Abstract：
:To clarify the deactivation mechanism of pyruvate formate-lyase (PFL) and its role in the regulation of fermentation in Streptococcus bovis, the molecular properties and genetic expression of multifunctional alcohol dehydrogenase (ADHE) were investigated. S. bovis was found to have ADHE, which was deduced to consist of 872 amino acids with a molecular mass of 97.4 kDa. The ADHE was shown to harbor three enzyme activities: (1) alcohol dehydrogenase, (2) coenzyme-A-linked acetaldehyde dehydrogenase that catalyzes the conversion of acetyl-CoA to ethanol, and (3) PFL deactivase. Similar to Escherichia coli ADHE, S. bovis ADHE required Fe2+ for its activity. The gene encoding ADHE ( adhE) was shown to be monocistronic. The level of adhE mRNA changed in parallel with the mRNA levels of the genes encoding PFL (pfl) and PFL-activating enzyme (act) as the growth conditions changed, although these genes are independently transcribed. Synthesis of ADHE, PFL-activating enzyme, and PFL appears to be regulated concomitantly. Overexpression of ADHE did not cause a change in the formate-to-lactate ratio. It is conceivable that ADHE is not significantly involved in the reversible inactivation of active PFL under anoxic conditions. Partition of the flow from pyruvate appears to be mainly regulated by the activities of lactate dehydrogenase and PFL.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Asanuma N,Yoshii T,Hino Tdoi
10.1007/s00203-003-0638-0keywords:
subject
Has Abstractpub_date
2004-02-01 00:00:00pages
122-8issue
2eissn
0302-8933issn
1432-072Xjournal_volume
181pub_type
杂志文章abstract：:Two styrene monooxygenase types, StyA/StyB and StyA1/StyA2B, have been described each consisting of an epoxidase and a reductase. A gene fusion which led to the chimeric reductase StyA2B and the occurrence in different phyla are major differences. Identification of SMOA/SMOB-ADP1 of Acinetobacter baylyi ADP1 may enlig...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-1022-y
更新日期：2014-12-01 00:00:00
abstract：:Bacteriochlorophyll (BChl) c in whole cells of Chlorobium tepidum grown at 46 degrees C changed into bacteriopheophytin (BPhe) c within 10 days after reaching full growth. When a small amount of C. tepidum cells in which BChl c had been completely pheophytinized were transferred to a new culture medium, normal growth ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050737
更新日期：1999-07-01 00:00:00
abstract：:Triacylglycerol granules synthesized and accumulated by Rhodococcus opacus and Rhodococcus ruber were isolated by glycerol density gradient centrifugation. Whereas only one type of granule could be isolated from R. opacus, two types of granules with different specific densities were isolated from R. ruber. Both types ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-001-0355-5
更新日期：2001-12-01 00:00:00
abstract：:Acinetobacter calcoaceticus LMD 79.41 produced significant amounts of pyrrolo-quinoline quinone (PQQ) in its culture medium when grown on quinic acid or shikimic acid. Studies with LMD 79.41 and PQQ- -mutants of this strain demonstrated that this organism contains an NAD(P)-independent quinate dehydrogenase (QDH) (EC ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00409714
更新日期：1988-05-01 00:00:00
abstract：:The thermotolerant methylotroph Bacillus sp. C1 possesses a novel NAD-dependent methanol dehydrogenase (MDH), with distinct structural and mechanistic properties. During growth on methanol and ethanol, MDH was responsible for the oxidation of both these substrates. MDH activity in cells grown on methanol or glucose wa...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245161
更新日期：1992-01-01 00:00:00
abstract：:Calcium is essential for the growth of rhizobia and the formation of nitrogen-fixing root-nodules on legumes, but its precise role in these processes remains unknown. We have found that Rhizobium fredii USDA208 accumulates a major 38 kDa protein when grown in media supplemented with 0.3-2 microM Cacl2. We have purifie...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00248480
更新日期：1993-01-01 00:00:00
abstract：:Fructose transport was not apparently affected in a number of Pseudomonas putida strains with deranged activity of a common glucose-gluconate uptake system, indicating the existence of an independent fructose uptake system. Fructose uptake by glucose-gluconate uptake mutants was induced by fructose and obeyed saturati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428362
更新日期：1975-01-01 00:00:00
abstract：:We describe a convenient method for the in vivo construction of large plasmids that possess a multitude of restriction sites. A large (23 kbases) circular self-replicating plasmid carrying a partial LEU2-d gene was cotransformed with a circular non-replicating plasmid carrying the entire LEU2 gene. In vivo recombinati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00409661
更新日期：1989-01-01 00:00:00
abstract：:Recently, we reported the synthesis of five bacteriocin-like inhibitor substances (Bt-BLIS: morricin 269, kurstacin 287, kenyacin 404, entomocin 420, and tolworthcin 524) by Mexican strains of Bacillus thuringiensis. Here we show that, collectively, these Bt-BLIS have a moderate to broad spectrum of antibacterial acti...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0414-2
更新日期：2008-12-01 00:00:00
abstract：:Mercury resistance of Clostridium cochlearium T-2P was found to be controlled by a different mechanism from those reported so far since no mercury-reducing activity was detected in this strain. The H2S generating ability as well as the demethylating activity in this bacterium was eliminated by the treatment of the cur...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00417179
更新日期：1981-03-01 00:00:00
abstract：:A 2.3-L bioreactor was specially adapted to grow hyperthermophilic microorganisms under controlled conditions of temperature, pH, redox potential and dissolved O(2). Using this bioreactor regulated at 80°C and pH 7.0, we demonstrated that Thermotoga maritima recovered its growth despite being exposed to oxygen for a s...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0687-8
更新日期：2011-06-01 00:00:00
abstract：:Bioleaching is a promising process for 350 million tons Jinchuan low-grade pentlandite. But, Jinchuan pentlandite has lots of magnesium and high concentration of Mg2+ is harmful to bioleaching microorganisms. Thus, finding a way to improve the adaption of microorganisms to Mg2+ is a key for bioleaching. In the study, ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-018-1503-5
更新日期：2018-09-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0533-4
更新日期：2010-02-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01621-3
更新日期：2019-04-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00402336
更新日期：1986-11-01 00:00:00
abstract：:The presence of one periplasmic [NiFe] hydrogenase, one periplasmic [Fe] hydrogenase, and one cytoplasmic NADP-reducing hydrogenase has been previously established in Desulfovibrio fructosovorans. In the present work, marker-exchange mutagenesis was performed to determine the function of the tetrameric NADP-reducing h...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050414
更新日期：1997-01-01 00:00:00
abstract：:Listeria monocytogenes was examined for the presence of surface carbohydrates to ascertain whether surface sugars, if present, would interact with eucaryotic surface carbohydrate receptors. We found that a virulent, but not two avirulent strains had a surface alpha-D-galactose residue as determined by agglutination wi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00249083
更新日期：1990-01-01 00:00:00
abstract：:The subtype of phosphofructokinase activity, either ATP-, ADP- or pyrophosphate-dependent, present in members of three genera from the Spirochaetales was investigated. The individual species/strains examined included Spirochaeta alkalica, S. asiatica, S. halophila, S. isovalerica, S. litoralis, S. zuelzerae, S. thermo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050777
更新日期：1999-12-01 00:00:00
abstract：:The fine structure of the Gram-negative filamentous gliding bacterium, Herpetosiphon is described. The outer membrane of the cell envelope could not be resolved as a separate structure, probably because it is fused with the underlying dense (peptidoglycan) layer. There was an additional wall layer outside this membran...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428379
更新日期：1975-03-10 00:00:00
abstract：:Sporothrix schenckii is the etiological agent of sporotrichosis, a mycosis of humans and other mammals. Little is known about the responses of this thermodimorphic pathogen to perturbations in the cell wall (CW) by different stress conditions. Here we describe the effect of Congo Red (CR) on the fungal growth, morphog...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-018-1576-1
更新日期：2019-01-01 00:00:00
abstract：:The fast-growing Rhizobium sp. strain NGR234, isolated from Papua New Guinea, and 13 strains of Sinorhizobium fredii, isolated from China and Vietnam, were fingerprinted by means of RAPD, REP, ERIC and ARDRA. ERIC, REP and RAPD markers revealed a considerable genetic diversity among fast-growing rhizobia. Chinese isol...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0559-y
更新日期：2003-07-01 00:00:00
abstract：:We investigated the structural and immunochemical characteristics of cell wall mannan obtained from Candida sojae JCM 1644, which is a new yeast species isolated from defatted soybean flakes. The results of a slide-agglutination test and of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0339-1
更新日期：2008-05-01 00:00:00
abstract：:Catalytic properties and membrane associations of the phosphatidylglycerophosphate (PGP) and phosphatidylserine (PS) synthases of Rhodobacter sphaeroides were examined to further characterize sites of phospholipid biosynthesis. In preparations of cytoplasmic membrane (CM) enriched in these activities, apparent Km valu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00456090
更新日期：1989-01-01 00:00:00
abstract：:The L-alanine mediated germination of food isolated Bacillus cereus DSA 1 spores, which lacked an intact exosporium, increased in the presence of D-cycloserine (DCS), which is an alanine racemase (Alr) inhibitor, reflecting the activity of the Alr enzyme, capable of converting L-alanine to the germination inhibitor D-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0946-y
更新日期：2014-02-01 00:00:00
abstract：:Monoclonal antibodies have been used to determine the presence of cellobiohydrolases I and II (CBH I and II), and endoglucanase I (EG I) on the surface of conidia from Trichoderma reesei QM 9414 and RUT C-30, and 8 other Trichoderma species. For this purpose, proteins were released from the conidial surface by treatme...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245356
更新日期：1991-01-01 00:00:00
abstract：:Experiments were performed to isolate mutants lacking alkaline phosphatase in Chlamydomonas reinhardi. Mutants with null enzyme activity were obtained. A cytological study of these mutants however revealed cell wall defects, suggesting that the loss of phosphatase activity in these strains is not due to the inactivati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00436357
更新日期：1975-05-05 00:00:00
abstract：:The induction by D,L-nicotine of the enantiozymes 6-hydroxy-L-nicotine oxidase and 6-hydroxy-D-nicotine oxidase in Arthrobacter oxidans was differently affected by the inhibitors of Escherichia coli gyrase, novobiocin and nalidixic acid. These compounds inhibited 6-hydroxy-L-nicotine oxidase induction slightly, but le...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00521289
更新日期：1982-12-03 00:00:00
abstract：:Pseudomonas cepacia grew rapidly using a mixture of all three branched chain amino acids as carbon source, but failed to use individual branched chain amino acids as sole carbon source. Extracts of bacteria grown on branched chain amino acids had between 2- and 3-fold higher levels of alpha-ketoglutarate-dependent bra...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00423069
更新日期：1979-03-12 00:00:00
abstract：:The molecular weight of the protein moiety of a phospholipoprotein complex isolated from Pseudomonas facilis has been examined with a variety of sodium dodecylsulfate-polyacrylamide gel electrophoretic systems. A molecular weight of 35 000 was determined for the protein in all analyses. A 35 000-dalton protein was pre...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00427842
更新日期：1977-10-24 00:00:00
abstract：:The evolutionarily conserved serine/threonine kinase TOR recruits different subunits to assemble the Target of Rapamycin Complex 1 (TORC1), which is inhibited by rapamycin and regulates ribosome biogenesis, autophagy, and lipid metabolism by regulating the expression of lipogenic genes. In addition, TORC1 participates...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01833-y
更新日期：2020-07-01 00:00:00
