Structural analysis of cell wall mannan of Candida sojae, a new yeast species isolated from defatted soybean flakes.

Abstract:

:We investigated the structural and immunochemical characteristics of cell wall mannan obtained from Candida sojae JCM 1644, which is a new yeast species isolated from defatted soybean flakes. The results of a slide-agglutination test and of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic Candida species indicated that the cells and the C. sojae mannan were cross-reactive to the specific anti-factor sera against Candida albicans serotype A (FAb 6) and Candida guilliermondii (FAb 9). Two-dimensional homonuclear Hartmann-Hahn analysis indicated that the mannan consisted of various linked oligomannosyl side chains containing alpha-1,2-, alpha-1,3-, alpha-1,6- and beta-1,2-linked mannose residues. However, although the determinants of antigenic factors 6 and 9 could be not found in this mannan, branched side chains, Manbeta1-2Manalpha1-3[Manalpha1-6]Manalpha1-(2Manalpha1-)n2Man and a linear alpha-1,6-linked polymannosyl backbone, which are cross-reacted by FAbs 6 and 9, respectively, were identified. The mannan was subjected to acetolysis in order to determine the polymerization length of the alpha-1,2-linked oligomannosyl residue in the side chains. The result of (1)H-nuclear magnetic resonance analysis of the released oligosaccharides showed that the remarkable regularity in the length of alpha-1,2-linked oligomannosyl side chains, which were previously found in mannans of other Candida species, is not observed in this mannan.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Oyamada H,Ogawa Y,Shibata N,Okawa Y,Suzuki S,Kobayashi H

doi

10.1007/s00203-007-0339-1

subject

Has Abstract

pub_date

2008-05-01 00:00:00

pages

483-90

issue

5

eissn

0302-8933

issn

1432-072X

journal_volume

189

pub_type

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