Abstract：
:Xanthan-deficient mutants of Xanthomonas axonopodis pv. citri, the bacterium responsible for citrus canker, were generated by deletion and marker exchange of the region encoding the carboxy-terminal end of the first glycosyltransferase, GumD. Mutants of gumD did not produce xanthan and remained pathogenic in citrus plants to the same extent as wild-type bacteria. The kinetics of appearance of initial symptoms, areas of plant material affected, and growth of bacteria inside plant tissue throughout the disease process were similar for both wild-type and mutant inoculations. Moreover, exopolysaccharide deficiency did not impair the ability of the bacteria to induce hypersensitive response on non-host plants. Apart from variations in phenotypic aspects, no differences in growth or survival under different stress conditions were observed between the xanthan-deficient mutant and wild-type bacteria. However, gumD mutants displayed impaired survival under oxidative stress during stationary phase as well as impaired epiphytic survival on citrus leaves. Our results suggest that xanthan does not play an essential role in citrus canker at the initial stages of infection or in the incompatible interactions between X. axonopodis pv. citri and non-host plants, but facilitates the maintenance of bacteria on the host plant, possibly improving the efficiency of colonization of distant tissue.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Dunger G,Relling VM,Tondo ML,Barreras M,Ielpi L,Orellano EG,Ottado Jdoi
10.1007/s00203-007-0227-8subject
Has Abstractpub_date
2007-08-01 00:00:00pages
127-35issue
2eissn
0302-8933issn
1432-072Xjournal_volume
188pub_type
杂志文章abstract：:1. Culture filtrates of heterotrophic bacteria were tested for their stimulatory effect on nitrification of three strains of Nitrobacter. 2. Yeast extract-peptone solution, in which Pseudomonas fluorescens had grown, after removal of the cells was added to autotrophically growing cultures of Nitrobacter agilis; it cau...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454856
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1104-5
更新日期：2015-06-01 00:00:00
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journal_title：Archives of microbiology
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1977-09-28 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1979-03-12 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-0975-1
更新日期：2014-06-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
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更新日期：2001-04-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1977-05-13 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1977-02-04 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：2000-07-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446644
更新日期：1976-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2012-09-01 00:00:00
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更新日期：2010-08-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-1069-9
更新日期：2015-04-01 00:00:00
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pub_type： 杂志文章
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447300
更新日期：1975-06-20 00:00:00
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更新日期：2002-04-01 00:00:00
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更新日期：2014-04-01 00:00:00
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更新日期：2020-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2013-03-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1997-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：1992-01-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2020-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2004-02-01 00:00:00
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journal_title：Archives of microbiology
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更新日期：2013-06-01 00:00:00
