Ethanol-sensitive mutants of Saccharomyces cerevisiae.

Abstract:

:Saccharomyces cerevisiae mutants unable to grow at ethanol concentrations at which the wild type strain S288C does grow, have been isolated. Some of them show additional phenotypic alterations in colony size, temperature sensitivity and viability in ethanol, which cosegregate with the growth sensitivity in ethanol. 21 selected monogenic ethanol-sensitive mutants define 20 complementation groups, denominated ETA1 to ETA20, which indicates that there is a high number of genes involved in the ethanol tolerance/sensitivity mechanism. Out of 21 selected monogenic mutants, 20 are not altered in the glycolytic pathway since, when maintained in glucose-supplemented medium, they can produce as much ethanol as the wild type and at about the same velocity. Nor do any of the mutants seem to be altered in the lipid biosynthetic pathway since, whether grown in the absence or in the presence of ethanol, their concentration of fatty acids and ergosterol is similar to that of the wild type under the same conditions. Therefore growth sensitivity to ethanol does not seem necessarily to be related to carbohydrate or lipid metabolism.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Aguilera A,Benítez T

doi

10.1007/BF00412799

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

337-44

issue

4

eissn

0302-8933

issn

1432-072X

journal_volume

143

pub_type

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