Abstract：
:We describe a convenient method for the in vivo construction of large plasmids that possess a multitude of restriction sites. A large (23 kbases) circular self-replicating plasmid carrying a partial LEU2-d gene was cotransformed with a circular non-replicating plasmid carrying the entire LEU2 gene. In vivo recombination results preferentially in a plasmid that carries both the LEU2-d and the entire LEU2 gene. In addition we also found one plasmid with a tandem LEU2 insertion and one plasmid where the LEU2-d gene was replaced by the entire LEU2 gene.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Valinger R,Braus G,Niederberger P,Künzler M,Paravicini G,Schmidheini T,Hütter Rdoi
10.1007/BF00409661subject
Has Abstractpub_date
1989-01-01 00:00:00pages
263-8issue
3eissn
0302-8933issn
1432-072Xjournal_volume
152pub_type
杂志文章abstract：:Mangrove sediment-associated bacteria are of significantly important in the field of medicine and pharmaceuticals as new promising sources of biologically active pharmacophores due to the extreme conditions, such as high salt concentration and soil anoxia. The sediment bacteria associated with Acanthus ilicifolius and...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01999-5
更新日期：2021-01-01 00:00:00
abstract：:It was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus Frankia. Recently, the number of nitrogen fixation (nifH) genes identified in other non-Frankia actinobacteria has dramatically increased and has open...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-011-0733-6
更新日期：2012-01-01 00:00:00
abstract：:Chemoautotrophic endosymbionts residing in Solemya velum gills provide this shallow water clam with most of its nutritional requirements. The cbb gene cluster of the S. velum symbiont, including cbbL and cbbS, which encode the large and small subunits of the carbon-fixing enzyme ribulose 1,5-bisphosphate carboxylase/o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0689-x
更新日期：2004-09-01 00:00:00
abstract：:The fate of 35S label was studied during cystine degradation by mycelia of the dermatophytic fungus Microsporum gypseum. Excess free cystine in the medium was readily taken up and its sulfur moiety excreted as inorganic sulfate and sulfite. At intervals after 3-60 min of incubation with 35S cystine the products of cys...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446777
更新日期：1986-07-01 00:00:00
abstract：:To clarify the deactivation mechanism of pyruvate formate-lyase (PFL) and its role in the regulation of fermentation in Streptococcus bovis, the molecular properties and genetic expression of multifunctional alcohol dehydrogenase (ADHE) were investigated. S. bovis was found to have ADHE, which was deduced to consist o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0638-0
更新日期：2004-02-01 00:00:00
abstract：:A lack on the association between acid tolerance response (ATR) and osmotolerance response (OTR) among Listeria monocytogenes dairy isolates was found. In order to evaluate how wild L. monocytogenes isolates mount tolerance responses under a sub-lethal pH and a low sodium chloride concentration (pH 5.5 and 3.5 % [w/v]...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0878-6
更新日期：2013-05-01 00:00:00
abstract：:To confirm the existence in nature of Salmonella enteritidis strains of different degrees of virulence and to elucidate the mechanisms underlying the effects of such strains on the epithelial barrier function, the consequences of infection of Caco-2 cells and HEp-2 cells with 15 S. enteritidis strains in a chicken inf...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030000236
更新日期：2001-01-01 00:00:00
abstract：:The TTA codon, one of the six available codons for the amino acid leucine, is the rarest codon among the high GC genomes of Actinobacteria including Frankia. This codon has been implicated in various regulatory mechanisms involving secondary metabolism and morphological development. TTA-mediated gene regulation is wel...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0731-8
更新日期：2012-01-01 00:00:00
abstract：:Aspergillus nidulans is able to grow on oleic acid as sole carbon source. Characterization of the oleate-induced beta-oxidation pathway showed the presence of the two enzyme activities involved in the first step of this catabolic system: acyl-CoA oxidase and acyl-CoA dehydrogenase. After isopicnic centrifugation in a ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050392
更新日期：1996-11-01 00:00:00
abstract：:Expression of the gene encoding ribosome modulation factor (RMF), as measured using an rmf-lacZ gene fusion, increased with decreasing pH in exponential phase cultures of Escherichia coli. Expression was inversely proportional to the growth rate and independent of the acidifying agent used and it was concluded that ex...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0025-0
更新日期：2005-10-01 00:00:00
abstract：:Of 147 microorganisms isolated from a loamy sand, 71 showed good growth with lindane (gamma-1,2,3,4,5,6-hexachlorocyclohexane) and produced chloride in an aqueous medium. Thirteen soil microorganisms were selected to study the utilization of lindane. Lindane was metabolized by the microbes to gamma-2,3,4,5,6-pentachlo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454850
更新日期：1976-07-01 00:00:00
abstract：:After the addition to soil of large numbers of a cowpea Rhizobium strain, the population declined steadily until the numbers reached about 10(7)/g, and the protozoa rose to about 10(4)/g. When indigenous protozoa were suppressed by the addition of actidione to the soil, the density of the test rhizobium did not fall i...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00492022
更新日期：1977-06-20 00:00:00
abstract：:For the low molecular weight laccases II and III of Podospora anserina the kinetic parameters Michaelis constant (KM) and maximum reaction velocity (V) were determined polarographically under pH optimum conditions for representative substrates of different substitution patterns. Laccase II showed two peaks in its pH o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446663
更新日期：1977-02-04 00:00:00
abstract：:In this study, a Candida digboiensis strain was isolated from a heap leaching plant in Zambia and used in double-layer agar plate to efficiently isolate and purify leaching bacteria. Unlike Acidiphilium sp., the yeast strain was tetrathionate tolerant and could metabolize a great range of organic compounds including o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-1051-6
更新日期：2015-03-01 00:00:00
abstract：:1. With fumarate as the terminal electron acceptor and either H2 or formate as donor, Vibrio succinogenes could grow anaerobically in a mineral medium using fumarate as the sole carbon source. Both the growth rate and the cell yield were increased when glutamate was also present in the medium. 2. Glutamate was incorpo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00405882
更新日期：1982-05-01 00:00:00
abstract：:Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, one of the most widespread and destructive bacterial diseases in rice. This study identified and characterized the contribution of the twin-arginine translocation (Tat) pathway to motility, chemotaxis, extracellular polysaccharide (EPS) production and virulen...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0440-0
更新日期：2009-02-01 00:00:00
abstract：:Mutants resistance to various combinations of threonine, lysine and/or their analogs were obtained and characterized in Pseudomonas acidovorans. In particular, mutants resistant to aminoethylcysteine had a dihydrodipicolinate synthetase insensitive to lysine inhibition whereas mutants resistant to threonine plus a low...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446845
更新日期：1976-03-19 00:00:00
abstract：:The occurrence of emodin, erythroglaucin, physcion, physcion-9-anthrone, questin, catenarin, and catenarin-8-methyl ether in different species of the Aspergillus glaucus group (genus Eurotium) was investigated. So far catenarin-8-methyl ether (1, 4, 6-trihydroxy-8-methoxy-3-methylanthraquinone) has not been described ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00409924
更新日期：1980-07-01 00:00:00
abstract：:Methanolic extract of Artemisia pallens (MEAP) (Asteraceae) was explored as greenbiocorrosion inhibitor for mild steel 1010 in 1.5% sodium chloride environment. Bacillus megaterium SKR7 induces the development of biofilm on the metal surface and forms the pitting corrosion. MEAP was showed (25 ppm) optimum inhibition ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01951-7
更新日期：2020-10-01 00:00:00
abstract：:The filamentous fungus Penicillium italicum when grown in a synthetic medium, produced and secreted 1,6-beta-glucanase into the culture medium. This enzyme has been partially purified by gel filtration. After this step the active fractions were free of 1,3-beta-glucanase, alpha-amylase and beta-glucosidase activities....
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425066
更新日期：1979-06-01 00:00:00
abstract：:Saccharomyces cerevisiaepresents two alternative vegetative forms of growth, switching between yeast forms to pseudohyphal forms depending on the specific environmental conditions. To identify genes involved in cell wall morphogenesis, a haploid S. cerevisiae monomorphic mutant, W27, which exhibits pseudohyphal growth...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0400-z
更新日期：2002-04-01 00:00:00
abstract：:Calcium was found to stimulate stalk development in Caulobacter crescentus and to relieve the inhibition of development long known to be caused by phosphate. This suggested that phosphate inhibition could be attributed to its interaction with Ca2+, thereby depriving the cells of a factor that promoted development. Cal...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413014
更新日期：1984-06-01 00:00:00
abstract：:Recently, it was proposed that some antibiotics stimulate the production of reactive oxygen species (ROS), which contribute to cell death. Later, other research groups have provided arguments against ROS-mediated killing of bacteria by antibiotics. At present, there remain a number of unanswered questions in understan...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1247-z
更新日期：2016-11-01 00:00:00
abstract：:The interaction between Shigella dysenteriae or Shigella sonnei and Acanthamoeba castellanii was studied by viable counts, gentamicin assay and electron microscopy. The result showed that Shigella dysenteriae or Shigella sonnei grew and survived in the presence of amoebae for more than 3 weeks. Gentamicin assay showed...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0422-2
更新日期：2009-01-01 00:00:00
abstract：:The alpha-glucosidase (alpha-D-glucoside glucohydrolase, EC 3.2.1.20) of Pseudomonas fluorescens W was partially purified by (NH4)2SO4 fractionation, Sephadex G-200 and DEAE-cellulose column chromatography. The enzyme showed great specificity for maltose hydrolysis, with very little action against polymeric forms. Suc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425338
更新日期：1976-04-01 00:00:00
abstract：:We previously cloned the sigH gene encoding a stress-response sigma factor sigma(H) in Streptomyces coelicolor A3(2), located in an operon with the gene encoding proposed anti-sigma factor UshX. To clarify the in vivo function of sigma(H), a stable null mutant of sigH was prepared by homologous recombination. This mut...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-001-0367-1
更新日期：2001-12-01 00:00:00
abstract：:Acinetobacter calcoaceticus LMD 79.41 produced significant amounts of pyrrolo-quinoline quinone (PQQ) in its culture medium when grown on quinic acid or shikimic acid. Studies with LMD 79.41 and PQQ- -mutants of this strain demonstrated that this organism contains an NAD(P)-independent quinate dehydrogenase (QDH) (EC ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00409714
更新日期：1988-05-01 00:00:00
abstract：:The nitrite oxidoreductase (NOR) from the facultative nitrite-oxidizing bacterium Nitrobacter hamburgensis X14 was investigated genetically. In order to develop a probe for the gene norB, the N-terminal amino acid sequence of the NOR beta-subunit (NorB) was determined. Based on that amino acid sequence, an oligonucleo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245305
更新日期：1993-01-01 00:00:00
abstract：:Mannoproteins from cell walls of Saccharomyces cerevisiae synthesized at successive stages of the population growth cycle have been solubilized with Zymolyase and subsequently analyzed. The major change along the population cycle concerned a large size mannoprotein material; the size of the newly-synthesized molecules...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425354
更新日期：1987-07-01 00:00:00
abstract：:1. Culture filtrates of heterotrophic bacteria were tested for their stimulatory effect on nitrification of three strains of Nitrobacter. 2. Yeast extract-peptone solution, in which Pseudomonas fluorescens had grown, after removal of the cells was added to autotrophically growing cultures of Nitrobacter agilis; it cau...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454856
更新日期：1976-07-01 00:00:00
