Additionally, seed endophytic bacterial communities were compared with rhizospheric and root endophytic bacterial communities using Illu...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-018-1493-3
更新日期:2018-08-01 00:00:00
abstract::Beta-Lactamase (penicillinase) activity was found in a number of strains of blue-green algea. In some cases, this enzyme permitted algae to overcome the inhibitory effects of penicillin. Production and localization of beta-lactamase were studied in a unicellular species, Coccochloris elabens (strain 7003), and in a fi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00429338
更新日期:1977-03-01 00:00:00
abstract::Listeria monocytogenes was examined for the presence of surface carbohydrates to ascertain whether surface sugars, if present, would interact with eucaryotic surface carbohydrate receptors. We found that a virulent, but not two avirulent strains had a surface alpha-D-galactose residue as determined by agglutination wi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00249083
更新日期:1990-01-01 00:00:00
abstract::Coagulase-negative staphylococci (CNS) are opportunistic pathogens that are currently emerging as causative agents of human disease. Though CNS are widespread in the clinic and food, their precise identification at species level is important. Here, using 16S rRNA sequencing, 55 staphylococcal isolates were identified ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-017-1415-9
更新日期:2018-01-01 00:00:00
abstract::Bacteria in Lactobacillus casei group, including Lactobacillus casei (L. casei), Lactobacillus paracasei (L. paracasei), and Lactobacillus rhamnosus (L. rhamnosus) are important lactic acid bacteria in the production of fermented dairy products and are faced with the controversial nomenclatural status due to their clo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-018-1528-9
更新日期:2018-09-01 00:00:00
abstract::Pure cultures of an aerobically grown Flavobacterium sp. were shown by hydride generation-cold trap-atomic absorption spectrometry to biomethylate inorganic antimony (III) supplied as potassium antimony tartrate. Growth inhibition of the Flavobacterium sp. by antimony (III) over the range 0-30 mg Sb l(-1) was assessed...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0456-9
更新日期:2002-10-01 00:00:00
abstract::The distribution of sex pheromone induced aggregation substance was studied on the cell surface of various Enterococcus faecalis strains. In the accompanying paper we have shown that the aggregation substance appears as a layer of hairlike structures. Using direct and indirect immunogold technique, transmission electr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00454864
更新日期:1989-01-01 00:00:00
abstract::The carboxysomal polypeptides of Thiobacillus neapolitanus with apparent molecular masses of 85 and 130 kDa were isolated and subjected to N-terminal sequencing. The first 17 amino acids of the two peptides were identical. The sequence perfectly matched the deduced amino acid sequence of an open reading frame in the c...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050765
更新日期:1999-10-01 00:00:00
abstract::Rhizopus oryzae has long been used for enzyme production (e.g., glucoamylase and lipase), organic acid synthesis, and various fermented food applications. In this work, we describe a set of plasmid-based expression vectors that can be used for the production of heterologous proteins in R. oryzae. Three plasmid vectors...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-006-0121-9
更新日期:2006-07-01 00:00:00
abstract::A novel actinomycete, strain PA1-10T, isolated from the leaf of Phyllanthus amarus collected from Bangkok, Thailand, was characterized taxonomically using a polyphasic approach. This strain contained the characteristics consistent with those of members of the genus Nonomuraea. It formed short rugose spore chain on aer...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01717-w
更新日期:2020-01-01 00:00:00
abstract::The sphere-rod-sphere morphology cycle of Arthrobacter crystallopoietes was accompanied by changes in the rate of growth and the rates of DNA, RNA and protein synthesis. The patterns of macromolecule synthesis resembled those found in other bacteria during a step-up followed by a step-down in growth rate. During the s...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446549
更新日期:1976-12-01 00:00:00
abstract::Organic acid production including lactate and acetate is an economically attractive technology that has gained momentum worldwide over the past years. These series of action need to be performed by an esoteric and complex microbial community, in which different members have distinct roles in the establishment of a col...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01676-2
更新日期:2019-11-01 00:00:00
abstract::Calcium is essential for the growth of rhizobia and the formation of nitrogen-fixing root-nodules on legumes, but its precise role in these processes remains unknown. We have found that Rhizobium fredii USDA208 accumulates a major 38 kDa protein when grown in media supplemented with 0.3-2 microM Cacl2. We have purifie...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00248480
更新日期:1993-01-01 00:00:00
abstract::A gram-negative bacterium designated BN30(T), which is motile with a polar flagellum, non-endospores forming, oxidase- and catalase-positive, was isolated from soybean root nodule. The organism is facultative anaerobic and surface-wrinkled rod. It can grow at 10-40 °C, pH 6-8 and 6 % (w/v) NaCl. BLASTn search based on...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-015-1102-7
更新日期:2015-06-01 00:00:00
abstract::Saccharomyces cerevisiae mutants unable to grow at ethanol concentrations at which the wild type strain S288C does grow, have been isolated. Some of them show additional phenotypic alterations in colony size, temperature sensitivity and viability in ethanol, which cosegregate with the growth sensitivity in ethanol. 21...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00412799
更新日期:1986-01-01 00:00:00
abstract::The spectroscopic properties of stigma inside green and dark-grown cells and of isolated stigma globules have been studied by means of a microspectrophotometer build in the Laboratory. On the base of these results and of the analysis of the absorption spectra of a stigma suspension, cell suspension and the cell methan...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446551
更新日期:1976-12-01 00:00:00
abstract::Two bacterial isolates from Great Bay Estuary, New Hampshire, in co-culture carried out anaerobic dissimilation of citric acid with Fe(III) as the terminal electron acceptor. Neither isolate oxidized citrate with Fe(III) anaerobically in axenic culture. The Fe(III) reducer, Shewanella alga strain BrY, did not grow ana...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050383
更新日期:1996-10-17 00:00:00
abstract::Chromate resistance in Ralstonia metallidurans CH34 is based on chromate efflux catalyzed by ChrA efflux pumps. The bacterium harbors two chromate resistance determinants, the previously known chr(1) on plasmid pMOL28 (genes chrI, chrB(1), chrA(1), chrC, chrE, chrF(1)) and chr(2) on the chromosome (genes chrB(2), chrA...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0492-5
更新日期:2002-12-01 00:00:00
abstract::VcrD1 protein is a component of type III secretion system (T3SS) 1 in Vibrio parahaemolyticus. A comparative analysis of secretomes of wild-type and ΔvcrD1 strains revealed that the mutant was defective in secretion of diverse proteins including several flagellar components. Western blot analyses using specific antibo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-1069-9
更新日期:2015-04-01 00:00:00
abstract::Only about one third of humans possess a microbiota capable of transforming the dietary isoflavone daidzein into equol. Little is known about the dietary and physiological factors determining this ecological feature. In this study, the in vitro metabolism of daidzein by faecal samples from four human individuals was i...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0747-4
更新日期:2005-01-01 00:00:00
abstract::It was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus Frankia. Recently, the number of nitrogen fixation (nifH) genes identified in other non-Frankia actinobacteria has dramatically increased and has open...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-011-0733-6
更新日期:2012-01-01 00:00:00
abstract::Recently, we reported the synthesis of five bacteriocin-like inhibitor substances (Bt-BLIS: morricin 269, kurstacin 287, kenyacin 404, entomocin 420, and tolworthcin 524) by Mexican strains of Bacillus thuringiensis. Here we show that, collectively, these Bt-BLIS have a moderate to broad spectrum of antibacterial acti...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-008-0414-2
更新日期:2008-12-01 00:00:00
abstract::In order to investigate the role of the putative epimerase function of the beta-oxidation multienzyme complex (FadBA) in the provision of (R)-3-hydroxyacyl-CoA thioesters for medium-chain-length polyhydroxyalkanoate (PHA(MCL)) biosynthesis, the fadBA(Po) operon of Pseudomonas oleovorans was cloned and characterized. T...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0444-0
更新日期:2002-08-01 00:00:00
abstract::Some unicellular N(2)-fixing cyanobacteria have recently been found to lack a functional photosystem II of photosynthesis. Such organisms, provisionally termed UCYN-A, of the oceanic picoplanktion are major contributors to the global marine N-input by N(2)-fixation. Since their photosystem II is inactive, they can per...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-010-0621-5
更新日期:2010-10-01 00:00:00
abstract::Respiratory NADH oxidation in the rumen bacterium Prevotella bryantii is catalyzed by the Na+-translocating NADH:quinone oxidoreductase (NQR). A method for cell disruption and membrane isolation of P. bryantii under anoxic conditions using the EmulisFlex-C3 homogenizer is described. We compared NQR activity and protei...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01805-x
更新日期:2020-07-01 00:00:00