Abstract：
:L-Alanine dehydrogenase was found in extracts of the antibiotic producer Streptomyces clavuligerus. The enzyme was induced by ammonia, and the level of induction was dependent on the extracellular concentraction. L-Alanine was the only amino acid able to induce alanine dehydrogenase. The enzyme was characterized from a 38-fold purified preparation. Pyruvate (Km = 1.1 mM), ammonia (Km = 20 mM) and NADH (Km = 0.14 mM) were required for the reductive amination, and L-alanine (Km = 9.1 mM) and NAD (Km = 0.5 mM) for the oxidative deaminating reaction. The aminating reaction was inhibited by alanine, serine and NADPH. Alanine inhibited uncompetitively with respect to NADH (Ki = 1.6 mM) and noncompetitively with respect to ammonia (Ki = 2.0 mM) and pyruvate (Ki = 3.0 mM). In the aminating reaction 3-hydroxypyruvate, glyoxylate and 2-oxobutyrate could partially (6--7%) substitute pyruvate. Alanine dehydrogenase from S. clavuligerus differed with respect to its molecular weight (92000) and its kinetic properties from those described for other microorganisms.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Aharonowitz Y,Friedrich CGdoi
10.1007/BF00403210subject
Has Abstractpub_date
1980-03-01 00:00:00pages
137-42issue
1-2eissn
0302-8933issn
1432-072Xjournal_volume
125pub_type
杂志文章abstract：:In recent years, bioremediation is considered as an efficient method to remove the pollutants from the industrial wastewater. In this study, quantitative gene expressions (Real-time RT-PCR) of mtr gene cluster (mtrA, mtrB, mtrC, mtrD, mtrE, mtrF and omcA) in five different uranium concentrations (0.1, 0.25, 0.5, 1 and...
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更新日期：2020-12-01 00:00:00
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journal_title：Archives of microbiology
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doi：10.1007/s00203-004-0719-8
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pub_type： 杂志文章
doi：10.1007/s00203-018-1493-3
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journal_title：Archives of microbiology
pub_type： 杂志文章
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245305
更新日期：1993-01-01 00:00:00
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journal_title：Archives of microbiology
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更新日期：1979-03-12 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
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更新日期：2000-05-01 00:00:00
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更新日期：1976-07-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00693397
更新日期：1985-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00422282
更新日期：1988-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00203-016-1213-9
更新日期：2016-08-01 00:00:00
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journal_title：Archives of microbiology
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更新日期：1995-12-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00249103
更新日期：1992-01-01 00:00:00
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更新日期：2017-12-01 00:00:00
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更新日期：2015-03-01 00:00:00
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更新日期：2006-06-01 00:00:00
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更新日期：1997-02-01 00:00:00
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更新日期：1997-04-01 00:00:00
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更新日期：1977-07-26 00:00:00
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更新日期：2009-03-01 00:00:00
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更新日期：2013-03-01 00:00:00
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更新日期：2002-06-01 00:00:00
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journal_title：Archives of microbiology
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更新日期：2006-04-01 00:00:00
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更新日期：1995-02-01 00:00:00
