Abstract：
:Edwardsiella tarda (E. tarda) is distributed widely in a variety of hosts including humans, other mammals and fish, and it is worthwhile to notice that E. tarda -caused fish infections lead to the most important bacterial disease in fish. Considering Eha acting as a transcriptional regulator in E. tarda strain ET13 have been reported previously, to better understand its pathogenesis due to this, a type of cell of epithelial cell line (Caco-2) infection model for the pathogen was established in the laboratory. We focused on studying various parameters such as lactate dehydrogenase release (to measure cytotoxicity) and cell adhesions, both of which are related to the bacterial pathogenesis. Furthermore biofilm formation, hemolytic activity, and adhesion to Caco-2 cells were decreased in an E.tarda mutant strain with deletion in-frame isogenic gene eha (∆eha) compared to the wild-type and the complementary strain eha+ (an engineered construct of ∆eha expressing eha); Meanwhile, we found that hemolytic activity and biofilm formation were significantly enhanced in the strain eha+. Moreover, the ∆eha strain had attenuated pathogenicity in the zebrafish infection model. The data also demonstrated that the series of genes fimA, esrB, gadB, mukF, katB, and katG are regulated by eha based on a quantitative reverse transcription polymerase chain reaction tests and analysis. Thus our research data indicated that eha has an impact on hemolytic activity, biofilm formation, adhesion, and pathogenicity of pathogenic strain ET13 and plays an essential role in manifesting the virulence factors.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Hassan HA,Ding X,Zhang X,Zhu Gdoi
10.1007/s00203-019-01794-xsubject
Has Abstractpub_date
2020-05-01 00:00:00pages
835-842issue
4eissn
0302-8933issn
1432-072Xpii
10.1007/s00203-019-01794-xjournal_volume
202pub_type
杂志文章abstract：:In autotrophic methanogens, pyruvate oxidoreductase (POR) plays a key role in the assimilation of CO(2) and the biosynthesis of organic carbon. This enzyme has been purified to homogeneity, and the genes from Methanococcus maripaludis were sequenced. The purified POR contained five polypeptides with molecular masses o...
journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：2003-06-01 00:00:00
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journal_title：Archives of microbiology
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pub_type： 杂志文章
doi：10.1007/BF00248837
更新日期：1990-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0431-1
更新日期：2009-02-01 00:00:00
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更新日期：2020-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00203-016-1230-8
更新日期：2016-10-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0119-3
更新日期：2006-07-01 00:00:00
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journal_title：Archives of microbiology
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doi：10.1007/s00203-003-0580-1
更新日期：2003-09-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100318
更新日期：2001-10-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00738543
更新日期：1978-06-26 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00288712
更新日期：1993-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0593-9
更新日期：2003-10-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s002030100263
更新日期：2001-04-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00403209
更新日期：1980-03-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446663
更新日期：1977-02-04 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1304-7
更新日期：2017-03-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：2020-07-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：2006-07-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
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更新日期：1998-03-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1988-01-01 00:00:00
abstract：:Acyl carrier protein (ACP) is a small acidic protein that acts as an essential cofactor in many biosynthetic pathways depending on acyl transfer reactions. In this work, a Vibrio anguillarum ACP encoding gene, acpV, was first cloned from the chromosome of a virulent V. anguillarum strain MVM425. acpV was over-expresse...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0058-4
更新日期：2006-03-01 00:00:00
abstract：:Several studies have investigated the effects of ionizing and non-ionizing radiations on microorganisms. However, the interaction between the magnetic field radiations and bacteria is less studied. The aim of our study was to study the effect of static magnetic field on the biofilm formation in Pseudomonas aeruginosa ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01719-8
更新日期：2020-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-001-0378-y
更新日期：2002-02-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1987-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00249083
更新日期：1990-01-01 00:00:00
abstract：:A thermostable homodimeric isocitrate dehydrogenase from the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus was purified and characterized. The mol. mass of the isocitrate dehydrogenase subunit was 42 kDa as determined by SDS-PAGE. Following separation by SDS-PAGE, A. fulgidus isocitrate dehydrogen...
journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1997-11-01 00:00:00
