Flow cytometric screening and isolation of Escherichia coli clones which express surface antigens of the oil-degrading microorganism Acinetobacter calcoaceticus RAG-1.

Abstract:

:Flow cytometry (FCM) in conjunction with immunocytochemical-labeling was used to analyze and screen a population of Escherichia coli clones containing a genomic library from the oil-degrading microorganism Acinetobacter calcoaceticus RAG-1 surface antigens. Reconstruction experiments using mixed populations indicated that RAG-1 cells could be clearly distinguished at a ratio of one RAG-1 cell to 500 Escherichia coli cells. Using this technique two clones, WM143 and WM191, were isolated and shown by restriction endonuclease cleavage and Southern hybridization to contain plasmids carrying inserts of RAG-1 DNA of 9.4 and 9.8 kb respectively.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Minas W,Sahar E,Gutnick D

doi

10.1007/BF00422282

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

432-7

issue

5

eissn

0302-8933

issn

1432-072X

journal_volume

150

pub_type

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