Abstract：
:Triacylglycerol granules synthesized and accumulated by Rhodococcus opacus and Rhodococcus ruber were isolated by glycerol density gradient centrifugation. Whereas only one type of granule could be isolated from R. opacus, two types of granules with different specific densities were isolated from R. ruber. Both types of R. ruber granules showed a similar content of triacylglycerols and poly(3-hydroxybutyrate- co-3-hydroxyvalerate), but the protein profiles of both types were significantly different. The granules with the lower specific density were colorless; the granules with the higher specific density had a deep orange pigmentation. Solubilization studies revealed three different groups of granule-associated proteins: (1) unspecifically bound proteins, (2) relatively weakly associated proteins, and (3) proteins that resisted solubilization by treatment with 2 M NaCl, 2% (w/v) Triton X-114, 6 M guanidinium hydrochloride, up to 8% (w/v) SDS, and proteolytic digestion. The strong association of proteins of the last group suggested that these may play a specific role in the synthesis or mobilization of storage lipids or in the structure of the granules. The N-terminal amino acid sequences of the most tightly bound proteins were obtained. Proteins of low molecular weight with striking sequence similarity to the ribosomal protein L7 from various actinomycetes were always copurified with the granules.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Kalscheuer R,Wältermann M,Alvarez M,Steinbüchel Adoi
10.1007/s00203-001-0355-5keywords:
subject
Has Abstractpub_date
2001-12-01 00:00:00pages
20-8issue
1eissn
0302-8933issn
1432-072Xjournal_volume
177pub_type
杂志文章abstract：:During cultivation of a gastric fungus, Coniochaeta polymorpha, growth of Nocardia colonies on top of the fungal culture raised the question whether bacteria originated from inside of fungus. In this study, the likelihood of intracellular origin of bacteria as well as interaction of two microorganisms was assessed. Fl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02122-4
更新日期：2021-01-02 00:00:00
abstract：:The site of integration of phage M mu d (Ap lac) in mutant M9s which leads to deficiency of formic dehydrogenase (benzylviologen-linked) activity was determined. It was shown that the phage had inserted into the gene for the seleno-polypeptide of the enzyme (80 kd) leading to the formation of a truncated peptide (60 k...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428850
更新日期：1985-05-01 00:00:00
abstract：:As the protein databases continue to expand at an exponential rate, fed by daily uploads from multiple large scale genomic and metagenomic projects, the problem of assigning a function to each new protein has become the focus of significant research interest in recent times. Herein, we review the most recent advances ...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-010-0549-9
更新日期：2010-03-01 00:00:00
abstract：:Mutants resistance to various combinations of threonine, lysine and/or their analogs were obtained and characterized in Pseudomonas acidovorans. In particular, mutants resistant to aminoethylcysteine had a dihydrodipicolinate synthetase insensitive to lysine inhibition whereas mutants resistant to threonine plus a low...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446845
更新日期：1976-03-19 00:00:00
abstract：:Helcococcus kunzii is a gram-positive, catalase-negative opportunist. The organism has been isolated from the lower extremities and breast masses of several patients. A clinical isolate of Helcococcus kunzii was shown to possess a hemagglutinin-lectin with a specificity for N-acetylglucosamine and lactose, two structu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-001-0378-y
更新日期：2002-02-01 00:00:00
abstract：:The presence of teichoic acids in a number of streptomycetes led to the conclusion that these biopolymers were widely spread in genus Streptomyces. The nature of the teichoic acid present in the mycelium was determined by extracting it with 10% trichloroacetic acid, precipitating it with ethanol and identifying the pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00421893
更新日期：1980-05-01 00:00:00
abstract：:Enzymes that are regulated by the ferredoxin/thioredoxin system in chloroplasts--fructose-1,6-bis-phosphatase (FBPase), sedoheptulose-1,7-bisphosphatase (SBPase), and phosphoribulokinase (PRK)--were partially purified from two different types of photosynthetic prokaryotes (cyanobacteria, purple sulfur bacteria) and te...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00401986
更新日期：1984-10-01 00:00:00
abstract：:The Streptomyces virginiae gamma-butyrolactone autoregulator virginiae butanolide is a low-molecular-weight Streptomyces hormone eliciting virginiamycin biosynthesis through its binding to the specific receptor protein, BarA. Immediately downstream of barA lies barB, the transcription of which is tightly repressed by ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0625-5
更新日期：2004-01-01 00:00:00
abstract：:Flow cytometry (FCM) in conjunction with immunocytochemical-labeling was used to analyze and screen a population of Escherichia coli clones containing a genomic library from the oil-degrading microorganism Acinetobacter calcoaceticus RAG-1 surface antigens. Reconstruction experiments using mixed populations indicated ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00422282
更新日期：1988-01-01 00:00:00
abstract：:With primers designed for the conserved region of the gamma-butyrolactone autoregulator receptor proteins from Streptomyces species, PCR using the Streptomyces clavuligerus genome DNA as a template gave a clear band of 100 bp, the sequence of which revealed high similarity to the expected region of a receptor gene. By...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0697-x
更新日期：2004-09-01 00:00:00
abstract：:Two intestinal bacterial strains MT4s-5 and MT42 involved in the degradation of (-)-epigallocatechin (EGC) were isolated from rat feces. Strain MT4s-5 was tentatively identified as Adlercreutzia equolifaciens. This strain converted EGC into not only 1-(3, 4, 5-trihydroxyphenyl)-3-(2, 4, 6-trihydroxyphenyl)propan-2-ol ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-1006-y
更新日期：2014-10-01 00:00:00
abstract：:Milk containing antibiotics is used as cost-effective feed for calves, which may lead to antibiotic residues-associated food safety problems. This study aims to investigate the influence of antibiotics on rumen microbes. Through metagenomic sequencing, the rumen microbial communities of calves fed with pasteurized mil...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1311-8
更新日期：2017-04-01 00:00:00
abstract：:We describe a convenient method for the in vivo construction of large plasmids that possess a multitude of restriction sites. A large (23 kbases) circular self-replicating plasmid carrying a partial LEU2-d gene was cotransformed with a circular non-replicating plasmid carrying the entire LEU2 gene. In vivo recombinati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00409661
更新日期：1989-01-01 00:00:00
abstract：:Bioleaching is a promising process for 350 million tons Jinchuan low-grade pentlandite. But, Jinchuan pentlandite has lots of magnesium and high concentration of Mg2+ is harmful to bioleaching microorganisms. Thus, finding a way to improve the adaption of microorganisms to Mg2+ is a key for bioleaching. In the study, ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-018-1503-5
更新日期：2018-09-01 00:00:00
abstract：:Autotrophic cultures of the facultative chemolithotroph Alcaligenes eutrophus have been found to excrete glycollate. This excretion was greatly stimulated by the incorporation of up to 20% (v/v) oxygen in the hydrogen used for gassing. The stimulatory effect of oxygen was prevented by the addition of 10% (v/v) CO2 to ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00690224
更新日期：1976-11-02 00:00:00
abstract：:Photosynthetic, nitrogen-fixing Anabaena strains play an important role in the carbon and nitrogen cycles in tropical paddy fields although they are salt sensitive. Improvement in salt tolerance of Anabaena cells by expressing glycine betaine-synthesizing genes is an interesting subject. Due to the absence of choline ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0824-z
更新日期：2012-11-01 00:00:00
abstract：:The biological effects of UV radiation of different wavelengths (UVA, UVB and UVC) were assessed in nine bacterial isolates displaying different UV sensitivities. Biological effects (survival and activity) and molecular markers of oxidative stress [DNA strand breakage (DSB), generation of reactive oxygen species (ROS)...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0847-5
更新日期：2013-01-01 00:00:00
abstract：:An aerobic, Gram-negative, motile, rod-shaped bacterium designated KMM 9500(T) was isolated from a sediment sample collected from the Sea of Japan seashore. Comparative 16S rRNA gene sequence analysis affiliated strain KMM 9500(T) to the genus Pseudomonas as a distinct subline clustered with Pseudomonas marincola KMM ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1103-6
更新日期：2015-06-01 00:00:00
abstract：:Carotenogenic mutants of Corynebacterium glutamicum were analyzed for their carotenoid content. Mutant MV10 accumulated the same carotenoids as the wild-type, decaprenoxanthin, decaprenoxanthin monoglucoside, and (2R,6R,2'R,6'R)-decaprenoxanthin di-(beta-D)-glucoside, but in three-fold higher amounts. In addition, dec...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100315
更新日期：2001-09-01 00:00:00
abstract：:L-Alanine dehydrogenase was found in extracts of the antibiotic producer Streptomyces clavuligerus. The enzyme was induced by ammonia, and the level of induction was dependent on the extracellular concentraction. L-Alanine was the only amino acid able to induce alanine dehydrogenase. The enzyme was characterized from ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00403210
更新日期：1980-03-01 00:00:00
abstract：:Experiments were performed to isolate mutants lacking alkaline phosphatase in Chlamydomonas reinhardi. Mutants with null enzyme activity were obtained. A cytological study of these mutants however revealed cell wall defects, suggesting that the loss of phosphatase activity in these strains is not due to the inactivati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00436357
更新日期：1975-05-05 00:00:00
abstract：:We previously constructed an arabinose-inducible recombinant Bifidobacterium longum that could efficiently express secreted IFN-alpha2b in vitro (Deng et al. in Arch Microbiol 191:681-686, 2009). Here, we investigated the influence of oral pBAD-SPIFN-transformed B. longum on immunomodulation and IFN-induced Mx1 gene t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0589-1
更新日期：2010-08-01 00:00:00
abstract：:Microbial changes in vaginal ecosystem may accelerate the process of cervical carcinogenesis. The developed cervical cancer can lead to changes in the vaginal microbiota. The aim of our study is to determine the vaginal microbiota changes at women with FIGO I stage cervical cancer. We conducted an open, single-site su...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01747-4
更新日期：2020-03-01 00:00:00
abstract：:Mycothiol is the predominant thiol in most actinomycetes, including Mycobacterium tuberculosis, and appears to play a role analogous to glutathione, which is not found in these bacteria. The enzymes involved in mycothiol biosynthesis are of interest as potential targets for new drugs directed against tuberculosis. In ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0462-y
更新日期：2002-11-01 00:00:00
abstract：:The synthesis of the luminous system of the marine luminous bacterium Photobacterium fischeri is subject to a complex, self-regulated control system called autoinduction. The bacteria produce an autoinducer which accumulates in the medium at a constant rate (as a function of cell growth). When autoinducer reaches a cr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446657
更新日期：1977-02-04 00:00:00
abstract：:Pulicat Lake sediments are often severely polluted with the toxic heavy metal mercury. Several mercury-resistant strains of Bacillus species were isolated from the sediments and all the isolates exhibited broad spectrum resistance (resistance to both organic and inorganic mercuric compounds). Plasmid curing assay show...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0088-6
更新日期：2006-04-01 00:00:00
abstract：:The metabolism of methylamine as the nitrogen source for growth of the non-methylotrophic yeast Candida utilis and the methylotrophic yeast Hansenula polymorpha was investigated. Growth of both organisms in media with glucose and methylamine was associated with the presence of an amine oxidase in these cells. The enzy...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00511216
更新日期：1980-06-01 00:00:00
abstract：:Mercury resistance of Clostridium cochlearium T-2P was found to be controlled by a different mechanism from those reported so far since no mercury-reducing activity was detected in this strain. The H2S generating ability as well as the demethylating activity in this bacterium was eliminated by the treatment of the cur...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00417179
更新日期：1981-03-01 00:00:00
abstract：:Two different acidic polysaccharides (I and II) were detected in the external cell envelope layers (slime and capsule) of Rhodopseudomonas capsulata Sp11. Polysaccharide I contains rhamnose, fucose, glucosamine and an unknown acidic sugar, it represents the slime material of the strain. Polysaccharide II contains rham...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425219
更新日期：1983-12-01 00:00:00
abstract：:A triazine dye, Cibacron blue 3G-A (CB), is an inhibitor of cell separation of staphylococcal spp. therefore, we examined the effect of CB on growth of gram-positive bacteria other than Staphylococcus. CB added to the medium of growing cultures of strains of genus Micrococcus, Streptococcus, Lactobacillus and Bacillus...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00248617
更新日期：1991-01-01 00:00:00
