Abstract:
:ModE protein, a molybdate sensor/regulator, controls the transcription of genes coding for molybdate uptake (mod), molybdopterin synthesis (moa), molybdoenzymes nitrate reductase (nap) and dimethylsulfoxide reductase (dms), as well as fermentative dihydrogen production (fdhF and hyc) and respiratory nitrate reductase (narXL) in Escherichia coli. The catalytic product of a second protein, MoeA, is also required for molybdate-dependent positive regulation of hyc and nar operons. To explore the potential role of ModE and MoeA in the regulation of other E. coli genes, the global gene expression profile of a wild type and a modE, moeA double mutant grown in glucose-minimal medium under anaerobic conditions were compared. Expression of 67 genes was affected by the modE and moeA mutations (P value <0.01). Of these, 17 differed by at least 2-fold or higher. Fourteen genes were expressed at a higher level in the mutant (2.4- to 23.9-fold) (notably, mod-molybdate transport, deo-nucleoside catabolism and opp-oligopeptide transport operons) and dmsA and yli operon were expressed at a higher level in the wild type parent (2.6- to 5.7-fold). One of the unexpected findings was repression of the deo operon by ModE. This was confirmed by quantitative RT-PCR and by the analysis of a deoC-lacZ fusion. The deo promoter/operator region contains a putative ModE-consensus sequence centered at -35 in which the adenines are replaced by guanines (TGTGT-N7-TGTGT). The ModE protein did bind to the deo upstream DNA and shifted its electrophoretic mobility. Bioinformatics analysis of the E. coli genome for ModE-consensus motif (TATAT-N7-TAYAT) identified 21 additional genes/operons including the moa as potential targets for Mo-control. The physiological role of many of the genes identified solely by bioinformatics (19/21) is unknown. Expression levels of these genes were similar in the parent and the isogenic modE, moeA mutant when cultured anaerobically in glucose-minimal medium. This study identified additional targets, such as deo and opp, for the Mo-dependent control in E. coli.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Tao H,Hasona A,Do PM,Ingram LO,Shanmugam KTdoi
10.1007/s00203-005-0039-7keywords:
subject
Has Abstractpub_date
2005-12-01 00:00:00pages
225-33issue
4eissn
0302-8933issn
1432-072Xjournal_volume
184pub_type
杂志文章abstract::The ability of the imperfect soil fungus Paecilomyces lilacinus to transform the environmental pollutant dibenzofuran was investigated. Transformation of dibenzofuran and related derivatives lead to 14 products, which were identified by UV spectroscopy, mass spectrometry, and proton nuclear magnetic resonance spectros...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0695-z
更新日期:2004-09-01 00:00:00
abstract::Xanthan-deficient mutants of Xanthomonas axonopodis pv. citri, the bacterium responsible for citrus canker, were generated by deletion and marker exchange of the region encoding the carboxy-terminal end of the first glycosyltransferase, GumD. Mutants of gumD did not produce xanthan and remained pathogenic in citrus pl...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0227-8
更新日期:2007-08-01 00:00:00
abstract::The objective of this work was to investigate the structure and diversity of lactic acid bacteria (LAB) communities in traditionally fermented meat collected from different areas of Tunisia. A polyphasic study, which involves phenotypic tests and ribosomal DNA-based techniques, was used to identify Gram-positive and c...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-009-0499-2
更新日期:2009-09-01 00:00:00
abstract::Upland rice is an ecotype crop resulting from the long-term domestication and evolution of rice in dry land without a water layer. Generally, the stems and leaves are thick and luxuriant, while the leaves also typically broad and light. The root system is developed with abundant root hair, and the osmotic pressure of ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02058-9
更新日期:2020-09-29 00:00:00
abstract::The diversity of eukaryotic populations, in particular protozoa, in the water supplies of intensively reared broilers has not been previously studied. This important food-rearing environment was screened for the molecular diversity of eukaryotes by the analysis of PCR-amplified 18S rRNA. DNA was extracted from filtere...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-006-0119-3
更新日期:2006-07-01 00:00:00
abstract::Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants virus was studied in Vero cells to determine maximum virus yield. One-step growth curve was formulated after determining virus in both supernatant (CFV) and cell lysate (CAV) at different time categories by microtitre plate titrat...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02154-w
更新日期:2021-01-05 00:00:00
abstract::Regulation of transcription of the Escherichia coli moe operon, which codes for proteins connecting molybdate metabolism, molybdopterin synthesis, and apomolybdoenzyme synthesis, was investigated. Expression of the moe operon was independent of genes coding for molybdate transport and Mo-cofactor biosynthesis. Express...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030100252
更新日期:2001-03-01 00:00:00
abstract::Pure cultures of an aerobically grown Flavobacterium sp. were shown by hydride generation-cold trap-atomic absorption spectrometry to biomethylate inorganic antimony (III) supplied as potassium antimony tartrate. Growth inhibition of the Flavobacterium sp. by antimony (III) over the range 0-30 mg Sb l(-1) was assessed...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0456-9
更新日期:2002-10-01 00:00:00
abstract::The present investigation deals with the optimization of cell growth rate of the candidate probiotic Pediococcus acidilactici in the presence of the specific prebiotic inulin. Three independent variables viz. concentration of inulin, concentration of glucose and pH have been selected for optimization study using respo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-015-1131-2
更新日期:2015-09-01 00:00:00
abstract::Calcium is essential for the growth of rhizobia and the formation of nitrogen-fixing root-nodules on legumes, but its precise role in these processes remains unknown. We have found that Rhizobium fredii USDA208 accumulates a major 38 kDa protein when grown in media supplemented with 0.3-2 microM Cacl2. We have purifie...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00248480
更新日期:1993-01-01 00:00:00
abstract::Cadmium is a widespread pollutant that has been associated with oxidative stress, but the mechanism behind this effect in prokaryotes is still unclear. In this work, we exposed two glutathione deficient mutants (DeltagshA and DeltagshB) and one respiration deficient mutant (DeltaubiE) to a sublethal concentration of c...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0316-8
更新日期:2008-03-01 00:00:00
abstract::A strictly anaerobic bacterium, strain PCE1, was isolated from a tetrachloroethene-dechlorinating enrichment culture. Cells of the bacterium were motile curved rods, with approximately four lateral flagella. They possessed a gram-positive type of cell wall and contained cytochrome c. Optimum growth occurred at pH 7.2-...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050308
更新日期:1996-02-01 00:00:00
abstract::The sulfate-reducing highly enriched culture N47 is capable to anaerobically degrade naphthalene, 2-methylnaphthalene, and 2-naphthoic acid. A proteogenomic investigation was performed to elucidate the initial activation reaction of anaerobic naphthalene degradation. This lead to the identification of an alpha-subunit...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-010-0667-4
更新日期:2011-04-01 00:00:00
abstract::All Serratia marcescens strains (total of 33) of different sources were hemolytic including clinical strains previously classified as being nonhemolytic. DNA fragments of the two hemolysin genes hybridized with the chromosomal DNA of S. marcescens, S. liquefaciens, S. kiliensis, S. grimesii, S. proteamaculans, S. plym...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00248958
更新日期:1990-01-01 00:00:00
abstract::The efficiency of hydrolysis of fungal (Fusarium spp.) cell wall and rye root cell wall by crude enzymatic complexes from (42-day-old) cultures of three F. culmorum isolates, a plant growth-promoting rhizosphere isolate (PGPF) DEMFc2, a deleterious rhizosphere isolate (DRMO) DEMFc5, and a pathogenic isolate DEMFc37, a...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-012-0803-4
更新日期:2012-08-01 00:00:00
abstract::NAD-linked lactate dehydrogenases specific for the D- and L-lactate have been demonstrated in a number of strains of unicellular cyanobacteria. The D-lactate dehydrogenase of one strain (Synechococcus 6716) was partially purified and its properties were studied. The enzyme has a molecular weight of ca. 115000-120000...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00447300
更新日期:1975-06-20 00:00:00
abstract::Helcococcus kunzii is a gram-positive, catalase-negative opportunist. The organism has been isolated from the lower extremities and breast masses of several patients. A clinical isolate of Helcococcus kunzii was shown to possess a hemagglutinin-lectin with a specificity for N-acetylglucosamine and lactose, two structu...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-001-0378-y
更新日期:2002-02-01 00:00:00
abstract::The signal orchestration between legumes and the rhizobia attribute to symbiotic nitrogen fixation through nodule formation. Root nodules serve as a nutrient-rich reservoir and harbor diverse microbial communities. However, the existence of non-rhizobial endophytes (NRE) and their role inside the root nodules are bein...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01983-z
更新日期:2020-12-01 00:00:00
abstract::The functional pmoA gene is frequently used to probe the diversity and phylogeny of methane-oxidizing bacteria (MOB) in various environments. Here, we compared the similarities between the pmoA gene and the corresponding 16S rRNA gene sequences of 77 described species covering gamma- and alphaproteobacterial methanotr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1222-8
更新日期:2016-09-01 00:00:00
abstract::A strain of Pseudomonas fluorescens was capable of co-metabolizing 3-chlorobenzoic acid with the production of a chlorinated catechol black pigment. A peroxidase and another enzymatic activity referred to as a polyphenol oxidase were found to be involved in the oxidation of 4-chlorocatechol to 4-chloro-1,2-benzoquinon...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00252220
更新日期:1993-01-01 00:00:00
abstract::Succinate:quinone reductase is a membrane-bound enzyme of the citric acid cycle and the respiratory chain. Carboxin is a potent inhibitor of the enzyme of certain organisms. The bacterium Paracoccus denitrificans was found to be sensitive to carboxin in vivo, and mutants that grow in the presence of 3'-methyl carboxin...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050611
更新日期:1998-07-01 00:00:00
abstract::Chlorella fusca, strain 211-15, cells degreened in a nitrogen-deficient mineral growth medium in the light for 4-6 weeks were regreened for up to 24 hrs in a nitrogen rich medium that leads to synchronous cell division at 24-26 hrs. Structural changes in the plastid membranes during the regreening period were observed...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00436359
更新日期:1975-05-05 00:00:00
abstract::In view of the functional similarities between subtilisin Carlsberg and the alkaline protease from Conidiobolus coronatus, the biochemical and structural properties of the two enzymes were compared. In spite of their similar biochemical properties, e.g., pH optima, heat stability, molecular mass, pI, esterase activity...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF01682989
更新日期:1996-12-01 00:00:00
abstract::Periodontal diseases are infectious polymicrobial inflammatory diseases that lead to destruction of the periodontal ligament, gingiva, and alveolar bone. Sequential colonization of a broad range of bacteria, including Fusobacterium nucleatum and Porphyromonas gingivalis, is an important phenomenon in this disease mode...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1223-7
更新日期:2016-08-01 00:00:00
abstract::To address correlations between population sizes of microbes on the leaf surfaces and leaf morphological and physicochemical properties, various leaf morphological and physicochemical features as possible predictors of microbial population sizes on the leaf surfaces of four Napier grass cultivars were assessed. Result...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02025-4
更新日期:2021-01-01 00:00:00
abstract::Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, one of the most widespread and destructive bacterial diseases in rice. This study identified and characterized the contribution of the twin-arginine translocation (Tat) pathway to motility, chemotaxis, extracellular polysaccharide (EPS) production and virulen...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-008-0440-0
更新日期:2009-02-01 00:00:00
abstract::Cellulomonas uda (DSM 20108/ATCC 21399) is one of the few described cellulolytic facultative anaerobes. Based on these characteristics, we initiated a physiological study of C. uda with the aim to exploit it for cellulase production in simple bioreactors with no or sporadic aeration. Growth, cellulase activity and fer...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1230-8
更新日期:2016-10-01 00:00:00
abstract::The control of pyrimidine nucleotide formation in the bacterium Pseudomonas aurantiaca ATCC 33663 by pyrimidines was studied. The activities of the pyrimidine biosynthetic pathway enzymes were investigated in P. aurantiaca ATCC 33663 cells and from cells of an auxotroph lacking orotate phosphoribosyltransferase activi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01842-x
更新日期:2020-08-01 00:00:00
abstract::The maleylacetate reductase of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 has been purified 50-fold. The enzyme converted 2-chloromaleylacetate to 3-oxoadipate with temporary occurrence of maleylacetate; 1 mol of chloride was eliminated during the conversion of 1 mol of 2-chloro- and 2,3-dichloromaleyl...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00276301
更新日期:1992-01-01 00:00:00
abstract::The Roseobacter clade, belonging to the family Rhodobacteraceae of the class Alphaproteobacteria, is one of the major bacterial groups in marine environments. A remarkable wealth of diverse large plasmids has been detected in members of this lineage. Here, we analysed the genome structure and extrachromosomal DNA cont...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-009-0535-2
更新日期:2010-02-01 00:00:00