Oxidation of formaldehyde by alcohol oxidase of Candida boidinii.

Abstract:

:A fromaldehyde oxidase activity was found in cellfree extracts of methanol-grown yeast Candida boidinii. Loss of alcohol oxidase activity in a mutant, 48, led to loss of the formaldehyde oxidase activity, indicating that the same enzyme is probably responsible for both activities. This could be demonstrated with the purified alcohol oxidase which oxidizes, besides lower primary alcohols, formaldehyde to formate. The Km value for formaldehyde is 5.7 mM. It seems that alcohol oxidase is not implicated in formaldehyde oxidation in vivo.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Sahm H

doi

10.1007/BF00447134

keywords:

subject

Has Abstract

pub_date

1975-10-27 00:00:00

pages

179-81

issue

2

eissn

0302-8933

issn

1432-072X

journal_volume

105

pub_type

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