Abstract：
:A novel coccoid-shaped, hyperthermophilic, heterotrophic member of the archaea was isolated from a shallow marine hydrothermal system at Vulcano Island, Italy. The isolate grew between 56 and 90 degrees C with an optimum around 85 degrees C. The pH range for growth was 6.5 to 10.5, with an optimum around 9.0. Polysulfide and elemental sulfur were reduced to H2S. Sulfur stimulated the growth rate. The isolate fermented yeast extract, peptone, meat extract, tryptone, and casein. Isovalerate, isobutyrate, propionate, acetate, CO2, NH3, and H2S (in the presence of S degrees ) were detected as end products. Growth was not inhibited by H2. Based on DNA-DNA hybridization and 16S rRNA partial sequences, the new isolate represents a new species of Thermococcus, which we named Thermococcus alcaliphilus. The type strain is isolate AEDII12 (DSM 10322).
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Keller M,Braun FJ,Dirmeier R,Hafenbradl D,Burggraf S,Rachel R,Stetter KOsubject
Has Abstractpub_date
1995-12-01 00:00:00pages
390-5issue
6eissn
0302-8933issn
1432-072Xjournal_volume
164pub_type
杂志文章abstract：:The natural role of antibiotics in the ecology of Streptomyces is debated and still largely unknown. The predatory myxobacteria and many other genera of prokaryotic epibiotic and wolfpack predators across different taxa possess secondary metabolites with antimicrobial action, and these compounds have a role in predati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-0961-7
更新日期：2014-04-01 00:00:00
abstract：:A highly efficient method of transposon mutagenesis was developed for genetic analysis of Xanthobacter autotrophicus Py2. The method makes use of a transposon delivery vector that encodes a hyperactive Tn 5 transposase that is 1,000-fold more active than the wild-type transposase. In this construct, the transposase is...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0442-2
更新日期：2002-09-01 00:00:00
abstract：:The maleylacetate reductase of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 has been purified 50-fold. The enzyme converted 2-chloromaleylacetate to 3-oxoadipate with temporary occurrence of maleylacetate; 1 mol of chloride was eliminated during the conversion of 1 mol of 2-chloro- and 2,3-dichloromaleyl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00276301
更新日期：1992-01-01 00:00:00
abstract：:A bacterium capable of using the carbamate insecticide carbofuran as a sole source of carbon and energy, was isolated from soil. The ability to catabolise carbofuran phenol, produced by cleavage of the carbamate ester linkage of the insecticide, was lost at very high frequency when the bacterium was grown in the absen...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245249
更新日期：1992-01-01 00:00:00
abstract：:Serine/threonine protein kinases (STPK) play a major role in the physiology and pathogenesis of Mycobacterium tuberculosis. Here, we have examined the role of pknE, a STPK in the adaptive responses of M. tuberculosis using a deletion mutant ΔpknE. The survival of ΔpknE was assessed in the presence of stress (pH, surfa...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0848-4
更新日期：2013-01-01 00:00:00
abstract：:Chlorella fusca, strain 211-15, cells degreened in a nitrogen-deficient mineral growth medium in the light for 4-6 weeks were regreened for up to 24 hrs in a nitrogen rich medium that leads to synchronous cell division at 24-26 hrs. Structural changes in the plastid membranes during the regreening period were observed...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00436359
更新日期：1975-05-05 00:00:00
abstract：:Inorganic polyphosphate (iPoP)-linear chains of up to hundreds of phosphate residues-is ubiquitous in nature and appears to be involved in many different cellular processes. In Saccharomyces cerevisiae, iPoP has been detected in high concentrations, especially after transfer of phosphate-deprived cells to a high-phosp...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0031-2
更新日期：2005-11-01 00:00:00
abstract：:DNA sequence analysis of the structural urease genes from Staphylococcus xylosus revealed that three enzyme subunits are encoded in the order of 11,000, 15,400 and 61,000 (mol. mass), which correspond to the single polypeptide chain of jack bean urease (90,800). Comparing the deduced amino acid sequence of S. xylosus ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00288947
更新日期：1994-01-01 00:00:00
abstract：:The radiation resistant bacteria Micrococcus radiophilus and M. radioproteolyticus were studied by thin sectioning and freez-etching techniques and the two species were found to be similar in the fine structure. The only significant difference was in the appearance of the surfaces of the cell walls in freeze-etched pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425346
更新日期：1976-04-01 00:00:00
abstract：:We report the phylogenetic and physiological characterization of a mesophilic and halophilic member of the filamentous anoxygenic phototrophic (FAP) bacteria, provisionally named ' Candidatus Chorothrix halophila' gen. nov. sp. nov., that has been maintained in a highly enriched culture in our laboratory for over a de...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0615-7
更新日期：2004-01-01 00:00:00
abstract：:Bacteriochlorophyll (BChl) c in whole cells of Chlorobium tepidum grown at 46 degrees C changed into bacteriopheophytin (BPhe) c within 10 days after reaching full growth. When a small amount of C. tepidum cells in which BChl c had been completely pheophytinized were transferred to a new culture medium, normal growth ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050737
更新日期：1999-07-01 00:00:00
abstract：:Calcium is essential for the growth of rhizobia and the formation of nitrogen-fixing root-nodules on legumes, but its precise role in these processes remains unknown. We have found that Rhizobium fredii USDA208 accumulates a major 38 kDa protein when grown in media supplemented with 0.3-2 microM Cacl2. We have purifie...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00248480
更新日期：1993-01-01 00:00:00
abstract：:To elucidate the physiological adaptation of Escherichia coli due to cra gene knockout, a total of 3,911 gene expressions were investigated by DNA microarray for continuous culture. About 50 genes were differentially regulated for the cra mutant. TCA cycle and glyoxylate shunt were down-regulated, while pentose phosph...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0406-2
更新日期：2008-11-01 00:00:00
abstract：:The utilization of carnitine and carnitine derivatives (O-acylcarnitines, carnitine carboxylderivatives) and structure-related trimethylammonium-compounds (betaines and nitrogen-bases) by Acinetobacter calcoaceticus was studied by means of the control of growth and the quantitative detection of metabolites. The strain...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429336
更新日期：1977-03-01 00:00:00
abstract：:An alkaliphilic actinobacterial strain, designated Hz 6-5T, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China. The isolate formed light yellow-colored colonies and its cells were Gram-staining positive, non-motile, and non-spore-forming short rods. The strain was aerobic with optimal gro...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02073-w
更新日期：2020-10-26 00:00:00
abstract：:Cellulomonas uda (DSM 20108/ATCC 21399) is one of the few described cellulolytic facultative anaerobes. Based on these characteristics, we initiated a physiological study of C. uda with the aim to exploit it for cellulase production in simple bioreactors with no or sporadic aeration. Growth, cellulase activity and fer...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1230-8
更新日期：2016-10-01 00:00:00
abstract：:The role of the genomic bipyrimidine nucleotide frequency in pyrimidine dimer formation caused by germicidal UV radiation was studied in three microbial reference organisms (Escherichia coli K12, Deinococcus radiodurans R1, spores and cells of Bacillus subtilis 168). The sensitive HPLC tandem mass spectrometry assay w...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0579-3
更新日期：2010-07-01 00:00:00
abstract：:Curcumin is a yellow-orange compound derived from the root of Curcuma longa (Zingiberaceae family), that has been used as a medicine, spice and coloring agent. Curcumin has proved nontoxic in a number of cell culture and whole animal studies. Curcumin has, however, been reported to have bactericidal effects at very hi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00414437
更新日期：1989-01-01 00:00:00
abstract：:Acinetobacter strain IVS-B aerobically grows on isovalerate as sole carbon and energy source. Isovalerate is metabolised via isovaleryl-CoA, an intermediate of the oxidative (S)-leucine degradation pathway. A 3-methylglutaconyl-CoA hydratase (EC 4.2.1.18) was purified 65-fold to apparent homogeneity from cell-free ext...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0095-7
更新日期：2006-05-01 00:00:00
abstract：:The antimicrobial properties of copper ions have been known for a long time. However, the exact mechanism of action of the transition metal on microorganisms has long been unclear. X-ray absorption near-edge structure (XANES) spectroscopy at the Cu K edge allows the determination of copper speciation in Staphylococcus...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1454-2
更新日期：2018-04-01 00:00:00
abstract：:A Gram reaction-negative, strictly aerobic, non-motile, translucent and rod-shaped bacterium (designated W1-2-4(T)) isolated from tap water was characterized by a polyphasic approach to clarify its taxonomic position. Strain W1-2-4(T) was observed to grow optimally at 25-30 °C and at pH 6.5 on nutrient agar. Phylogene...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1237-1
更新日期：2016-10-01 00:00:00
abstract：:The functional pmoA gene is frequently used to probe the diversity and phylogeny of methane-oxidizing bacteria (MOB) in various environments. Here, we compared the similarities between the pmoA gene and the corresponding 16S rRNA gene sequences of 77 described species covering gamma- and alphaproteobacterial methanotr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1222-8
更新日期：2016-09-01 00:00:00
abstract：:The main aim of our study was to determine the physiological function of NagA enzyme in the Listeria monocytogenes cell. The primary structure of the murein of L. monocytogenes is very similar to that of Escherichia coli, the main differences being amidation of diaminopimelic acid and partial de-N-acetylation of gluco...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0752-3
更新日期：2012-04-01 00:00:00
abstract：:The L-alanine mediated germination of food isolated Bacillus cereus DSA 1 spores, which lacked an intact exosporium, increased in the presence of D-cycloserine (DCS), which is an alanine racemase (Alr) inhibitor, reflecting the activity of the Alr enzyme, capable of converting L-alanine to the germination inhibitor D-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0946-y
更新日期：2014-02-01 00:00:00
abstract：:Triacylglycerol granules synthesized and accumulated by Rhodococcus opacus and Rhodococcus ruber were isolated by glycerol density gradient centrifugation. Whereas only one type of granule could be isolated from R. opacus, two types of granules with different specific densities were isolated from R. ruber. Both types ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-001-0355-5
更新日期：2001-12-01 00:00:00
abstract：:During cultivation of a gastric fungus, Coniochaeta polymorpha, growth of Nocardia colonies on top of the fungal culture raised the question whether bacteria originated from inside of fungus. In this study, the likelihood of intracellular origin of bacteria as well as interaction of two microorganisms was assessed. Fl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02122-4
更新日期：2021-01-02 00:00:00
abstract：:Aflatoxins are highly toxic and carcinogenic fungal secondary metabolites. At least 18 enzyme activities are required for aflatoxin biosynthesis in the filamentous fungus Aspergillus parasiticus. One of these enzymes, versicolorin B synthase (VBS), catalyzes bisfuran ring closure in versiconal hemiacetal (a reaction n...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0700-6
更新日期：2004-09-01 00:00:00
abstract：:Autotrophic cultures of the facultative chemolithotroph Alcaligenes eutrophus have been found to excrete glycollate. This excretion was greatly stimulated by the incorporation of up to 20% (v/v) oxygen in the hydrogen used for gassing. The stimulatory effect of oxygen was prevented by the addition of 10% (v/v) CO2 to ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00690224
更新日期：1976-11-02 00:00:00
abstract：:The determination of enzymatic activities in cell-free extracts of Acidaminococcus fermentans and Peptostreptococcus asaccharolyticus led to a refined scheme for the pathway of glutamate fermentation via (R)-2-hydroxyglutarate to acetate and butyrate. From the ratio of these products the amount of ATP generated by sub...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447055
更新日期：1985-07-01 00:00:00
abstract：:A strictly anaerobic bacterium, strain PCE1, was isolated from a tetrachloroethene-dechlorinating enrichment culture. Cells of the bacterium were motile curved rods, with approximately four lateral flagella. They possessed a gram-positive type of cell wall and contained cytochrome c. Optimum growth occurred at pH 7.2-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050308
更新日期：1996-02-01 00:00:00
