Abstract:
:Flow cytometry (FCM) in conjunction with immunocytochemical-labeling was used to analyze and screen a population of Escherichia coli clones containing a genomic library from the oil-degrading microorganism Acinetobacter calcoaceticus RAG-1 surface antigens. Reconstruction experiments using mixed populations indicated that RAG-1 cells could be clearly distinguished at a ratio of one RAG-1 cell to 500 Escherichia coli cells. Using this technique two clones, WM143 and WM191, were isolated and shown by restriction endonuclease cleavage and Southern hybridization to contain plasmids carrying inserts of RAG-1 DNA of 9.4 and 9.8 kb respectively.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Minas W,Sahar E,Gutnick Ddoi
10.1007/BF00422282subject
Has Abstractpub_date
1988-01-01 00:00:00pages
432-7issue
5eissn
0302-8933issn
1432-072Xjournal_volume
150pub_type
杂志文章abstract::The histidine protein kinase SppK is a peptide pheromone-activated kinase that regulates the production of the bacteriocin sakacin P in Lactobacillus sakei. SppK belongs to subfamily 10 of histidine protein kinases (HPKs), which regulate important processes in Gram-positive bacteria, including virulence, competence an...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0049-5
更新日期:2006-01-01 00:00:00
abstract::Anguibactin, an important virulent factor in Vibrio anguillarum serotype O1, is synthesized by a nonribosomal peptide synthetases (NRPS) system encoded on a 65-kb virulence plasmid pJM1. angE, as one of the NRPS genes, is responsible for selecting and activating 2,3-dihydroxybenzoic acid (2,3-DHBA), an important precu...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0652-x
更新日期:2004-04-01 00:00:00
abstract::A moderately psychrophilic purple non-sulfur bacterium, Rhodoferax antarcticus strain Fryx1, is described. Strain Fryx1 was isolated from the water column under the ice of the permanently frozen Lake Fryxell, Antarctica. Cells of Fryx1 are long thin rods and contain gas vesicles, the first report of such structures in...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0719-8
更新日期:2004-10-01 00:00:00
abstract::Mycothiol (MSH) plays important roles in maintaining cytosolic redox homeostasis and in adapting to reactive oxygen species in the high-(G + C)-content Gram-positive Actinobacteria. However, its physiological roles are ill defined compared to glutathione, the functional analog of MSH in Gram-negative bacteria and most...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-013-0889-3
更新日期:2013-06-01 00:00:00
abstract::A thermostable homodimeric isocitrate dehydrogenase from the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus was purified and characterized. The mol. mass of the isocitrate dehydrogenase subunit was 42 kDa as determined by SDS-PAGE. Following separation by SDS-PAGE, A. fulgidus isocitrate dehydrogen...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050516
更新日期:1997-11-01 00:00:00
abstract::A bacterium capable of using the carbamate insecticide carbofuran as a sole source of carbon and energy, was isolated from soil. The ability to catabolise carbofuran phenol, produced by cleavage of the carbamate ester linkage of the insecticide, was lost at very high frequency when the bacterium was grown in the absen...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00245249
更新日期:1992-01-01 00:00:00
abstract::Chlorobenzoates (CBA) arise as intermediates during the degradation of polychlorinated biphenyls (PCBs) and some chlorinated herbicides. Since PCBs were produced as complex mixtures, a range of mono-, di-, and possibly trichloro-substituted benzoates would be formed. Chlorobenzoate degradation has been proposed to be ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF01692864
更新日期:1996-03-01 00:00:00
abstract::Aflatoxins are highly toxic and carcinogenic fungal secondary metabolites. At least 18 enzyme activities are required for aflatoxin biosynthesis in the filamentous fungus Aspergillus parasiticus. One of these enzymes, versicolorin B synthase (VBS), catalyzes bisfuran ring closure in versiconal hemiacetal (a reaction n...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0700-6
更新日期:2004-09-01 00:00:00
abstract::The diversity among 45 cyanobacterial isolates from 11 different Gunnera species originating from different geographical areas was examined. By means of polymerase chain reaction (PCR) fingerprinting with short tandemly repeated repetitive (STRR) sequences as primers, ten groups of symbiotic cyanobacteria and five uni...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002039900113
更新日期:2000-02-01 00:00:00
abstract::An unique isotope labelling experiment was conducted whereby mixtures of sulfate and sulfite of different isotopic compositions were metabolized by Clostridium pasteurianum. The results showed during reduction of 1 mM SO3= plus 1 mM SO4=, essentially all evolved H2S arose from the sulfite whereas in the case of cellul...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446644
更新日期:1976-09-01 00:00:00
abstract::In a soil/sediment model system we have shown recently that a gram-positive bacterium with natural competence (Bacillus subtilis) can take up transforming DNA adsorbed to sand minerals. Here we examined whether also a naturally transformable soil bacterium of the gram-negative pseudomonad (Pseudomonas stutzeri) can be...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00276535
更新日期:1990-01-01 00:00:00
abstract::The Sinorhizobium fredii HH103 rkp-1 region, which is involved in capsular polysaccharide (KPS) biosynthesis, is constituted by the rkpU, rkpAGHIJ, and kpsF3 genes. Two mutants in this region affecting the rkpA (SVQ536) and rkpI (SVQ538) genes were constructed. Polyacrylamide gel electrophoresi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-011-0729-2
更新日期:2012-02-01 00:00:00
abstract::Effect of acidic condition on the fermentation characteristics was investigated by the continuous culture of Escherichia coli. In accordance with down-regulation of crp gene transcript level as well as up-regulation of arcA, the expressions of the TCA cycle genes were down-regulated, which caused more acetate formatio...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-012-0861-7
更新日期:2013-03-01 00:00:00
abstract::Short-chain aliphatic epoxides and ketones are two classes of toxic organic compounds formed biogenically and anthropogenically. In spite of their toxicity, these compounds are utilized as primary carbon and energy sources or are generated as intermediate metabolites in the metabolism of other compounds (e.g., alkenes...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s002030050558
更新日期:1998-03-01 00:00:00
abstract::A novel thermotolerant strain of the achlorophyllous micro-alga Prototheca was isolated from a hot spring. The isolate was found to produce an appreciable amount of ethanol and CO2 from glucose under anoxic conditions at both 25 and 40 degrees C; this type of alcohol fermentation has not yet been reported in the genus...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-001-0384-0
更新日期:2002-03-01 00:00:00
abstract::Citrobacter rodentium (Cr) is a mouse pathogen that mimics many aspects of enteropathogenic Escherichia coli infections including producing attaching and effacing (A/E) lesions. Host-adapted (HA) Cr cells that are shed at the peak of infection have been reported to be hyper-infective. The exact mechanism underlying th...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1191-y
更新日期:2016-05-01 00:00:00
abstract::A highly efficient method of transposon mutagenesis was developed for genetic analysis of Xanthobacter autotrophicus Py2. The method makes use of a transposon delivery vector that encodes a hyperactive Tn 5 transposase that is 1,000-fold more active than the wild-type transposase. In this construct, the transposase is...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0442-2
更新日期:2002-09-01 00:00:00
abstract::Gene probe hybridization was used to determine distribution and expression of co-metabolic genes at a contaminated site as it underwent in situ methanotrophic bioremediation of trichloroethylene (TCE). The bioremediation strategies tested included a series of air, air:methane, and air:methane:nutrient pulses of the te...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-008-0445-8
更新日期:2009-03-01 00:00:00
abstract::Monoclonal antibodies have been used to determine the presence of cellobiohydrolases I and II (CBH I and II), and endoglucanase I (EG I) on the surface of conidia from Trichoderma reesei QM 9414 and RUT C-30, and 8 other Trichoderma species. For this purpose, proteins were released from the conidial surface by treatme...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00245356
更新日期:1991-01-01 00:00:00
abstract::Escherichia coli, Salmonella enterica, Klebsiella pneumoniaeand Klebsiella oxytocawere found to contain two D-tagatose 1,6-bisphosphate (TagBP)-specific aldolases involved in catabolism of galactitol (genes gatY gatZ) and of N-acetyl-galactosamine and D-galactosamine (genes kbaY kbaZ,also called agaY agaZ). The two al...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0406-6
更新日期:2002-05-01 00:00:00
abstract::A combination of gene loss and acquisition through horizontal gene transfer (HGT) is thought to drive Streptococcus thermophilus adaptation to its niche, i.e. milk. In this study, we describe an in silico analysis combining a stochastic data mining method, analysis of homologous gene distribution and the identificatio...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-010-0671-8
更新日期:2011-04-01 00:00:00
abstract::The Streptomyces virginiae gamma-butyrolactone autoregulator virginiae butanolide is a low-molecular-weight Streptomyces hormone eliciting virginiamycin biosynthesis through its binding to the specific receptor protein, BarA. Immediately downstream of barA lies barB, the transcription of which is tightly repressed by ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0625-5
更新日期:2004-01-01 00:00:00
abstract::Streptococcus intermedius is a causative agent of brain or liver abscesses. S. intermedius produces intermedilysin that plays a pivotal role in pathogenicity. We identified other pathogenic factors and described a fibronectin binding protein (FBP) homolog of S. intermedius (FbpI) that mediated bacterial adhesion to ep...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01922-y
更新日期:2020-10-01 00:00:00
abstract::A novel Gram-negative rod, endophytic bacterium, designated strain TMCC 8258T, was isolated from the root of Camellia sinensis collected from Puer, south-west China. Comparative 16S rRNA gene sequence analysis showed that the strain belongs to the family Sphingobacteriaceae and a neighbour-joining phylogenetic tree su...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-017-1391-0
更新日期:2017-11-01 00:00:00
abstract::Recently, we reported the synthesis of five bacteriocin-like inhibitor substances (Bt-BLIS: morricin 269, kurstacin 287, kenyacin 404, entomocin 420, and tolworthcin 524) by Mexican strains of Bacillus thuringiensis. Here we show that, collectively, these Bt-BLIS have a moderate to broad spectrum of antibacterial acti...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-008-0414-2
更新日期:2008-12-01 00:00:00
abstract::In phototrophic and chemoautotrophic proteobacteria, genes encoding enzymes of the Calvin-Benson-Bassham pathway of CO2 fixation are often found in clusters that are transcribed from a single promoter under control of the LysR-type transcriptional activator, CbbR. Mutations affecting CbbR prevent induction of cbb gene...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s002030050369
更新日期:1996-09-01 00:00:00
abstract::The disaccharide sophorose induces Trichoderma to increase a soluble intracellular b-glucosidase that hydrolyses cellobiose, sophorose, and p-nitrophenyl-b-D-glucopyranoside. Simultaneously, it depresses the activity of a similar insoluble enzyme that is associated with the mycelium. Gel electrophoresis indicates that...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425807
更新日期:1984-01-01 00:00:00
abstract::An ammonia-oxidizing, autotroph growing, slender, curved rod was isolated from the soil of Hawaii. It is well distinguishable from any other nitrifying bacteria thus far described by their morphology. The cells are 1.1-3.0 mum long and 0.3-0.4 mum wide. They are motile by means of 1-4 subpolar to lateral flagella. In ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425099
更新日期:1976-05-03 00:00:00
abstract::Carbon-14 was incorporated into oxalate and CO2 from either citrate-1,5-14C, succinate-1,4-14C, or fumarate-1,4-14C by cultures of Aspergillus niger pregrown on a medium which contained glucose as the sole carbon source and which did not allow citrate accumulation. In cell-free extracts of mycelium forming oxalate and...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00447318
更新日期:1975-06-22 00:00:00
abstract::It was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus Frankia. Recently, the number of nitrogen fixation (nifH) genes identified in other non-Frankia actinobacteria has dramatically increased and has open...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-011-0733-6
更新日期:2012-01-01 00:00:00