Abstract:
:Gas chromatography-mass spectrometry studies of the nitrogen isotopic composition of the N-trifluoroacetyl n-butyl ester derivatives of the amino acids from isolated hydrolyzed cyanophycin from 15N-enriched cells led to two major findings: (1) the amino acid composition of this granular polypeptide, isolated using procedures optimized for extracting and purifying cyanophycin from cells in the stationary growth phase, varied with the culture growth condition; (2) the rate of incorporation of exogenous nitrate differed for each nitrogen atom of the amino acid constituents of cyanophycin or cyanophycin-like polypeptide. Arginine and aspartic acid were the principle components of cyanophycin isolated from exponentially growing cells and from light-limited stationary phase cells, with glutamic acid as an additional minor component. The cyanophycin-like polypeptide from nitrogen-limited cells contained only aspartic and glutamic acids, but no arginine. The glutamic acid content decreased and arginine content increased as nitrate was provided to nitrogen-limited cells. These cells rapidly incorporated nitrate at different rates at each cyanophycin nitrogen site: guanidino nitrogens of arginine > aspartic acid > alpha-amino nitrogen of arginine > glutamic acid. Little media-derived nitrogen was incorporated into cyanophycin of exponentially growing cells during one cellular doubling time.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Merritt MV,Sid SS,Mesh L,Allen MMdoi
10.1007/BF00314469subject
Has Abstractpub_date
1994-01-01 00:00:00pages
158-66issue
3eissn
0302-8933issn
1432-072Xjournal_volume
162pub_type
杂志文章abstract::Curcumin is a yellow-orange compound derived from the root of Curcuma longa (Zingiberaceae family), that has been used as a medicine, spice and coloring agent. Curcumin has proved nontoxic in a number of cell culture and whole animal studies. Curcumin has, however, been reported to have bactericidal effects at very hi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00414437
更新日期:1989-01-01 00:00:00
abstract::Carotenogenic mutants of Corynebacterium glutamicum were analyzed for their carotenoid content. Mutant MV10 accumulated the same carotenoids as the wild-type, decaprenoxanthin, decaprenoxanthin monoglucoside, and (2R,6R,2'R,6'R)-decaprenoxanthin di-(beta-D)-glucoside, but in three-fold higher amounts. In addition, dec...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030100315
更新日期:2001-09-01 00:00:00
abstract::ModE protein, a molybdate sensor/regulator, controls the transcription of genes coding for molybdate uptake (mod), molybdopterin synthesis (moa), molybdoenzymes nitrate reductase (nap) and dimethylsulfoxide reductase (dms), as well as fermentative dihydrogen production (fdhF and hyc) and respiratory nitrate reductase ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0039-7
更新日期:2005-12-01 00:00:00
abstract::In autotrophic methanogens, pyruvate oxidoreductase (POR) plays a key role in the assimilation of CO(2) and the biosynthesis of organic carbon. This enzyme has been purified to homogeneity, and the genes from Methanococcus maripaludis were sequenced. The purified POR contained five polypeptides with molecular masses o...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0554-3
更新日期:2003-06-01 00:00:00
abstract::The family Chlamydiaceae contains nine species pathogenic to humans and animals, but their routine identification is hampered by inadequate detection methods. In an attempt to find a new region for PCR detection and discrimination of the Chlamydiaceae species, the 3' end of the omp2 gene of Chlamydiaceae has been exam...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0757-2
更新日期:2005-03-01 00:00:00
abstract::N-Formylmaleamic acid, a probable intermediate in the bacterial metabolism of nicotinic acid, has been synthesized by photoisomerization of its trans isomer, N-formylfumaramic acid. The compound previously reported to be N-formylmaleamic acid has been shown to be N-formylfumaramic acid. ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00416972
更新日期:1976-10-11 00:00:00
abstract::Stenotrophomonas maltophilia (Sm), with most of the isolates being resistant to multidrugs, is an opportunistic bacterium causing nosocomial infections. In this study, a novel virulent Sm phage, Smp14, was characterized. Electron microscopy showed that Smp14 resembled members of Myoviridae and adsorbed to poles of the...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0238-5
更新日期:2007-08-01 00:00:00
abstract::The thermophilic bacterium Thermoanaerobacter tengcongensis has two single-stranded DNA-binding (SSB) proteins, designated TteSSB2 and TteSSB3. In a SSB complementation assay in Escherichia coli, only TteSSB3 took over the in vivo function of EcoSSB. We have cloned the ssb genes obtained by PCR and have developed E. c...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-008-0366-6
更新日期:2008-07-01 00:00:00
abstract::Bacillus cereus ATCC 14579 transformed naphthalene predominately to 1-naphthol. Experiments with [14C]naphthalene showed that over a 24 h period, B. cereus oxidized 5.2% of the added naphthalene. 1-Naphthol accounted for approximately 80% of the total metabolites. B. cereus incubated with naphthalene under the presenc...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00410891
更新日期:1984-08-01 00:00:00
abstract::Recently, it was proposed that some antibiotics stimulate the production of reactive oxygen species (ROS), which contribute to cell death. Later, other research groups have provided arguments against ROS-mediated killing of bacteria by antibiotics. At present, there remain a number of unanswered questions in understan...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1247-z
更新日期:2016-11-01 00:00:00
abstract::Of 147 microorganisms isolated from a loamy sand, 71 showed good growth with lindane (gamma-1,2,3,4,5,6-hexachlorocyclohexane) and produced chloride in an aqueous medium. Thirteen soil microorganisms were selected to study the utilization of lindane. Lindane was metabolized by the microbes to gamma-2,3,4,5,6-pentachlo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00454850
更新日期:1976-07-01 00:00:00
abstract::Microbial flavohaemoglobins are proteins with homology to haemoglobins from higher organisms, but clearly linked to nitric oxide (NO) metabolism by bacteria and yeast. hmp mutant strains of several bacteria are hypersensitive to NO and related compounds and hmp genes are up-regulated by the presence of NO. The regulat...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-006-0175-8
更新日期:2007-01-01 00:00:00
abstract::A novel thermotolerant strain of the achlorophyllous micro-alga Prototheca was isolated from a hot spring. The isolate was found to produce an appreciable amount of ethanol and CO2 from glucose under anoxic conditions at both 25 and 40 degrees C; this type of alcohol fermentation has not yet been reported in the genus...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-001-0384-0
更新日期:2002-03-01 00:00:00
abstract::A novel gram-negative, aerobic, non-spore-forming, rod-shaped and non-nitrogen fixing bacterium named strain 24NRT was isolated from wild Lilium pumilum bulbs in Fuping, Baoding City, Hebei province, PR China. The 16S rRNA gene sequences of strains 24NRT showed the highest similarity to Neorhizobium alkalisoli DSM 218...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01774-1
更新日期:2020-04-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02122-4
更新日期:2021-01-02 00:00:00
abstract::The characteristics of an in vitro polyuridylic acid dependent amino acid incorporating system prepared from germinating macroconidia of Microsporum canis are described. The incorporation of 14C-phenylalanine into polyphenylalanine is dependent on S-30 extract, adenosine triphosphate, magnesium ions and polyuridylic a...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00413276
更新日期:1979-01-16 00:00:00
abstract::The disaccharide sophorose induces Trichoderma to increase a soluble intracellular b-glucosidase that hydrolyses cellobiose, sophorose, and p-nitrophenyl-b-D-glucopyranoside. Simultaneously, it depresses the activity of a similar insoluble enzyme that is associated with the mycelium. Gel electrophoresis indicates that...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425807
更新日期:1984-01-01 00:00:00
abstract::Expression of the gene encoding ribosome modulation factor (RMF), as measured using an rmf-lacZ gene fusion, increased with decreasing pH in exponential phase cultures of Escherichia coli. Expression was inversely proportional to the growth rate and independent of the acidifying agent used and it was concluded that ex...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0025-0
更新日期:2005-10-01 00:00:00
abstract::We report the phylogenetic and physiological characterization of a mesophilic and halophilic member of the filamentous anoxygenic phototrophic (FAP) bacteria, provisionally named ' Candidatus Chorothrix halophila' gen. nov. sp. nov., that has been maintained in a highly enriched culture in our laboratory for over a de...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0615-7
更新日期:2004-01-01 00:00:00
abstract::The propensity of bacterium to sporulate or retain the vegetative form depends on the amount of phosphorylated Spo0A (Spo0A(-P)), regulated by Spo0E multigene family of phosphatases (Spo0E, YisI and YnzD). Phylogenetic analysis revealed that Spo0E multigene family of phosphatases (SMFP) descends in two distinct clades...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-008-0446-7
更新日期:2009-03-01 00:00:00
abstract::Two different acidic polysaccharides (I and II) were detected in the external cell envelope layers (slime and capsule) of Rhodopseudomonas capsulata Sp11. Polysaccharide I contains rhamnose, fucose, glucosamine and an unknown acidic sugar, it represents the slime material of the strain. Polysaccharide II contains rham...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425219
更新日期:1983-12-01 00:00:00
abstract::Streptococcus intermedius is a causative agent of brain or liver abscesses. S. intermedius produces intermedilysin that plays a pivotal role in pathogenicity. We identified other pathogenic factors and described a fibronectin binding protein (FBP) homolog of S. intermedius (FbpI) that mediated bacterial adhesion to ep...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01922-y
更新日期:2020-10-01 00:00:00
abstract::Mannoproteins from cell walls of Saccharomyces cerevisiae synthesized at successive stages of the population growth cycle have been solubilized with Zymolyase and subsequently analyzed. The major change along the population cycle concerned a large size mannoprotein material; the size of the newly-synthesized molecules...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425354
更新日期:1987-07-01 00:00:00
abstract::The transport of branched-chain amino acids was characterized in intact cells of Corynebacterium glutamicum ATCC 13032. Uptake and accumulation of these amino acids occur via a common specific carrier with slightly different affinities for each substrate (Km[Ile] = 5.4 microM, Km[Leu] = 9.0 microM, Km[Val] = 9.5 micro...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00413136
更新日期:1989-01-01 00:00:00
abstract::Autotrophic cultures of the facultative chemolithotroph Alcaligenes eutrophus have been found to excrete glycollate. This excretion was greatly stimulated by the incorporation of up to 20% (v/v) oxygen in the hydrogen used for gassing. The stimulatory effect of oxygen was prevented by the addition of 10% (v/v) CO2 to ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00690224
更新日期:1976-11-02 00:00:00
abstract::The fast-growing Rhizobium sp. strain NGR234, isolated from Papua New Guinea, and 13 strains of Sinorhizobium fredii, isolated from China and Vietnam, were fingerprinted by means of RAPD, REP, ERIC and ARDRA. ERIC, REP and RAPD markers revealed a considerable genetic diversity among fast-growing rhizobia. Chinese isol...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0559-y
更新日期:2003-07-01 00:00:00
abstract::In this study, two quorum sensing (QS) system genes, las and rhI; N-3-oxo-dodecanoyl homoserine lactone (AHL; 3-O-C12-HSL); and QS-related virulence factors and correlation between them were assessed in 30 fish origin P. aeruginosa isolates. The detection of two QS system of the isolates, and eight gene regions consis...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02159-5
更新日期:2021-01-04 00:00:00
abstract::In order to obtain strains of Saccharomycopsis lipolytica impaired in the active transport of L-lysine, mutants resistant to a mixture of L-canavanine, L-4-5-transdehydrolysine and L-S-amino ethylcysteine, taken either all three or two by two, were isolated. These compounds were shown previously to be competitive inhi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00411361
更新日期:1979-08-06 00:00:00
abstract::The attachment of four species of bacteria to solid surfaces was investigated to determine whether the attachment of one species of bacterium could be influenced by the presence of other attaching or attached species. Three types of experiment were done: attachment of bacteria from suspensions containing two species (...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00429648
更新日期:1987-06-01 00:00:00
abstract::Coagulase-negative staphylococci (CNS) are opportunistic pathogens that are currently emerging as causative agents of human disease. Though CNS are widespread in the clinic and food, their precise identification at species level is important. Here, using 16S rRNA sequencing, 55 staphylococcal isolates were identified ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-017-1415-9
更新日期:2018-01-01 00:00:00