Halomarina rubra sp. nov., isolated from a marine solar saltern.

Abstract:

:Halophilic archaeal strain ZS-47-ST was isolated from Zhoushan marine solar saltern, China. Cells were pleomorphic, stained Gram-negative, and formed red-pigmented colonies on agar plate. Strain ZS-47-ST was able to grow at 20-50 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 3.4 M), at 0.005-1.0 M MgCl2 (optimum 0.03 M), and at pH 5.5-9.5 (optimum pH 6.5-7.5). The cells lysed in distilled water and the minimal NaCl concentration to prevent cell-lysis was 5% (w/v). The major polar lipids were C20C20 and C20C25 diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, glucosyl mannosyl glucosyl diether, and three unidentified glycolipids. The 16S rRNA gene and rpoB' gene of strain ZS-47-ST were phylogenetically related to the corresponding genes of Halomarina oriensis JCM 16495T (98.57 and 92.94% similarities, respectively) and Halomarina salina CGMCC 1.12543T (97.96 and 93.65% similarities, respectively). The DNA G + C content of strain ZS-47-ST was 64.6 mol % (T m). The phenotypic, chemotaxonomic, and phylogenetic properties suggested that strain ZS-47-ST (=CGMCC 1.12563T = JCM 30037T) represents a new species of Halomarina, for which the name Halomarina rubra sp. nov. is proposed.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Zhou Y,Li Y,Lü ZZ,Cui HL

doi

10.1007/s00203-017-1420-z

subject

Has Abstract

pub_date

2017-12-01 00:00:00

pages

1431-1435

issue

10

eissn

0302-8933

issn

1432-072X

pii

10.1007/s00203-017-1420-z

journal_volume

199

pub_type

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