Abstract：
:Serine (Ser)/threonine (Thr) or tyrosine (Tyr) protein kinases in eukaryotes contain RDxKxxN or RDx(A/R)A(A/R)N sequences, respectively, in the catalytic loop. Myxococcus xanthus DspB is a dual-specificity kinase that contains an atypical sequence, RDVAQKN, in the catalytic loop. The DspB mutant (A165K), which contains the canonical RDxKxxN motif, had an approximate 1.3-fold increase in kinase activity toward myelin basic protein (MBP). Arginine-aspartate (RD) kinases carry a conserved Arg immediately preceding the catalytic Asp that is required for autophosphorylation of the activation loop. DspB belongs to the RD kinase family and contains one Ser residue (Ser-190) and one Thr residue (Thr-194) in the activation loop. Mutation of Ser-190 or Thr-194 to Ala did not significantly affect the kinase activity toward MBP. We previously reported that four M. xanthus eukaryotic-like kinases (EPKs) are autophosphorylated on Tyr residues. These EPKs contain six Tyr residues at homologous positions, and five of those Tyr residues, Y25, Y102, Y145, Y173, and Y205, are conserved in DspB. DspB is mainly autophosphorylated on Y145, and a Y145F mutant has reduced kinase activity, suggesting that autophosphorylation of the Tyr residue of DspB may be required for high-level kinase activity.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Kimura Y,Urata Mdoi
10.1007/s00203-015-1181-5subject
Has Abstractpub_date
2016-04-01 00:00:00pages
219-26issue
3eissn
0302-8933issn
1432-072Xpii
10.1007/s00203-015-1181-5journal_volume
198pub_type
杂志文章abstract：:The synthesis of beta-glucanase either by cells or by protoplasts of the yeast Pichia polymorpha has been found to occur in the presence of 2-deoxy-D-glucose in the growth medium. On the other hand, the synthesis of typical extracellular proteins such as invertase and acid phosphatase is strongly affected by the prese...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447154
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429646
更新日期：1987-06-01 00:00:00
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journal_title：Archives of microbiology
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doi：10.1007/s00203-007-0339-1
更新日期：2008-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2001-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF01692864
更新日期：1996-03-01 00:00:00
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更新日期：1981-04-01 00:00:00
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更新日期：2001-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00421893
更新日期：1980-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00413014
更新日期：1984-06-01 00:00:00
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更新日期：2020-03-01 00:00:00
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更新日期：2012-02-01 00:00:00
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更新日期：1984-08-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446777
更新日期：1986-07-01 00:00:00
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doi：10.1007/s00203-004-0666-4
更新日期：2004-05-01 00:00:00
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更新日期：2019-08-01 00:00:00
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更新日期：1983-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00407938
更新日期：1983-02-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050624
更新日期：1998-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-09-29 00:00:00
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更新日期：2003-04-01 00:00:00
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更新日期：1989-01-01 00:00:00
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更新日期：2006-07-01 00:00:00
