Abstract：
:Cellulomonas uda (DSM 20108/ATCC 21399) is one of the few described cellulolytic facultative anaerobes. Based on these characteristics, we initiated a physiological study of C. uda with the aim to exploit it for cellulase production in simple bioreactors with no or sporadic aeration. Growth, cellulase activity and fermentation product formation were evaluated in different media under both aerobic and anaerobic conditions and in experiments where C. uda was exposed to alternating aerobic/anaerobic growth conditions. Here we show that C. uda behaves as a true facultative anaerobe when cultivated on soluble substrates such as glucose and cellobiose, but for reasons unknown cellulase activity is only induced under aerobic conditions on insoluble cellulosic substrates and not under anaerobic conditions. These findings enhance knowledge on the limited number of described facultative cellulolytic anaerobes, and in addition it greatly limits the utility of C. uda as an 'easy to handle' cellulase producer with low aeration demands.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Poulsen HV,Willink FW,Ingvorsen Kdoi
10.1007/s00203-016-1230-8subject
Has Abstractpub_date
2016-10-01 00:00:00pages
725-35issue
8eissn
0302-8933issn
1432-072Xpii
10.1007/s00203-016-1230-8journal_volume
198pub_type
杂志文章abstract：:Thermotoga hypogea is an extremely thermophilic anaerobic bacterium capable of growing at 90 degrees C. It uses carbohydrates and peptides as carbon and energy sources to produce acetate, CO(2), H(2), L-alanine and ethanol as end products. Alcohol dehydrogenase activity was found to be present in the soluble fraction ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0217-x
更新日期：2007-06-01 00:00:00
abstract：:In autotrophic methanogens, pyruvate oxidoreductase (POR) plays a key role in the assimilation of CO(2) and the biosynthesis of organic carbon. This enzyme has been purified to homogeneity, and the genes from Methanococcus maripaludis were sequenced. The purified POR contained five polypeptides with molecular masses o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0554-3
更新日期：2003-06-01 00:00:00
abstract：:The thermophilic bacterium Thermoanaerobacter tengcongensis has two single-stranded DNA-binding (SSB) proteins, designated TteSSB2 and TteSSB3. In a SSB complementation assay in Escherichia coli, only TteSSB3 took over the in vivo function of EcoSSB. We have cloned the ssb genes obtained by PCR and have developed E. c...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0366-6
更新日期：2008-07-01 00:00:00
abstract：:Pandoraea sp. strain MCB032 was isolated as an emerging chlorobenzene degrader from a functionally stable bioreactor where species succession had occurred. In this study, two gene clusters encoding chlorobenzene metabolic functions have been cloned. Within the cbs gene cluster, CbsA and CbsB are similar to the chlorob...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0476-9
更新日期：2009-06-01 00:00:00
abstract：:Among proteins specifically found in most gram-positive bacteria of the phylum Firmicutes, conserved proteins of the family pfam06177-DUF988-COG4708 are of unknown function. The citrulline cluster-linked (ccl) gene of Lactobacillus plantarum codes one such protein and is adjacent to the citrulline biosynthesis operon ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0774-9
更新日期：2005-08-01 00:00:00
abstract：:Bacillus thuringiensis (Bt) is efficient, strongly specific, and avirulent to humans, making it one of the most popular biopesticides in the world. Bt LLP29 is a mosquitocidal strain that was first isolated from Magnolia denudata. To understand its molecular mechanism against mosquitoes, the genome of Bt LLP29 was seq...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01875-2
更新日期：2020-09-01 00:00:00
abstract：:Bacteriochlorophyll (BChl) c in whole cells of Chlorobium tepidum grown at 46 degrees C changed into bacteriopheophytin (BPhe) c within 10 days after reaching full growth. When a small amount of C. tepidum cells in which BChl c had been completely pheophytinized were transferred to a new culture medium, normal growth ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050737
更新日期：1999-07-01 00:00:00
abstract：:The main aim of our study was to determine the physiological function of NagA enzyme in the Listeria monocytogenes cell. The primary structure of the murein of L. monocytogenes is very similar to that of Escherichia coli, the main differences being amidation of diaminopimelic acid and partial de-N-acetylation of gluco...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0752-3
更新日期：2012-04-01 00:00:00
abstract：:Bacteria in Lactobacillus casei group, including Lactobacillus casei (L. casei), Lactobacillus paracasei (L. paracasei), and Lactobacillus rhamnosus (L. rhamnosus) are important lactic acid bacteria in the production of fermented dairy products and are faced with the controversial nomenclatural status due to their clo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-018-1528-9
更新日期：2018-09-01 00:00:00
abstract：:Yeast suffers from a variety of environmental stresses, such as osmotic pressure and ethanol produced during fermentation. Since calcium ions are protective for high concentrations of ethanol, we investigated whether Ca(2+) flux occurs in response to ethanol stress. We find that exposure of yeast to ethanol induces a ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0673-6
更新日期：2011-05-01 00:00:00
abstract：:A neutral protease (npr) (designated Bae16) toxic to nematodes was purified to homogeneity from the strain Bacillus nematocida. The purified protease showed a molecular mass of approximately 40 kDa and displayed optimal activity at 55 degrees C, pH 6.5. Bioassay experiments demonstrated that this purified protease cou...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0112-x
更新日期：2006-06-01 00:00:00
abstract：:The glutamate catabolism of three thermophilic syntrophic anaerobes was compared based on the combined use of [(13)C] glutamate NMR measurements and enzyme activity determinations. In some cases the uptake of intermediates from different pathways was studied. The three organisms, Caloramator coolhaasii, Thermanaerovib...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100286
更新日期：2001-07-01 00:00:00
abstract：:Synechococcus elongatus strain PCC7942 cells were grown in high or low environmental concentrations of inorganic C (high-C(i), low-C(i)) and subjected to a light shift from 50 micromol m(-2) s(-1) to 500 micromol m(-2) s(-1). We quantified photosynthetic reductant (O(2) evolution) and molar cellular contents of phycob...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0761-1
更新日期：2005-03-01 00:00:00
abstract：:Ostracods collected from shallow coral reefs in the Bahamas were found to exhibit blue light-stimulated orange fluorescence at night. Fluorescent spectra revealed the presence of orange fluorescence with a maximum emission at ~595 nm on the carapace of these ostracods, while scanning electron microscopy revealed a mor...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0911-9
更新日期：2013-09-01 00:00:00
abstract：:The Gram-positive bacterium Clavibacter michiganensis subsp. sepedonicus is the causal agent of bacterial wilt and ring rot of potato. So far, only two proteins have been shown to be essential for virulence, namely a plasmid-encoded cellulase CelA and a hypersensitive response-inducing protein. We have examined the re...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0301-2
更新日期：2008-02-01 00:00:00
abstract：:A Gram-stain-negative, strictly aerobic, beige-pigmented, motile, chemoheterotrophic, rod-shaped or ovoid bacterium, designated strain KMU-37T, was isolated from seawater at Najeong Beach in the Republic of Korea. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1329-y
更新日期：2017-05-01 00:00:00
abstract：:Lipase is an important commercial enzyme with unique and versatile biotechnological applications. This study was conducted to biosynthesize and characterizes alkaliphilic lipase by Exiguobacterium sp. strain AMBL-20T isolated from the glacial water samples of the northeastern (Gilgit-Baltistan) region of Pakistan. The...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02133-1
更新日期：2020-12-15 00:00:00
abstract：:Edwardsiella tarda (E. tarda) is distributed widely in a variety of hosts including humans, other mammals and fish, and it is worthwhile to notice that E. tarda -caused fish infections lead to the most important bacterial disease in fish. Considering Eha acting as a transcriptional regulator in E. tarda strain ET13 ha...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01794-x
更新日期：2020-05-01 00:00:00
abstract：:Regulation of transcription of the Escherichia coli moe operon, which codes for proteins connecting molybdate metabolism, molybdopterin synthesis, and apomolybdoenzyme synthesis, was investigated. Expression of the moe operon was independent of genes coding for molybdate transport and Mo-cofactor biosynthesis. Express...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100252
更新日期：2001-03-01 00:00:00
abstract：:CO2 is required continuously during germination of Streptomyces viridochromogenes spores. Spores incubated in a defined germination medium in the absence of CO2 remain phase bright and do not release spore carbon. In the presence of CO2, the spores initiate germination accompanied by loss of refractility and spore car...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429118
更新日期：1978-09-01 00:00:00
abstract：:The clones generated in a sequencing project represent a resource for subsequent analysis of the organism whose genome has been sequenced. We describe an interrelated group of cloning vectors that either integrate into the genome or replicate, and that enhance the utility, for developmental and other studies, of the c...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0276-z
更新日期：2007-12-01 00:00:00
abstract：:A temperature-sensitive mutant of Salmonella typhimurium was isolated earlier after transposon mutagenesis with Tn10d Tet. The mutant D220 grows well at 28 degreesC but has a lower growth rate and forms filaments at 37 degreesC. Transposon-flanking fragments of mutant D220 DNA were cloned and sequenced. The transposon...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050607
更新日期：1998-06-01 00:00:00
abstract：:The activities of NAD+-photoreduction and NADH/decyl-ubiquinone reductase in membrane preparations of Rhodobacter capsulatus changed to the same extent under different conditions. These results indicated that NADH:ubiquinone oxidoreductase (complex I) catalyzes the electron transport in the downhill direction (respira...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050548
更新日期：1998-02-01 00:00:00
abstract：:The effects of the Ca(2+)/H(+) exchanger A23187 and the K(+)/H(+) exchanger nigericin, the electrogenic membrane-potential depleters valinomycin and CCCP, and the calcium channel blockers ruthenium red, nifedipine, and nitrendipine on the apical growth of Phycomyces blakesleeanus were analyzed. While all of the compou...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0609-5
更新日期：2003-12-01 00:00:00
abstract：:To identify virulence-associated genes of a fish pathogen Yersinia ruckeri, we screened a total of 1056 mini-Tn5-Km2 signature-tagged mutants in rainbow trout by immersion challenge. Of 1056, 25 mutants were found survival-defective as they could not be re-isolated from fish kidney 7 days after infection. Mutated gene...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0582-8
更新日期：2010-07-01 00:00:00
abstract：:Carotenogenic mutants of Corynebacterium glutamicum were analyzed for their carotenoid content. Mutant MV10 accumulated the same carotenoids as the wild-type, decaprenoxanthin, decaprenoxanthin monoglucoside, and (2R,6R,2'R,6'R)-decaprenoxanthin di-(beta-D)-glucoside, but in three-fold higher amounts. In addition, dec...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100315
更新日期：2001-09-01 00:00:00
abstract：:Bacteroides succinogenes produces acetate and succinate as major products of carbohydrate fermentation. An investigation of the enzymes involved indicated that pyruvate is oxidized by a flavin-dependent pyruvate cleavage enzyme to acetyl-CoA and CO2. Active CO2 exchange is associated with the pyruvate oxidation system...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00402302
更新日期：1978-05-30 00:00:00
abstract：:The effect of intestinal molecules produced by the host on the virulence of Pseudomonas fluorescens is poorly documented. In the present work, we evaluated the secretion of human β-defensin-2 (hBD-2) by enterocytes after infection with P. fluorescens (a species previously suggested to be involved in inflammatory bowel...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0865-3
更新日期：2013-03-01 00:00:00
abstract：:Elevation gradients, often regarded as "natural experiments or laboratories", can be used to study changes in the distribution of microbial diversity related to changes in environmental conditions that typically occur over small geographical scales. We exploited this feature by characterizing fungal composition and di...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01948-2
更新日期：2020-10-01 00:00:00
abstract：:Of the seven amino acids required by Clostridium botulinum type E, tryptophan is the most essential and may provide the cell with nitrogen. The addition of excess tryptophan (10-20 mM) or other nitrogenous nutrients to minimal growth medium markedly decreased toxin formation but did not affect growth in C. botulinum t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245225
更新日期：1990-01-01 00:00:00
