Abstract：
:The major cobamide-containing protein from methanol-utilizing Sporomusa ovata was 8-fold enriched to apparent homogeneity. The protein exhibited a molecular mass of 40 kDa and of 38 kDa determined by gel filtration and by SDS-polyacrylamide gel electrophoresis, respectively. This finding indicates a monomeric protein structure. Monospecific polyclonal antisera raised against the protein did not cross react with another cobamide-containing protein from Sporomusa cells. Only the 40 kDa cobamide-containing protein was induced by methanol, since proteins from cells grown on 3,4-dimethoxybenzoate, betaine H2/CO2, or fructose showed faint or no cross reaction. Hence, the 40 kDa cobamide-containing protein is presumably involved in the methyl-transfer reaction of the methanol metabolism. The purified enzyme revealed 1.1 mol of p-cresolyl cobamide per mol of protein, but it lacked of iron-sulfur centers. Remarkably, the cofactor was firmly bound to its protein.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Stupperich E,Aulkemeyer P,Eckerskorn Cdoi
10.1007/BF00245367keywords:
subject
Has Abstractpub_date
1992-01-01 00:00:00pages
370-3issue
5eissn
0302-8933issn
1432-072Xjournal_volume
158pub_type
杂志文章abstract：:An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 degrees C and 5.4 g NaCl l(-1). The strain grows heterotrophically on complex pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050745
更新日期：1999-08-01 00:00:00
abstract：:Stenotrophomonas maltophilia (Sm), with most of the isolates being resistant to multidrugs, is an opportunistic bacterium causing nosocomial infections. In this study, a novel virulent Sm phage, Smp14, was characterized. Electron microscopy showed that Smp14 resembled members of Myoviridae and adsorbed to poles of the...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0238-5
更新日期：2007-08-01 00:00:00
abstract：:Two gene clusters encoding similar formate dehydrogenases (FDH) were identified in Eubacterium acidaminophilum. Each cluster is composed of one gene coding for a catalytic subunit ( fdhA-I, fdhA-II) and one for an electron-transferring subunit ( fdhB-I, fdhB-II). Both fdhA genes contain a TGA codon for selenocysteine ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0508-1
更新日期：2003-01-01 00:00:00
abstract：:Gayal (Bos frontalis) of the Yunnan region is well adapted to harsh environmental conditions. Its diet consists predominantly of bamboo, reeds, and woody plants, suggesting that the rumen of this species contains many fiber-degrading bacteria and cellulases. The aim of this study was to identify and modify specific ce...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01812-3
更新日期：2020-07-01 00:00:00
abstract：:Gas chromatography-mass spectrometry studies of the nitrogen isotopic composition of the N-trifluoroacetyl n-butyl ester derivatives of the amino acids from isolated hydrolyzed cyanophycin from 15N-enriched cells led to two major findings: (1) the amino acid composition of this granular polypeptide, isolated using pro...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00314469
更新日期：1994-01-01 00:00:00
abstract：:Xanthobacter tagetidis grew as a chemolithotrophic autotroph on thiosulfate and other inorganic sulfur compounds, as a heterotroph on thiophene-2-carboxylic acid, acetic acid and alpha-ketoglutaric acid, and as a mixotroph on thiosulfate in combination with thiophene-2-carboxylic acid and/or acetic acid. Autotrophic g...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050568
更新日期：1998-03-01 00:00:00
abstract：:A LD-carboxypeptidase from Escherichia coli K 12 was isolated by Tris-EDTA treatment and purified to electrophoretic homogeneity by DEAE-cellulose chromatography. The enzyme has a molecular weight of approximately 12,000 as determined by sodium dodecyl sulfate-polyacrylamide electrophoresis and by Sephadex G-100 gel f...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00414732
更新日期：1986-03-01 00:00:00
abstract：:The endophytic diazotrophic Gluconacetobacter diazotrophicus PAL5 was originally isolated from sugarcane (Saccharum officinarum). The biological nitrogen fixation, phytohormones secretion, solubilization of mineral nutrients and phytopathogen antagonism allow its classification as a plant growth-promoting bacterium. T...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0794-1
更新日期：2012-07-01 00:00:00
abstract：:The ability of the imperfect soil fungus Paecilomyces lilacinus to transform the environmental pollutant dibenzofuran was investigated. Transformation of dibenzofuran and related derivatives lead to 14 products, which were identified by UV spectroscopy, mass spectrometry, and proton nuclear magnetic resonance spectros...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0695-z
更新日期：2004-09-01 00:00:00
abstract：:The alpha-glucosidase (alpha-D-glucoside glucohydrolase, EC 3.2.1.20) of Pseudomonas fluorescens W was partially purified by (NH4)2SO4 fractionation, Sephadex G-200 and DEAE-cellulose column chromatography. The enzyme showed great specificity for maltose hydrolysis, with very little action against polymeric forms. Suc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425338
更新日期：1976-04-01 00:00:00
abstract：:In order to obtain strains of Saccharomycopsis lipolytica impaired in the active transport of L-lysine, mutants resistant to a mixture of L-canavanine, L-4-5-transdehydrolysine and L-S-amino ethylcysteine, taken either all three or two by two, were isolated. These compounds were shown previously to be competitive inhi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00411361
更新日期：1979-08-06 00:00:00
abstract：:Gene probe hybridization was used to determine distribution and expression of co-metabolic genes at a contaminated site as it underwent in situ methanotrophic bioremediation of trichloroethylene (TCE). The bioremediation strategies tested included a series of air, air:methane, and air:methane:nutrient pulses of the te...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0445-8
更新日期：2009-03-01 00:00:00
abstract：:We explored the molecular diversity of cytochrome P450 genes in the filamentous fungus Aspergillus oryzae using bioinformatic and experimental approaches. Based on bioinformatic annotation, we found 155 putative genes of cytochromes P450 in the whole genome sequence; however, 13 of 155 appeared to be pseudogenes due t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0562-z
更新日期：2010-05-01 00:00:00
abstract：:The biosynthesis of two flavoproteins, 6-hydroxy-D-nicotine oxidase with covalently bound FAD and 6-hydroxy-L-nicotine oxidase containing non-covalently bound FAD, was studied in wild-type cells and in a riboflavin-requiring mutant of Arthrobacter oxidans. In the mutant cells, the rate of synthesis and the maximal act...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00407929
更新日期：1978-10-04 00:00:00
abstract：:Bacteriochlorophyll (BChl) c in whole cells of Chlorobium tepidum grown at 46 degrees C changed into bacteriopheophytin (BPhe) c within 10 days after reaching full growth. When a small amount of C. tepidum cells in which BChl c had been completely pheophytinized were transferred to a new culture medium, normal growth ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050737
更新日期：1999-07-01 00:00:00
abstract：:Essential oils (EOs) are products of plant origin and include mixtures of different chemical compounds. These volatile substances have many interesting properties, including antifungal properties. Fungi may develop under field conditions on crops such as wheat or corn and are able to synthesize mycotoxins, which adver...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01673-5
更新日期：2019-10-01 00:00:00
abstract：:A complex polydispersity became apparent when the poly(glycerophosphate) lipoteichoic acid of Enterococcus faecalis was chromatographed on DEAE-Sephadex. The chain length varied between 13 and 33 glycerophosphate residues per lipid anchor. In parallel, the extent of chain glycosylation increased from 0.2 to 0.4 digluc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00249103
更新日期：1992-01-01 00:00:00
abstract：:Two intestinal bacterial strains MT4s-5 and MT42 involved in the degradation of (-)-epigallocatechin (EGC) were isolated from rat feces. Strain MT4s-5 was tentatively identified as Adlercreutzia equolifaciens. This strain converted EGC into not only 1-(3, 4, 5-trihydroxyphenyl)-3-(2, 4, 6-trihydroxyphenyl)propan-2-ol ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-1006-y
更新日期：2014-10-01 00:00:00
abstract：:During a screening program intended to identify genes encoding enzymes typical for secondary metabolism in Sorangium cellulosum So ce90, an aromatic amino acid decarboxylase gene (ddc) was detected. Expression of ddc in Escherichia coli and subsequent enzyme assays with cell-free extracts confirmed the proposed functi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002039900131
更新日期：2000-04-01 00:00:00
abstract：:Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants virus was studied in Vero cells to determine maximum virus yield. One-step growth curve was formulated after determining virus in both supernatant (CFV) and cell lysate (CAV) at different time categories by microtitre plate titrat...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02154-w
更新日期：2021-01-05 00:00:00
abstract：:Bacillus cereus ATCC 14579 transformed naphthalene predominately to 1-naphthol. Experiments with [14C]naphthalene showed that over a 24 h period, B. cereus oxidized 5.2% of the added naphthalene. 1-Naphthol accounted for approximately 80% of the total metabolites. B. cereus incubated with naphthalene under the presenc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00410891
更新日期：1984-08-01 00:00:00
abstract：:Acinetobacter strain IVS-B aerobically grows on isovalerate as sole carbon and energy source. Isovalerate is metabolised via isovaleryl-CoA, an intermediate of the oxidative (S)-leucine degradation pathway. A 3-methylglutaconyl-CoA hydratase (EC 4.2.1.18) was purified 65-fold to apparent homogeneity from cell-free ext...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0095-7
更新日期：2006-05-01 00:00:00
abstract：:During the course of isolating rare actinobacteria from unexplored habitats, strain CH32T was obtained from an arid soil sample in eastern Anatolia, Turkey. Polyphasic characterization and comprehensive genome analyses showed that the strain is a member of the genus Nonomuraea and it is closely related to Nonomuraea g...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01941-9
更新日期：2020-10-01 00:00:00
abstract：:Twelve genes involved in thiamin biosynthesis in prokaryotes have been identified and overexpressed. Of these, six are required for the thiazole biosynthesis (thiFSGH, thil, and dxs), one is involved in the pyrimidine biosynthesis (thiC), one is required for the linking of the thiazole and the pyrimidine (thiE), and f...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s002030050713
更新日期：1999-04-01 00:00:00
abstract：:Respiratory NADH oxidation in the rumen bacterium Prevotella bryantii is catalyzed by the Na+-translocating NADH:quinone oxidoreductase (NQR). A method for cell disruption and membrane isolation of P. bryantii under anoxic conditions using the EmulisFlex-C3 homogenizer is described. We compared NQR activity and protei...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01805-x
更新日期：2020-07-01 00:00:00
abstract：:To address correlations between population sizes of microbes on the leaf surfaces and leaf morphological and physicochemical properties, various leaf morphological and physicochemical features as possible predictors of microbial population sizes on the leaf surfaces of four Napier grass cultivars were assessed. Result...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02025-4
更新日期：2021-01-01 00:00:00
abstract：:Microorganisms have evolved themselves to thrive under various extreme environmental conditions such as extremely high or low temperature, alkalinity, and salinity. These microorganisms adapted several metabolic processes to survive and reproduce efficiently under such extreme environments. As the major proportion of ...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-018-1602-3
更新日期：2019-01-01 00:00:00
abstract：:The ability of Klebsiella oxytoca NRRL-B199 to use either lactose or the mixture of glucose and galactose as substrate for the production of 2,3-butanediol was studied in batch fermentations with different conditions of aeration and pH. 2,3-butanediol was undetected, or present in minute concentration in the fermentat...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446920
更新日期：1989-01-01 00:00:00
abstract：:A monoclonal antibody obtained by immunization of mice with heat-killed cells of Listeria monocytogenes serotype 4d showed reactivity towards a protein (P45) from L. monocytogenes with an apparent molecular mass of 45 kDa. This protein was detected in the culture supernatant and at the cell surface of L. monocytogenes...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050003
更新日期：2000-01-01 00:00:00
abstract：:Autotrophic cultures of the facultative chemolithotroph Alcaligenes eutrophus have been found to excrete glycollate. This excretion was greatly stimulated by the incorporation of up to 20% (v/v) oxygen in the hydrogen used for gassing. The stimulatory effect of oxygen was prevented by the addition of 10% (v/v) CO2 to ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00690224
更新日期：1976-11-02 00:00:00
