Characterization and complete genome analysis of Bacillus velezensis CB6 revealed ATP synthase subunit α against foodborne pathogens.

Abstract:

:Given the serious threat of foodborne multidrug-resistant bacteria to animals and humans, finding an effective antibacterial compound has always been an important topic for scientists. Here, from the soil of Changbaishan, we have identified a bacterium that can inhibit the growth of Staphylococcus aureus. Nr genome database analysis and phylogenetic analysis showed that strain CB6 belongs to Bacillus velezensis. We found that the crude extract of strain CB6 has broad-spectrum antibacterial activity against foodborne pathogens. In addition, we showed that the crude extract loses antibacterial activity after treatment with papain. Next, strain CB6 was purified using ammonium sulfate precipitation, a Sephadex G-75 gel filtration column and high-performance liquid chromatography system (HPLC). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis indicated that the antibacterial compound was the protein ATP synthase subunit α (ATP-1), with a molecular weight of 55.397 KDa. Moreover, we reported the complete genome sequence of strain CB6, which is composed of a unique circular 3,963,507 bp chromosome with 3749 coding genes and a G + C content of 46.53%. The genome contained 12 gene clusters with antibacterial functions, which constituted over 20.947% of the complete genome. Of note, the amino acid sequence encoding the ATP-1 protein in the strain CB6 genome was identified. In addition to these findings, we speculate that the ATP-1 protein may provide energy for secondary metabolites, which in turn will improve the antibacterial activity of the secondary metabolites. All the above important features make the ATP-1 as a potential candidate for the development of new antibacterial drugs and food preservatives in the future.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Zhang H,Xu GY,Mao C,Xu Y,Dong W,Inam M,Pan S,Hu R,Wang Y,Wang Y,Ma H,Kong L

doi

10.1007/s00203-020-02102-8

subject

Has Abstract

pub_date

2020-11-04 00:00:00

eissn

0302-8933

issn

1432-072X

pii

10.1007/s00203-020-02102-8

pub_type

杂志文章
  • The response of Serratia marcescens JG to environmental changes by quorum sensing system.

    abstract::Many bacterial cells are known to regulate their cooperative behaviors and physiological processes through a molecular mechanism called quorum sensing. Quorum sensing in Serratia marcescens JG is mediated by the synthesis of autoinducer 2 (AI-2) which is a furanosyl borate diester. In this study, the response of quoru...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-016-1213-9

    authors: Sun SJ,Liu HJ,Weng CH,Lai CF,Ai LY,Liu YC,Zhu H

    更新日期:2016-08-01 00:00:00

  • Insights into the mode of action of anticandidal herbal monoterpenoid geraniol reveal disruption of multiple MDR mechanisms and virulence attributes in Candida albicans.

    abstract::The anticandidal potential of Geraniol (Ger) against Candida albicans has already been established. The present study reveals deeper insights into the mechanisms of action of Ger. We observed that the repertoire of antifungal activity was not only limited to C. albicans and its clinical isolates but also against non-a...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-016-1205-9

    authors: Singh S,Fatima Z,Hameed S

    更新日期:2016-07-01 00:00:00

  • Environmental regulation of alcohol metabolism in thermotolerant methylotrophic Bacillus strains.

    abstract::The thermotolerant methylotroph Bacillus sp. C1 possesses a novel NAD-dependent methanol dehydrogenase (MDH), with distinct structural and mechanistic properties. During growth on methanol and ethanol, MDH was responsible for the oxidation of both these substrates. MDH activity in cells grown on methanol or glucose wa...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00245161

    authors: Arfman N,de Vries KJ,Moezelaar HR,Attwood MM,Robinson GK,van Geel M,Dijkhuizen L

    更新日期:1992-01-01 00:00:00

  • Fish borne Edwardsiella tarda eha involved in the bacterial biofilm formation, hemolytic activity, adhesion capability and pathogenicity.

    abstract::Edwardsiella tarda (E. tarda) is distributed widely in a variety of hosts including humans, other mammals and fish, and it is worthwhile to notice that E. tarda -caused fish infections lead to the most important bacterial disease in fish. Considering Eha acting as a transcriptional regulator in E. tarda strain ET13 ha...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-019-01794-x

    authors: Hassan HA,Ding X,Zhang X,Zhu G

    更新日期:2020-05-01 00:00:00

  • An ammonia-oxidizing bacterium, Nitrosovibrio tenuis nov. gen. nov. sp.

    abstract::An ammonia-oxidizing, autotroph growing, slender, curved rod was isolated from the soil of Hawaii. It is well distinguishable from any other nitrifying bacteria thus far described by their morphology. The cells are 1.1-3.0 mum long and 0.3-0.4 mum wide. They are motile by means of 1-4 subpolar to lateral flagella. In ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00425099

    authors: Harms H,Koops HP,Wehrmann H

    更新日期:1976-05-03 00:00:00

  • Identification and molecular characterization of twin-arginine translocation system (Tat) in Xanthomonas oryzae pv. oryzae strain PXO99.

    abstract::Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, one of the most widespread and destructive bacterial diseases in rice. This study identified and characterized the contribution of the twin-arginine translocation (Tat) pathway to motility, chemotaxis, extracellular polysaccharide (EPS) production and virulen...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-008-0440-0

    authors: Chen L,Hu B,Qian G,Wang C,Yang W,Han Z,Liu F

    更新日期:2009-02-01 00:00:00

  • Further evidence for the regulation of bacterial populations in soil by protozoa.

    abstract::After the addition to soil of large numbers of a cowpea Rhizobium strain, the population declined steadily until the numbers reached about 10(7)/g, and the protozoa rose to about 10(4)/g. When indigenous protozoa were suppressed by the addition of actidione to the soil, the density of the test rhizobium did not fall i...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00492022

    authors: Habte M,Alexander M

    更新日期:1977-06-20 00:00:00

  • Taxonomic structure and function of seed-inhabiting bacterial microbiota from common reed (Phragmites australis) and narrowleaf cattail (Typha angustifolia L.).

    abstract::The present study investigated the endophytic bacterial communities in the seeds of mature, natural common reed (Phragmites australis) and narrowleaf cattail (Typha angustifolia L.). Additionally, seed endophytic bacterial communities were compared with rhizospheric and root endophytic bacterial communities using Illu...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-018-1493-3

    authors: Gao T,Shi XY

    更新日期:2018-08-01 00:00:00

  • Fatty-acid-displaced transcriptional repressor, a conserved regulator of cytochrome P450 102 transcription in Bacillus species.

    abstract::Bacillus subtilis strain 168 encodes two flavocytochromes P450, Cyp102A2 and Cyp102A3. The cyp102A3 gene is preceded by, and organized in an operon with, a gene for a transcriptional regulator, encoded by fatR. The paralogous gene, cyp102A2, is most likely transcribed as a mono-cistronic message. We show that fatR enc...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030100350

    authors: Gustafsson MC,Palmer CN,Wolf CR,von Wachenfeldt C

    更新日期:2001-12-01 00:00:00

  • Enzymic arrangement and allosteric regulation of the aromatic amino acid pathway in Neisseria gonorrhoeae.

    abstract::The pathway construction and allosteric regulation of phenylalanine and tyrosine biosynthesis was examined in Neisseria gonorrhoeae. A single 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase enzyme sensitive to feedback inhibition by L-phenylalanine was found. Chorismate mutase and prephenate dehydratase ap...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00425071

    authors: Berry A,Jensen RA,Hendry AT

    更新日期:1987-01-01 00:00:00

  • Identification of N-acyl homoserine lactones produced by Gluconacetobacter diazotrophicus PAL5 cultured in complex and synthetic media.

    abstract::The endophytic diazotrophic Gluconacetobacter diazotrophicus PAL5 was originally isolated from sugarcane (Saccharum officinarum). The biological nitrogen fixation, phytohormones secretion, solubilization of mineral nutrients and phytopathogen antagonism allow its classification as a plant growth-promoting bacterium. T...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-012-0794-1

    authors: Nieto-Peñalver CG,Bertini EV,de Figueroa LI

    更新日期:2012-07-01 00:00:00

  • [Cadaverine is an intermediate in the biosynthesis of arthrobactin and ferrioxamine E (author's transl)].

    abstract::Cadaverin was more readily incorporated than lysine into arthrobactin from Arthrobacter pascens and into ferrioxamin E from Streptomyces glaucescens. From a racemic mixture only the L-isomer of lysine is incorporated. The L-lysine decarboxylase activity was measured in vivo and in vitro. The enzyme from Arthrobacter p...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00402309

    authors: Schafft M,Diekmann H

    更新日期:1978-05-30 00:00:00

  • Sinorhizobium fredii HH103 does not strictly require KPS and/or EPS to nodulate Glycyrrhiza uralensis, an indeterminate nodule-forming legume.

    abstract::The Sinorhizobium fredii HH103 rkp-1 region, which is involved in capsular polysaccharide (KPS) biosynthesis, is constituted by the rkpU, rkpAGHIJ, and kpsF3 genes. Two mutants in this region affecting the rkpA (SVQ536) and rkpI (SVQ538) genes were constructed. Polyacrylamide gel electrophoresi...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-011-0729-2

    authors: Margaret-Oliver I,Lei W,Parada M,Rodríguez-Carvajal MA,Crespo-Rivas JC,Hidalgo Á,Gil-Serrano A,Moreno J,Rodríguez-Navarro DN,Buendía-Clavería A,Ollero J,Ruiz-Sainz JE,Vinardell JM

    更新日期:2012-02-01 00:00:00

  • Repression of toxin production by tryptophan in Clostridium botulinum type E.

    abstract::Of the seven amino acids required by Clostridium botulinum type E, tryptophan is the most essential and may provide the cell with nitrogen. The addition of excess tryptophan (10-20 mM) or other nitrogenous nutrients to minimal growth medium markedly decreased toxin formation but did not affect growth in C. botulinum t...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00245225

    authors: Leyer GJ,Johnson EA

    更新日期:1990-01-01 00:00:00

  • Terraforming: synthetic biology's final frontier.

    abstract::Synthetic biology, the design and synthesis of synthetic biological systems from DNA to whole cells, has provided us with the ultimate tools for space exploration and colonisation. Herein, we explore some of the most significant advances and future prospects in the field of synthetic biology, in the context of astrobi...

    journal_title:Archives of microbiology

    pub_type: 信件

    doi:10.1007/s00203-019-01651-x

    authors: Sleator RD,Smith N

    更新日期:2019-08-01 00:00:00

  • Evidence for an arene oxide-NIH shift pathway in the transformation of naphthalene to 1-naphthol by Bacillus cereus.

    abstract::Bacillus cereus ATCC 14579 transformed naphthalene predominately to 1-naphthol. Experiments with [14C]naphthalene showed that over a 24 h period, B. cereus oxidized 5.2% of the added naphthalene. 1-Naphthol accounted for approximately 80% of the total metabolites. B. cereus incubated with naphthalene under the presenc...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00410891

    authors: Cerniglia CE,Freeman JP,Evans FE

    更新日期:1984-08-01 00:00:00

  • Cloning and characterization of a gene encoding the gamma-butyrolactone autoregulator receptor from Streptomyces clavuligerus.

    abstract::With primers designed for the conserved region of the gamma-butyrolactone autoregulator receptor proteins from Streptomyces species, PCR using the Streptomyces clavuligerus genome DNA as a template gave a clear band of 100 bp, the sequence of which revealed high similarity to the expected region of a receptor gene. By...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-004-0697-x

    authors: Kim HS,Lee YJ,Lee CK,Choi SU,Yeo SH,Hwang YI,Yu TS,Kinoshita H,Nihira T

    更新日期:2004-09-01 00:00:00

  • Autoinduction of bacterial luciferase. Occurrence, mechanism and significance.

    abstract::The synthesis of the luminous system of the marine luminous bacterium Photobacterium fischeri is subject to a complex, self-regulated control system called autoinduction. The bacteria produce an autoinducer which accumulates in the medium at a constant rate (as a function of cell growth). When autoinducer reaches a cr...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00446657

    authors: Nealson KH

    更新日期:1977-02-04 00:00:00

  • Ammonia assimilation in the fission yeast Schizosaccharomyces pombe 972.

    abstract::Glutamine synthetase (GS) activity of Schizosaccharomyces pombe 972 was high in ammonia-limited cultures, low in phosphate- and sulphate-limited cultures and not detected in glucose-limited cultures. When ammonia was 'pulsed' into an ammonia-limited culture then GS activity decreased at a rate faster than that calcula...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00549365

    authors: van Andel JG,Brown CM

    更新日期:1977-01-11 00:00:00

  • Molecular characterization of Pseudomonas sp. LDC-5 involved in accumulation of poly 3-hydroxybutyrate and medium-chain-length poly 3-hydroxyalkanoates.

    abstract::Polyhydroxyalkanoates (PHAs) are biological polyesters, of which, Short-Chain-Length-Medium-Chain-Length (SCL-MCL) PHA copolymers are important because of their wide range of applications. The present study focused on molecular characterization of Pseudomonas sp. LDC-5 that is identified as SCL-MCL producer. Phase con...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-007-0265-2

    authors: Sujatha K,Mahalakshmi A,Shenbagarathai R

    更新日期:2007-11-01 00:00:00

  • The occurrence of teichoic acids in streptomycetes.

    abstract::The presence of teichoic acids in a number of streptomycetes led to the conclusion that these biopolymers were widely spread in genus Streptomyces. The nature of the teichoic acid present in the mycelium was determined by extracting it with 10% trichloroacetic acid, precipitating it with ethanol and identifying the pr...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00421893

    authors: Naumova IB,Kuznetsov VD,Kudrina KS,Bezzubenkova AP

    更新日期:1980-05-01 00:00:00

  • Ethanol-sensitive mutants of Saccharomyces cerevisiae.

    abstract::Saccharomyces cerevisiae mutants unable to grow at ethanol concentrations at which the wild type strain S288C does grow, have been isolated. Some of them show additional phenotypic alterations in colony size, temperature sensitivity and viability in ethanol, which cosegregate with the growth sensitivity in ethanol. 21...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00412799

    authors: Aguilera A,Benítez T

    更新日期:1986-01-01 00:00:00

  • Molecular characterization and isolation of cytochrome P450 genes from the filamentous fungus Aspergillus oryzae.

    abstract::We explored the molecular diversity of cytochrome P450 genes in the filamentous fungus Aspergillus oryzae using bioinformatic and experimental approaches. Based on bioinformatic annotation, we found 155 putative genes of cytochromes P450 in the whole genome sequence; however, 13 of 155 appeared to be pseudogenes due t...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-010-0562-z

    authors: Nazmul Hussain Nazir KH,Ichinose H,Wariishi H

    更新日期:2010-05-01 00:00:00

  • An examination of the diversity of a novel Campylobacter reservoir.

    abstract::The diversity of eukaryotic populations, in particular protozoa, in the water supplies of intensively reared broilers has not been previously studied. This important food-rearing environment was screened for the molecular diversity of eukaryotes by the analysis of PCR-amplified 18S rRNA. DNA was extracted from filtere...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-006-0119-3

    authors: Snelling WJ,McKenna JP,Hack CJ,Moore JE,Dooley JS

    更新日期:2006-07-01 00:00:00

  • Halomarina rubra sp. nov., isolated from a marine solar saltern.

    abstract::Halophilic archaeal strain ZS-47-ST was isolated from Zhoushan marine solar saltern, China. Cells were pleomorphic, stained Gram-negative, and formed red-pigmented colonies on agar plate. Strain ZS-47-ST was able to grow at 20-50 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 3.4 M), at 0.005-1.0 M MgCl2 (optimum 0.03...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-017-1420-z

    authors: Zhou Y,Li Y,Lü ZZ,Cui HL

    更新日期:2017-12-01 00:00:00

  • Purification and characterization of benzyl alcohol- and benzaldehyde- dehydrogenase from Pseudomonas putida CSV86.

    abstract::Pseudomonas putida CSV86 utilizes benzyl alcohol via catechol and methylnaphthalenes through detoxification pathway via hydroxymethylnaphthalenes and naphthaldehydes. Based on metabolic studies, benzyl alcohol dehydrogenase (BADH) and benzaldehyde dehydrogenase (BZDH) were hypothesized to be involved in the detoxifica...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-011-0697-6

    authors: Shrivastava R,Basu A,Phale PS

    更新日期:2011-08-01 00:00:00

  • Sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species.

    abstract::Recent studies indicated that bioluminescence of the marine bacterium Vibrio harveyi may both stimulate DNA repair and contribute to detoxification of deleterious oxygen derivatives. Therefore, it was also proposed that these reactions can be considered biological roles of bacterial luminescence and might act as evolu...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-005-0764-y

    authors: Lyzeń R,Wegrzyn G

    更新日期:2005-03-01 00:00:00

  • Separation of algal mixtures and bacterial mixtures with flow-microfluorometer using chlorophyll and ethidium bromide fluorescence.

    abstract::The applicability of flow-microfluorometer to separate microbial cells was demonstrated with algal and bacterial cells. Algal mixtures were sorted according to the natural chlorophyll fluorescence and the bacterial mixtures were sorted according to the fluorescence of ethidium bromide-stained nucleic acid. ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00423075

    authors: Paau AS,Cowles JR,Oro J,Bartel A,Hungerford E

    更新日期:1979-03-12 00:00:00

  • The bacterial oxidation of nicotinic acid. N-formylmaleamic and N-formylfumaramic acids.

    abstract::N-Formylmaleamic acid, a probable intermediate in the bacterial metabolism of nicotinic acid, has been synthesized by photoisomerization of its trans isomer, N-formylfumaramic acid. The compound previously reported to be N-formylmaleamic acid has been shown to be N-formylfumaramic acid. ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00416972

    authors: Behrman EJ

    更新日期:1976-10-11 00:00:00

  • Expression of bvgAS of Bordetella pertussis represses flagellar biosynthesis of Escherichia coli.

    abstract::BvgAS is a two-component system of Bordetella pertussis involved in the reciprocal regulation of the virulence genes and the flagellar biosynthesis. In this study, we found that expression of bvgAS in Escherichia coli also results in reduced motility. The repression was relieved by the addition of known chemical modul...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030050688

    authors: Han YW,Uhl MA,Han SJ,Shi W

    更新日期:1999-01-01 00:00:00