Abstract：
:The evolutionarily conserved serine/threonine kinase TOR recruits different subunits to assemble the Target of Rapamycin Complex 1 (TORC1), which is inhibited by rapamycin and regulates ribosome biogenesis, autophagy, and lipid metabolism by regulating the expression of lipogenic genes. In addition, TORC1 participates in the cell cycle, increasing the length of the G2 phase. In the present work, we investigated the effect of rapamycin on cell growth, cell morphology and neutral lipid metabolism in the phytopathogenic fungus Ustilago maydis. Inhibition of TORC1 by rapamycin induced the formation of septa that separate the nuclei that were formed after mitosis. Regarding neutral lipid metabolism, a higher accumulation of triacylglycerols was not detected, but the cells did contain large lipid bodies, which suggests that small lipid bodies became fused into big lipid droplets. Vacuoles showed a similar behavior as the lipid bodies, and double labeling with Blue-CMAC and BODIPY indicates that vacuoles and lipid bodies were independent organelles. The results suggest that TORC1 has a role in cell morphology, lipid metabolism, and vacuolar physiology in U. maydis.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Romero-Aguilar L,Guerra-Sánchez G,Tenorio EP,Tapia-Rodriguez M,Matus-Ortega G,Flores-Herrera O,González J,Pardo JPdoi
10.1007/s00203-020-01833-ysubject
Has Abstractpub_date
2020-07-01 00:00:00pages
1211-1221issue
5eissn
0302-8933issn
1432-072Xpii
10.1007/s00203-020-01833-yjournal_volume
202pub_type
杂志文章abstract：:The starvation survivability of seven Thermococcus strains isolated from four Japanese oil reservoirs was compared with that of Thermococcus strains from marine hydrothermal fields. 16S rDNA analyses showed the isolates to be closely related to Thermococcus litoralis. Growth of the isolates was dependent on amino acid...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100318
更新日期：2001-10-01 00:00:00
abstract：:Salt-sensitive mutants of Synechocystis were obtained by random cartridge mutagenesis, and one mutant (mutant 4) was characterized in detail. The salt tolerance of mutant 4 was reduced to about 20% of that of the wild-type. This was caused by a defect in the biosynthetic pathway of the osmoprotective compound glucosyl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050360
更新日期：1996-08-01 00:00:00
abstract：:The distribution of sex pheromone induced aggregation substance was studied on the cell surface of various Enterococcus faecalis strains. In the accompanying paper we have shown that the aggregation substance appears as a layer of hairlike structures. Using direct and indirect immunogold technique, transmission electr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454864
更新日期：1989-01-01 00:00:00
abstract：:Inorganic polyphosphate (iPoP)-linear chains of up to hundreds of phosphate residues-is ubiquitous in nature and appears to be involved in many different cellular processes. In Saccharomyces cerevisiae, iPoP has been detected in high concentrations, especially after transfer of phosphate-deprived cells to a high-phosp...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0031-2
更新日期：2005-11-01 00:00:00
abstract：:The ADPglucose pyrophosphorylases from Rhodopseudomonas sphaeroides and Rhodopseudomonas gelatinosa are activated by fructose-6-phosphate, pyruvate and fructose-1,6 biophosphate-P2. The effects of the activators are to increase significantly the Vmax of ADPglucose synthesis and to lower the S0.5 values (concentration ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00421887
更新日期：1980-05-01 00:00:00
abstract：:Serine/threonine protein kinases (STPK) play a major role in the physiology and pathogenesis of Mycobacterium tuberculosis. Here, we have examined the role of pknE, a STPK in the adaptive responses of M. tuberculosis using a deletion mutant ΔpknE. The survival of ΔpknE was assessed in the presence of stress (pH, surfa...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0848-4
更新日期：2013-01-01 00:00:00
abstract：:Video-enhanced light microscopy of the apical and subapical regions of growing hyphae of several fungal species revealed the existence of momentary synchronized motions of subcellular organelles. First discovered in a temperature-sensitive morphological mutant (ramosa-1) of Aspergillus niger, these seemingly spontaneo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0771-z
更新日期：2005-05-01 00:00:00
abstract：:The family Chlamydiaceae contains nine species pathogenic to humans and animals, but their routine identification is hampered by inadequate detection methods. In an attempt to find a new region for PCR detection and discrimination of the Chlamydiaceae species, the 3' end of the omp2 gene of Chlamydiaceae has been exam...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0757-2
更新日期：2005-03-01 00:00:00
abstract：:During cultivation of a gastric fungus, Coniochaeta polymorpha, growth of Nocardia colonies on top of the fungal culture raised the question whether bacteria originated from inside of fungus. In this study, the likelihood of intracellular origin of bacteria as well as interaction of two microorganisms was assessed. Fl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02122-4
更新日期：2021-01-02 00:00:00
abstract：:Edwardsiella tarda (E. tarda) is distributed widely in a variety of hosts including humans, other mammals and fish, and it is worthwhile to notice that E. tarda -caused fish infections lead to the most important bacterial disease in fish. Considering Eha acting as a transcriptional regulator in E. tarda strain ET13 ha...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01794-x
更新日期：2020-05-01 00:00:00
abstract：:Coenzyme F420 is a 8-hydroxy-5-deazaflavin present in methanogenic bacteria. We have investigated whether the pyrimidine ring of the deazaflavin originates from guanine as in flavin biosynthesis, in which the pyrimidine ring of guanine is conserved. For this purpose the incorporation of [2-14C]guanine and of [8-14C]gu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00410735
更新日期：1984-04-01 00:00:00
abstract：:The Roseobacter clade, belonging to the family Rhodobacteraceae of the class Alphaproteobacteria, is one of the major bacterial groups in marine environments. A remarkable wealth of diverse large plasmids has been detected in members of this lineage. Here, we analysed the genome structure and extrachromosomal DNA cont...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0535-2
更新日期：2010-02-01 00:00:00
abstract：:Monoclonal antibodies have been used to determine the presence of cellobiohydrolases I and II (CBH I and II), and endoglucanase I (EG I) on the surface of conidia from Trichoderma reesei QM 9414 and RUT C-30, and 8 other Trichoderma species. For this purpose, proteins were released from the conidial surface by treatme...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245356
更新日期：1991-01-01 00:00:00
abstract：:Clostridium aminobutyricum ferments 4-aminobutyrate via succinic semialdehyde, 4-hydroxybutyrate, 4-hydroxybutyryl-CoA and crotonyl-CoA to acetate and butyrate. The genes coding for the enzymes that catalyse the interconversion of these intermediates are arranged in the order abfD (4-hydroxybutyryl-CoA dehydratase), a...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030000195
更新日期：2000-09-01 00:00:00
abstract：:It has been shown previously that externally added glycine betaine is accumulated in Escherichia coli in response to the external osmotic strength. Here we have shown, by using nuclear magnetic resonance spectroscopy and radiochemical methods, that E. coli growing in a glucose-mineral medium of elevated osmotic streng...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00492896
更新日期：1987-02-01 00:00:00
abstract：:The nitrite oxidoreductase (NOR) from the facultative nitrite-oxidizing bacterium Nitrobacter hamburgensis X14 was investigated genetically. In order to develop a probe for the gene norB, the N-terminal amino acid sequence of the NOR beta-subunit (NorB) was determined. Based on that amino acid sequence, an oligonucleo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245305
更新日期：1993-01-01 00:00:00
abstract：:Fermentative degradation of phenol was studied using a non-methanogenic, pasteurised enrichment culture containing two morphologically different bacteria. Phenol was fermented to benzoate, acetate and butyrate and their relative occurrence depended on the concentration of hydrogen. Proportionately more benzoate was fo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030000160
更新日期：2000-05-01 00:00:00
abstract：:Catalytic properties and membrane associations of the phosphatidylglycerophosphate (PGP) and phosphatidylserine (PS) synthases of Rhodobacter sphaeroides were examined to further characterize sites of phospholipid biosynthesis. In preparations of cytoplasmic membrane (CM) enriched in these activities, apparent Km valu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00456090
更新日期：1989-01-01 00:00:00
abstract：:Calcium was found to stimulate stalk development in Caulobacter crescentus and to relieve the inhibition of development long known to be caused by phosphate. This suggested that phosphate inhibition could be attributed to its interaction with Ca2+, thereby depriving the cells of a factor that promoted development. Cal...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413014
更新日期：1984-06-01 00:00:00
abstract：:In the Tat protein export pathway of Gram-negative bacteria, TatA and TatB are homologous proteins that carry out distinct and essential functions in separate sub-complexes. In contrast, Gram-positive Tat systems usually lack TatB and the TatA protein is bifunctional. We have used a mutagenesis approach to delineate T...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0699-4
更新日期：2011-08-01 00:00:00
abstract：:Calcium is essential for the growth of rhizobia and the formation of nitrogen-fixing root-nodules on legumes, but its precise role in these processes remains unknown. We have found that Rhizobium fredii USDA208 accumulates a major 38 kDa protein when grown in media supplemented with 0.3-2 microM Cacl2. We have purifie...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00248480
更新日期：1993-01-01 00:00:00
abstract：:Lipase is an important commercial enzyme with unique and versatile biotechnological applications. This study was conducted to biosynthesize and characterizes alkaliphilic lipase by Exiguobacterium sp. strain AMBL-20T isolated from the glacial water samples of the northeastern (Gilgit-Baltistan) region of Pakistan. The...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02133-1
更新日期：2020-12-15 00:00:00
abstract：:Of 147 microorganisms isolated from a loamy sand, 71 showed good growth with lindane (gamma-1,2,3,4,5,6-hexachlorocyclohexane) and produced chloride in an aqueous medium. Thirteen soil microorganisms were selected to study the utilization of lindane. Lindane was metabolized by the microbes to gamma-2,3,4,5,6-pentachlo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454850
更新日期：1976-07-01 00:00:00
abstract：:L-(+)-lactate dehydrogenase (LDH) from Staphylococcus epidermidis ATCC 14990 was purified by affinity chromatography. The purified enzyme was specifically activated by fructose-1,6-diphosphate (FDP). The concentration of FDP required for 50% maximal activity was about 0.15 mM. The enzyme activity was inhibited by aden...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447150
更新日期：1975-11-07 00:00:00
abstract：:Expression of unnecessary proteins is known to reduce the growth rate of Escherichia coli. This reduction in growth rate, due to the diversion of cellular resources from making essential proteins, is called cost. Cellular resources depend upon the macromolecular content of the cell. Cost phenomenon lies in the process...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-0967-1
更新日期：2014-04-01 00:00:00
abstract：:To overcome the extracellular salt stress, Methanohalophilus portucalensis FDF1(T) synthesizes the compatible solute betaine through the methylation of glycine, sarcosine, and N,N-dimethylglycine. S-adenosylmethionine (AdoMet) is the methyl donor. The enzyme sarcosine dimethylglycine N-methyltransferase (SDMT) of M. p...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0501-z
更新日期：2009-10-01 00:00:00
abstract：:The thermophilic bacterium Thermoanaerobacter tengcongensis has two single-stranded DNA-binding (SSB) proteins, designated TteSSB2 and TteSSB3. In a SSB complementation assay in Escherichia coli, only TteSSB3 took over the in vivo function of EcoSSB. We have cloned the ssb genes obtained by PCR and have developed E. c...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0366-6
更新日期：2008-07-01 00:00:00
abstract：:We describe a convenient method for the in vivo construction of large plasmids that possess a multitude of restriction sites. A large (23 kbases) circular self-replicating plasmid carrying a partial LEU2-d gene was cotransformed with a circular non-replicating plasmid carrying the entire LEU2 gene. In vivo recombinati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00409661
更新日期：1989-01-01 00:00:00
abstract：:A novel gram-negative, aerobic, non-spore-forming, rod-shaped and non-nitrogen fixing bacterium named strain 24NRT was isolated from wild Lilium pumilum bulbs in Fuping, Baoding City, Hebei province, PR China. The 16S rRNA gene sequences of strains 24NRT showed the highest similarity to Neorhizobium alkalisoli DSM 218...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01774-1
更新日期：2020-04-01 00:00:00
abstract：:The upstream region of the isocitrate lyase gene (UPR-ICL, 1530bp) of an n-alkane-utilizable yeast, Candida tropicalis, induced gene expression in another yeast, Saccharomyces cerevisiae, when the yeasts were grown on acetate. Surprisingly, UPR-ICL displayed the same regulatory function in the bacterium Escherichia co...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00404204
更新日期：1995-05-01 00:00:00
