Identification of an L-dopa decarboxylase gene from Sorangium cellulosum So ce90.

Abstract:

:During a screening program intended to identify genes encoding enzymes typical for secondary metabolism in Sorangium cellulosum So ce90, an aromatic amino acid decarboxylase gene (ddc) was detected. Expression of ddc in Escherichia coli and subsequent enzyme assays with cell-free extracts confirmed the proposed function derived from amino acid sequence comparisons. In contrast to other aromatic amino acid decarboxylases of eukaryotic origin, the S. cellulosum Ddc converted only L-dihydroxy phenylalanine. This is the first report of a gene encoding an L-dihydroxy phenylalanine decarboxylase in bacteria.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Müller R,Gerth K,Brandt P,Blöcker H,Beyer S

doi

10.1007/s002039900131

keywords:

subject

Has Abstract

pub_date

2000-04-01 00:00:00

pages

303-6

issue

4

eissn

0302-8933

issn

1432-072X

journal_volume

173

pub_type

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