Abstract：
:Coagulase-negative staphylococci (CNS) are opportunistic pathogens that are currently emerging as causative agents of human disease. Though CNS are widespread in the clinic and food, their precise identification at species level is important. Here, using 16S rRNA sequencing, 55 staphylococcal isolates were identified as S. capitis, S. caprae, S. epidermidis, S. haemolyticus, S. pasteuri, S. saprophyticus, S. warneri, and S. xylosus. Although 16S rRNA sequencing is universally accepted as a standard for bacterial identification, the method did not effectively discriminate closely related species, and additional DNA sequencing was required. The divergence of the sodA gene sequence is higher than that of 16S rRNA. To devise a rapid and accurate identification method, sodA-specific primers were designed to demonstrate that species-specific multiplex polymerase chain reaction (PCR) can be used for the identification of CNS species. The accuracy of this method was higher than that of phenotypic identification; the method is simple and less time-consuming than 16S rRNA sequencing. Of the 55 CNS isolates, 92.72% were resistant to at least one antibiotic, and 60% were resistant to three or more antibiotics. CNS isolates produced diverse virulence-associated enzymes, including hemolysin (produced by 69.09% of the isolates), protease (65.45%), lipase (54.54%), lecithinase (36.36%), and DNase (29.09%); all isolates could form a biofilm. Because of the increasing pathogenic significance of CNS, the efficient multiplex PCR detection method developed in this study may contribute to studies for human health.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Kim J,Hong J,Lim JA,Heu S,Roh Edoi
10.1007/s00203-017-1415-9subject
Has Abstractpub_date
2018-01-01 00:00:00pages
73-83issue
1eissn
0302-8933issn
1432-072Xpii
10.1007/s00203-017-1415-9journal_volume
200pub_type
杂志文章abstract：:The gram-negative bacterium Myxobacter sp. AL-1 produces chitosanase-cellulase activity that is maximally excreted during the stationary phase of growth. Carboxymethylcellulase zymogram analysis revealed that the enzymatic activity was correlated with two bands of 32 and 35 kDa. Ion-exchange-chromatography-enriched pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
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journal_title：Archives of microbiology
pub_type： 杂志文章
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journal_title：Archives of microbiology
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doi：10.1007/s00203-006-0112-x
更新日期：2006-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-12-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1986-11-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425410
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446551
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425066
更新日期：1979-06-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1103-6
更新日期：2015-06-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0440-0
更新日期：2009-02-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0687-8
更新日期：2011-06-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0911-9
更新日期：2013-09-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00421893
更新日期：1980-05-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00404204
更新日期：1995-05-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0589-1
更新日期：2010-08-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-020-02041-4
更新日期：2020-09-24 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0533-4
更新日期：2010-02-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0808-z
更新日期：2012-09-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447338
更新日期：1975-08-28 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00414437
更新日期：1989-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00521289
更新日期：1982-12-03 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s002030050484
更新日期：1997-09-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-020-01929-5
更新日期：2020-10-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447319
更新日期：1975-06-22 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0638-0
更新日期：2004-02-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00427842
更新日期：1977-10-24 00:00:00
abstract：:CO2 is required continuously during germination of Streptomyces viridochromogenes spores. Spores incubated in a defined germination medium in the absence of CO2 remain phase bright and do not release spore carbon. In the presence of CO2, the spores initiate germination accompanied by loss of refractility and spore car...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429118
更新日期：1978-09-01 00:00:00
abstract：:The proteins in the culture supernatant (exoproteins) from Streptococcus pyogenes serotype M1 were separated by two-dimensional gel electrophoresis, and their N-terminal amino acid sequences were determined. The amino acid sequences were compared to sequences in the S. pyogenes genome database. The coding sequence sho...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0412-8
更新日期：2002-06-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-020-01862-7
更新日期：2020-08-01 00:00:00
abstract：:Comparative 16S rRNA gene sequence and genomic DNA reassociation analyses were used to assess the phylogenetic relationships of Methanobrevibacter fecal isolates. The 16S rRNA gene sequences of Methanobrevibacter smithii strain PS and the human fecal isolates B181 and ALI were essentially identical, and their genomic ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050589
更新日期：1998-05-01 00:00:00
