Properties of the cell envelope and a cell-envelope protein of Pseudomonas facilis.

Abstract:

:The molecular weight of the protein moiety of a phospholipoprotein complex isolated from Pseudomonas facilis has been examined with a variety of sodium dodecylsulfate-polyacrylamide gel electrophoretic systems. A molecular weight of 35 000 was determined for the protein in all analyses. A 35 000-dalton protein was present in the EDTA extract of P. facilis and in the cytoplasmic and outer membrane fractions, but not in the lipopolysaccharide and peptidoglycan. Prior inoculation of mice with the phospholipoprotein complex led to a 7.5- to 15-fold increase in the LD50 when mice were subsequently inoculated with Salmonella typhimurium; this pathogen has a cell-surface protein which cross-reacts immunologically with antibody to the P. facilis phospholipoprotein complex.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Jones RC,McFadden BA

doi

10.1007/BF00427842

subject

Has Abstract

pub_date

1977-10-24 00:00:00

pages

37-43

issue

1

eissn

0302-8933

issn

1432-072X

journal_volume

115

pub_type

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