Abstract:
:Pulicat Lake sediments are often severely polluted with the toxic heavy metal mercury. Several mercury-resistant strains of Bacillus species were isolated from the sediments and all the isolates exhibited broad spectrum resistance (resistance to both organic and inorganic mercuric compounds). Plasmid curing assay showed that all the isolated Bacillus strains carry chromosomally borne mercury resistance. Polymerase chain reaction and southern hybridization analyses using merA and merB3 gene primers/probes showed that five of the isolated Bacillus strains carry sequences similar to known merA and merB3 genes. Results of multiple sequence alignment revealed 99% similarity with merA and merB3 of TnMERI1 (class II transposons). Other mercury resistant Bacillus species lacking homology to these genes were not able to volatilize mercuric chloride, indicating the presence of other modes of resistance to mercuric compounds.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Kannan SK,Mahadevan S,Krishnamoorthy Rdoi
10.1007/s00203-006-0088-6keywords:
subject
Has Abstractpub_date
2006-04-01 00:00:00pages
202-11issue
3eissn
0302-8933issn
1432-072Xjournal_volume
185pub_type
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