Expression of bvgAS of Bordetella pertussis represses flagellar biosynthesis of Escherichia coli.

Abstract:

:BvgAS is a two-component system of Bordetella pertussis involved in the reciprocal regulation of the virulence genes and the flagellar biosynthesis. In this study, we found that expression of bvgAS in Escherichia coli also results in reduced motility. The repression was relieved by the addition of known chemical modulators of BvgAS such as MgSO4 and nicotinic acid, indicating that functional BvgAS proteins are required for the negative control of E. coli motility. In addition, BvgAS repressed the transcription of the flhDC master operon of E. coli, which consequently caused non-flagellation on the cell surface. However, expression of BvgAS had no effect on stress-resistant motile mutants of E. coli. These data suggest that E. coli may have BvgA-like protein(s) involved in the regulatory interactions between the stress response and the flagellar biosynthesis.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Han YW,Uhl MA,Han SJ,Shi W

doi

10.1007/s002030050688

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

127-30

issue

2

eissn

0302-8933

issn

1432-072X

journal_volume

171

pub_type

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