Abstract：
:Monoclonal antibodies have been used to determine the presence of cellobiohydrolases I and II (CBH I and II), and endoglucanase I (EG I) on the surface of conidia from Trichoderma reesei QM 9414 and RUT C-30, and 8 other Trichoderma species. For this purpose, proteins were released from the conidial surface by treatment with a non-ionic detergent (Triton X-100 and beta-octylglucoside), followed by SDS-PAGE/Western blotting and immunostaining. Both CBH I and II were clearly present, but - unlike in extracellular culture fluids from Trichoderma - CBH II was the predominant cellulase. In T. reesei EG I could not be detected. The higher producer strain T. reesei RUT C-30 exhibited a higher conidial level of CBH II than T. reesei QM 9414. In order to assess the importance of the conidial CBH II level for cellulase induction by cellulose, multiple copies of the chb2 gene were introduced into the T. reesei genome by cotransformation using PyrG as a marker. Stable multicopy transformants secreted the 2- to 4-fold level of CBH II into the culture medium when grown on lactose as a carbon source, but their CBH I secretion was unaltered. Upon growth on cellulose, both CBH I and CBH II secretion was enhanced. Those strain showing highest cellulase activity on cellulose also appeared to contain the highest level of conidial bound CBH II. CBH II was also the predominant conidial cellulase in various other Trichoderma sp. However, roughly the same amount of conidial bound CBH II was detected in all strains, although their cellulase production differed considerably.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Messner R,Kubicek-Pranz EM,Gsur A,Kubicek CPdoi
10.1007/BF00245356subject
Has Abstractpub_date
1991-01-01 00:00:00pages
601-6issue
6eissn
0302-8933issn
1432-072Xjournal_volume
155pub_type
杂志文章abstract：:A highly efficient method of transposon mutagenesis was developed for genetic analysis of Xanthobacter autotrophicus Py2. The method makes use of a transposon delivery vector that encodes a hyperactive Tn 5 transposase that is 1,000-fold more active than the wild-type transposase. In this construct, the transposase is...
journal_title：Archives of microbiology
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pub_type： 杂志文章
doi：10.1007/BF00429118
更新日期：1978-09-01 00:00:00
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更新日期：1998-04-01 00:00:00
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doi：10.1007/s00203-003-0593-9
更新日期：2003-10-01 00:00:00
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doi：10.1007/s00203-003-0615-7
更新日期：2004-01-01 00:00:00
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更新日期：1997-09-01 00:00:00
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doi：10.1007/s002039900125
更新日期：2000-03-01 00:00:00
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更新日期：2001-07-01 00:00:00
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journal_title：Archives of microbiology
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更新日期：1977-04-01 00:00:00
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journal_title：Archives of microbiology
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journal_title：Archives of microbiology
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doi：10.1007/BF00447315
更新日期：1975-06-22 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：2020-10-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050360
更新日期：1996-08-01 00:00:00
abstract：:Fermentative degradation of phenol was studied using a non-methanogenic, pasteurised enrichment culture containing two morphologically different bacteria. Phenol was fermented to benzoate, acetate and butyrate and their relative occurrence depended on the concentration of hydrogen. Proportionately more benzoate was fo...
journal_title：Archives of microbiology
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更新日期：2000-05-01 00:00:00
