Abstract：
:The biosynthesis of two flavoproteins, 6-hydroxy-D-nicotine oxidase with covalently bound FAD and 6-hydroxy-L-nicotine oxidase containing non-covalently bound FAD, was studied in wild-type cells and in a riboflavin-requiring mutant of Arthrobacter oxidans. In the mutant cells, the rate of synthesis and the maximal activity level of both enzymes after induction by nicotine depended on the amount of added riboflavin. The low rate of synthesis in the presence of 2 micron riboflavin could be enhanced during the induction phase by further addition of riboflavin (33 micron). Inhibitors of translation (chloramphenicol or streptomycin) completely blocked the synthesis of both flavoproteins. Inhibitors of transcription (rifamycin S or actinomycin D) stopped the synthesis of both enantiozymes in wild-type cells and in the mutant grown in the presence of a saturating supply of riboflavin (15 micron). Under conditions of restricted flavoprotein synthesis (2 micron riboflavin in the medium), however, the mutant cells continued to synthesize the enzyme for 2--3 h after the addition of the transcription inhibitors. It appears, that in these cells a rather stable m-RNA accumulated during riboflavin-limited flavoprotein synthesis. The dependence of the effect of transcription inhibitors on the extracellular supply of riboflavin suggests that the regulation of the synthesis of both flavoproteins occurs not only by control of gene expression (induction by nicotine), but also at the level of translation through the availability of FAD.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Hamm HH,Decker Kdoi
10.1007/BF00407929subject
Has Abstractpub_date
1978-10-04 00:00:00pages
65-70issue
1eissn
0302-8933issn
1432-072Xjournal_volume
119pub_type
杂志文章abstract：:Bacillus thuringiensis (Bt) is efficient, strongly specific, and avirulent to humans, making it one of the most popular biopesticides in the world. Bt LLP29 is a mosquitocidal strain that was first isolated from Magnolia denudata. To understand its molecular mechanism against mosquitoes, the genome of Bt LLP29 was seq...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01875-2
更新日期：2020-09-01 00:00:00
abstract：:Reciprocal qualitative and quantitative immunological experiments employing an anti-Pediococcus cerevisiae aldolase serum confirmed many of the interspecific relationships demonstrated previously among lactic acid bacteria with antisera prepared against the Streptococcus faecalis fructose diphosphate aldolase. The ext...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00416976
更新日期：1976-10-11 00:00:00
abstract：:Geobacter species such as G. bremensis, G. pelophilus, and G. sulfurreducens are obligately anaerobic and grow in anoxic, non-reduced medium by fast reduction of soluble ferric citrate. In contrast, insoluble ferrihydrite was either only slowly or not reduced when supplied as electron acceptor in similar growth experi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0686-0
更新日期：2004-10-01 00:00:00
abstract：:Cell free extracts prepared from exponentially growing Escherichia coli and Bacillus cereus as well as from Bacillus cereus at the end of exponential growth were optimized for various factors required for amino acid incorporation when programmed with Bacillus ribonucleic acid. All three preparations synthesized glutam...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00455354
更新日期：1981-04-01 00:00:00
abstract：:To analyze if chemical cell wall alterations contribute to penicillin-induced bacteriolysis, changes in the amount, stability, and chemical composition of staphylococcal cell walls were investigated. All analyses were performed before onset of bacteriolysis i.e. during the first 60 min following addition of different ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00249181
更新日期：1990-01-01 00:00:00
abstract：:The haloalkaliphilic archaeon Natronobacterium vacuolatum forms cylinder-shaped gas vesicles throughout the growth cycle when grown in media containing 15-25% NaCl. Cells cultivated in media containing 13% NaCl are, however, gas-vesicle-free. The major gas vesicle structural protein, nv-GvpA, was detected by an antise...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050465
更新日期：1997-07-01 00:00:00
abstract：:Aquaspirillum magnetotacticum MS-1 cells cultured microaerobically (dissolved O2 tension 1% of saturation), expressed proteins with superoxide dismutase (SOD) activity. The majority (roughly 95%) of total cell superoxide dismutase activity was located in the cell periplasm with little or no activity in the cell cytopl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425171
更新日期：1989-01-01 00:00:00
abstract：:Purple sulfur bacterium Thiocapsa roseopersicina strain BBS requiring vitamin B12 may grow in the dark in media containing no other organic compounds. Under such conditions the cells oxidize sulfide and thiosulfate with the use of O2 and assimilate carbon dioxide. After 10--30s assimilation of NaH14CO3 about 60% of ra...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454854
更新日期：1976-07-01 00:00:00
abstract：:Two proteases, designated I and II, have been isolated from sporulating cells of Bacillus subtilis. They were partially purified by ammonium sulfate fractionation, Sephadex chromatography and affinity columns. Protease I was found to be similar to an already characterized B. subtilis protease. Protease II is trypsin-l...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425256
更新日期：1981-05-01 00:00:00
abstract：:Enoate reductase present in Clostridium kluyveri and Clostridium spec. La 1 could be detected in three strains of C. tyrobutyricum and ten clostridia belonging to the groups of proteolytic and saccharolytic or proteolytic species, respectively. In C. pasteurianum, C. butyricum and C. propionicum enoate reductase could...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00419482
更新日期：1983-08-01 00:00:00
abstract：:Recently, we reported the synthesis of five bacteriocin-like inhibitor substances (Bt-BLIS: morricin 269, kurstacin 287, kenyacin 404, entomocin 420, and tolworthcin 524) by Mexican strains of Bacillus thuringiensis. Here we show that, collectively, these Bt-BLIS have a moderate to broad spectrum of antibacterial acti...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0414-2
更新日期：2008-12-01 00:00:00
abstract：:A Gram reaction-negative, strictly aerobic, non-motile, translucent and rod-shaped bacterium (designated W1-2-4(T)) isolated from tap water was characterized by a polyphasic approach to clarify its taxonomic position. Strain W1-2-4(T) was observed to grow optimally at 25-30 °C and at pH 6.5 on nutrient agar. Phylogene...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1237-1
更新日期：2016-10-01 00:00:00
abstract：:The metabolism of methylamine as the nitrogen source for growth of the non-methylotrophic yeast Candida utilis and the methylotrophic yeast Hansenula polymorpha was investigated. Growth of both organisms in media with glucose and methylamine was associated with the presence of an amine oxidase in these cells. The enzy...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00511216
更新日期：1980-06-01 00:00:00
abstract：:In beta-lactam-antibiotic-producing fungi, such as Aspergillus (Emericella) nidulans, L-alpha-aminoadipic acid is the branching point of the lysine and penicillin biosynthesis pathways. To obtain a deeper insight into the regulation of lysine biosynthesis genes, the regulation of the A. nidulans lysF gene, which encod...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030000246
更新日期：2001-02-01 00:00:00
abstract：:The ability of the imperfect soil fungus Paecilomyces lilacinus to transform the environmental pollutant dibenzofuran was investigated. Transformation of dibenzofuran and related derivatives lead to 14 products, which were identified by UV spectroscopy, mass spectrometry, and proton nuclear magnetic resonance spectros...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0695-z
更新日期：2004-09-01 00:00:00
abstract：:An alkaliphilic actinobacterial strain, designated Hz 6-5T, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China. The isolate formed light yellow-colored colonies and its cells were Gram-staining positive, non-motile, and non-spore-forming short rods. The strain was aerobic with optimal gro...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02073-w
更新日期：2020-10-26 00:00:00
abstract：:A lack on the association between acid tolerance response (ATR) and osmotolerance response (OTR) among Listeria monocytogenes dairy isolates was found. In order to evaluate how wild L. monocytogenes isolates mount tolerance responses under a sub-lethal pH and a low sodium chloride concentration (pH 5.5 and 3.5 % [w/v]...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0878-6
更新日期：2013-05-01 00:00:00
abstract：:The gliding bacterium Herpetosiphon giganteus Hp a2 was shown to contain gamma-carotene, one monoglucosyloxy and two new diglucosyloxy carotenoids with a x-O-acyldiglucosyloxy carotenoid as main component. Flexirubin-like pigments could not be detected in this organism. As in the Myxobacterales and Cytophagales (Klein...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413098
更新日期：1977-04-01 00:00:00
abstract：:We report on the genome-wide response, based on DNA microarrays, of the cyanobacterium Synechocystis sp. PCC 6803 wild type and DeltasigB to a 15 min heat shock. Approximately 9% of the genes in wild type and DeltasigB were significantly regulated (P < 0.001) following this treatment, with chaperones induced the most....
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0138-0
更新日期：2006-10-01 00:00:00
abstract：:Bacillus cereus ATCC 14579 transformed naphthalene predominately to 1-naphthol. Experiments with [14C]naphthalene showed that over a 24 h period, B. cereus oxidized 5.2% of the added naphthalene. 1-Naphthol accounted for approximately 80% of the total metabolites. B. cereus incubated with naphthalene under the presenc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00410891
更新日期：1984-08-01 00:00:00
abstract：:An Arthrobacter sp. growing on 4-Chlorobenzoic acid as its sole source of carbon excretes 4-hydroxygenzoic acid and protocatechuic acid into the culture medium. Protocatechuic acid is further attacked by "meta"-cleavage. During growth of the Arthrobacter sp. on benzoic acid cis-cis muconic acid can be isolated from th...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00690235
更新日期：1976-11-02 00:00:00
abstract：:Sulfolane (tetrahydrothiophene-1,1-dioxide) is used in the Sulfinol process for natural gas sweetening. At many sour-gas processing plants spills, landfills and leakage from unlined surface storage ponds have contaminated groundwaters with sulfolane. Due to its high water solubility and mobility in aquifers, sulfolane...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030000184
更新日期：2000-07-01 00:00:00
abstract：:The divergently transcribed sulfur oxidation (sox) operon of a sulfur chemolithotrophs, Pseudaminobacter salicylatoxidans KCT001, comprising sox TRS-VW-XYZABCD, is regulated by a repressor (SoxR). SoxR binds to two disparate operators, sv (present in between soxS and soxV) and wx (present in between soxW and soxX). He...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0808-z
更新日期：2012-09-01 00:00:00
abstract：:Streptococcus intermedius is a causative agent of brain or liver abscesses. S. intermedius produces intermedilysin that plays a pivotal role in pathogenicity. We identified other pathogenic factors and described a fibronectin binding protein (FBP) homolog of S. intermedius (FbpI) that mediated bacterial adhesion to ep...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01922-y
更新日期：2020-10-01 00:00:00
abstract：:We previously cloned the sigH gene encoding a stress-response sigma factor sigma(H) in Streptomyces coelicolor A3(2), located in an operon with the gene encoding proposed anti-sigma factor UshX. To clarify the in vivo function of sigma(H), a stable null mutant of sigH was prepared by homologous recombination. This mut...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-001-0367-1
更新日期：2001-12-01 00:00:00
abstract：:Selenium nanoparticles (Se NPs) were synthesized using Saccharomyces cerevisiae yeast. Influences of different amounts of sodium selenite (5.0, 10.0, 15.0, 20.0, and 25 µg) were evaluated on growth of yeast during incubation at 32 °C, during 4 days. UV-Vis spectroscopy results have shown that synthesized Se NPs had br...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01831-0
更新日期：2020-07-01 00:00:00
abstract：:The L-alanine mediated germination of food isolated Bacillus cereus DSA 1 spores, which lacked an intact exosporium, increased in the presence of D-cycloserine (DCS), which is an alanine racemase (Alr) inhibitor, reflecting the activity of the Alr enzyme, capable of converting L-alanine to the germination inhibitor D-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0946-y
更新日期：2014-02-01 00:00:00
abstract：:In order to investigate the role of the putative epimerase function of the beta-oxidation multienzyme complex (FadBA) in the provision of (R)-3-hydroxyacyl-CoA thioesters for medium-chain-length polyhydroxyalkanoate (PHA(MCL)) biosynthesis, the fadBA(Po) operon of Pseudomonas oleovorans was cloned and characterized. T...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0444-0
更新日期：2002-08-01 00:00:00
abstract：:We investigated the structural and immunochemical characteristics of cell wall mannan obtained from Candida sojae JCM 1644, which is a new yeast species isolated from defatted soybean flakes. The results of a slide-agglutination test and of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0339-1
更新日期：2008-05-01 00:00:00
abstract：:An anaerobic, mesophilic, syntrophic benzoate-degrading bacterium, designated strain FB(T), was isolated from methanogenic sludge which had been used to treat wastewater from the manufacture of terephthalic acid. Cells were non-motile gram-positive rods that formed spores. The optimum temperature for growth was 35-40 ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0521-z
更新日期：2003-04-01 00:00:00
