Abstract:
:A highly efficient method of transposon mutagenesis was developed for genetic analysis of Xanthobacter autotrophicus Py2. The method makes use of a transposon delivery vector that encodes a hyperactive Tn 5 transposase that is 1,000-fold more active than the wild-type transposase. In this construct, the transposase is expressed from the promoter of the tetA gene of plasmid RP4, which is functional in a wide variety of organisms. The transposon itself contains a kanamycin resistance gene as a selectable marker and the origin of replication from plasmid R6K to facilitate subsequent cloning of the resulting insertion site. To test the effectiveness of this method, mutants unable to produce the characteristic yellow pigment (zeaxanthin dirhamnoside) of X. autotrophicus Py2 were isolated and analyzed. Transposon insertions were obtained at high frequency: approximately 1 x 10(-3) per recipient cell. Among these, pigment mutants were observed at a frequency of approximately 10(-3). Such mutants were found to have transposon insertions in genes homologous to known carotenoid biosynthetic genes previously characterized in other pigmented bacteria. Mutants were also isolated in Pseudomonas stutzeri and in an Alcaligenes faecalis, demonstrating the effectiveness of the method in diverse Proteobacteria. Preliminary results from other laboratories have confirmed the effectiveness of this method in additional phylogenetically diverse species.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Larsen RA,Wilson MM,Guss AM,Metcalf WWdoi
10.1007/s00203-002-0442-2keywords:
subject
Has Abstractpub_date
2002-09-01 00:00:00pages
193-201issue
3eissn
0302-8933issn
1432-072Xjournal_volume
178pub_type
杂志文章abstract::Hopane glycolipid, isolated from Bacillus acidocaldarius, was studied in its ability to support growth from Mycoplasma mycoides var. capri as substitute for sterols. All attempts to culture Mycoplasma on hopane glycolipid failed. When added together with cholesterol, the effect of HGL was a reduction in growth rate. T...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00413519
更新日期:1982-12-01 00:00:00
abstract::Exoglucanase (exo-1,3-beta-D-glucan glycohydrolase, EC 3.2.1.56) activity secreted by Saccharomyces cerevisiae into the culture medium was separated by ion exchange chromatography into two glycoprotein isoenzymes which contributed 10% (exoglucanase I) and 90% (exoglucanase II) towards the total activity. Analysis of t...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00416596
更新日期:1989-01-01 00:00:00
abstract::Small heat-shock proteins are molecular chaperones that bind and prevent aggregation of nonnative proteins. They also associate with membranes. In this study, we show that the small heat-shock protein HspA plays a protective role under oxidative stress in the cyanobacterium Synechococcus elongatus strain ECT16-1, whic...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-009-0457-z
更新日期:2009-04-01 00:00:00
abstract::In yeasts, several sensing systems localized to the plasma membrane which transduce information regarding the availability and quality of nitrogen and carbon sources and work in parallel with the intracellular nutrient-sensing systems, regulate the expression and activity of proteins involved in nutrient uptake and ut...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0022-3
更新日期:2005-11-01 00:00:00
abstract::An Arthrobacter sp. growing on 4-Chlorobenzoic acid as its sole source of carbon excretes 4-hydroxygenzoic acid and protocatechuic acid into the culture medium. Protocatechuic acid is further attacked by "meta"-cleavage. During growth of the Arthrobacter sp. on benzoic acid cis-cis muconic acid can be isolated from th...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00690235
更新日期:1976-11-02 00:00:00
abstract::The diversity among 45 cyanobacterial isolates from 11 different Gunnera species originating from different geographical areas was examined. By means of polymerase chain reaction (PCR) fingerprinting with short tandemly repeated repetitive (STRR) sequences as primers, ten groups of symbiotic cyanobacteria and five uni...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002039900113
更新日期:2000-02-01 00:00:00
abstract::Differences in the rumen bacterial community have been previously reported for Soay sheep housed under different day length conditions. This study extends this previous investigation to other organs of the digestive tract, as well as the analysis of ciliated protozoa and anaerobic fungi. The detectable concentrations ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01652-w
更新日期:2019-09-01 00:00:00
abstract::The growth, duplication and fate of multikaryotic hyphae bearing true clamp connections, as derived from compatible matings of Schizophyllum commune, were studied by phase contrast microscopy. The nuclei (N) of multikaryotic apices maintained a near central position during hyphal growth. True clamp connection formatio...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00406155
更新日期:1980-12-01 00:00:00
abstract::A new method is described for the quantitative microbiological analysis of the thiamine analog pyrithiamine present in biological material. The method uses a mutant of Lactobacillus fermenti (ATCC 9338) stimulated by pyrithiamine. By this specific method it is found that Serratia marcescens is able to consume and to p...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00436334
更新日期:1975-03-12 00:00:00
abstract::We investigated the structural and immunochemical characteristics of cell wall mannan obtained from Candida sojae JCM 1644, which is a new yeast species isolated from defatted soybean flakes. The results of a slide-agglutination test and of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0339-1
更新日期:2008-05-01 00:00:00
abstract::The disaccharide sophorose induces Trichoderma to increase a soluble intracellular b-glucosidase that hydrolyses cellobiose, sophorose, and p-nitrophenyl-b-D-glucopyranoside. Simultaneously, it depresses the activity of a similar insoluble enzyme that is associated with the mycelium. Gel electrophoresis indicates that...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425807
更新日期:1984-01-01 00:00:00
abstract::The major cobamide-containing protein from methanol-utilizing Sporomusa ovata was 8-fold enriched to apparent homogeneity. The protein exhibited a molecular mass of 40 kDa and of 38 kDa determined by gel filtration and by SDS-polyacrylamide gel electrophoresis, respectively. This finding indicates a monomeric protein ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00245367
更新日期:1992-01-01 00:00:00
abstract::The proteins in the culture supernatant (exoproteins) from Streptococcus pyogenes serotype M1 were separated by two-dimensional gel electrophoresis, and their N-terminal amino acid sequences were determined. The amino acid sequences were compared to sequences in the S. pyogenes genome database. The coding sequence sho...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0412-8
更新日期:2002-06-01 00:00:00
abstract::The mechanism of the massive extracellular production of citric and isocitric acids by Saccharomycopsis lipolytica grown on n-paraffins has been studied. When growth stops, because of nitrogen limitation, the intracellular concentration of ATP sharply rises whereas that of AMP and ADP decreases to a low level. At the ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00428588
更新日期:1977-05-13 00:00:00
abstract::The induction of the enzyme activities involved in butane metabolism in Pseudomonas butanovora was characterized. P. butanovora was grown on butane or its metabolites, both singly and in mixtures with other growth substrates. Cells grown in each of the butane metabolites readily consumed the growth substrate and downs...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030100304
更新日期:2001-07-01 00:00:00
abstract::Under starvation conditions, rhodopsin-containing halobacteria show a light-accelerated death under aerobic conditions. This is attributed to photooxidative processes. Under anaerobic conditions, halobacteria die rapidly in the dark, and light prevents death. Since it has been shown by others that lightdriven ATP synt...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425136
更新日期:1976-08-01 00:00:00
abstract::Recent studies indicated that bioluminescence of the marine bacterium Vibrio harveyi may both stimulate DNA repair and contribute to detoxification of deleterious oxygen derivatives. Therefore, it was also proposed that these reactions can be considered biological roles of bacterial luminescence and might act as evolu...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0764-y
更新日期:2005-03-01 00:00:00
abstract::Candida utilis grew on ethanol and an ethanol-isopropanol-water (22:2:1 vols) mixture but not on isopropanol alone. Acetone accumulated in all cultures containing isopropranol but its presence in the alcohol mixture did not lower growth rate or yield significantly when compared with growth experiments on ethanol alone...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00403209
更新日期:1980-03-01 00:00:00
abstract::The cold shock response in bacteria involves the expression of low-molecular weight cold shock proteins (CSPs) containing a nucleic acid-binding cold shock domain (CSD), which are known to destabilize secondary structures on mRNAs, facilitating translation at low temperatures. Caulobacter crescentus cspA and cspB are ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-010-0602-8
更新日期:2010-09-01 00:00:00
abstract::An anaerobic, mesophilic, syntrophic benzoate-degrading bacterium, designated strain FB(T), was isolated from methanogenic sludge which had been used to treat wastewater from the manufacture of terephthalic acid. Cells were non-motile gram-positive rods that formed spores. The optimum temperature for growth was 35-40 ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0521-z
更新日期:2003-04-01 00:00:00
abstract::The polymorphic GC-rich repetitive sequence (PGRS) found on the chromosome of Mycobacterium tuberculosis was characterized by means of mapping, cloning and sequencing. PGRS was present in at least 26 loci and consisted of many tandem repeats of the consensus sequence CGGCGGCAA. As the core of the consensus motif was t...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00381781
更新日期:1995-02-01 00:00:00
abstract::Comparative 16S rRNA gene sequence and genomic DNA reassociation analyses were used to assess the phylogenetic relationships of Methanobrevibacter fecal isolates. The 16S rRNA gene sequences of Methanobrevibacter smithii strain PS and the human fecal isolates B181 and ALI were essentially identical, and their genomic ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050589
更新日期:1998-05-01 00:00:00
abstract::Bacteriophages rapidly diversify their genes through co-evolution with their hosts. We hypothesize that gene diversification of phages leads to locality in phages genome. To test this hypothesis, we investigated the genetic diversity and composition of Microcystis cyanophages using 104 sequences of Ma-LMM01-type cyano...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-0980-4
更新日期:2014-06-01 00:00:00
abstract::Although, there have been many published bacterial strains aerobically degrading the heterocyclic amine compounds, only one strain to date has been reported to degrade pyrrolidine under denitrifying conditions. In this study, denitrifying bacteria degrading pyrrolidine and piperidine were isolated from diverse geologi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-008-0452-9
更新日期:2009-04-01 00:00:00
abstract::Sporothrix schenckii is the etiological agent of sporotrichosis, a mycosis of humans and other mammals. Little is known about the responses of this thermodimorphic pathogen to perturbations in the cell wall (CW) by different stress conditions. Here we describe the effect of Congo Red (CR) on the fungal growth, morphog...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-018-1576-1
更新日期:2019-01-01 00:00:00
abstract::Polyaromatic hydrocarbons (PAHs) are considered as hazardous organic priority pollutants. PAHs have immense public concern and critical environmental challenge around the globe due to their toxic, carcinogenic, and mutagenic properties, and their ubiquitous distribution, recalcitrance as well as persistence in environ...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-020-01929-5
更新日期:2020-10-01 00:00:00
abstract::Outer membrane vesicles (OMVs) are released from the outer membrane of Gram-negative bacteria. Moreover, Gram-positive bacteria also produce membrane-derived vesicles. As OMVs transport several bacterial components, especially from the cell envelope, their interaction with the host cell, with other bacteria or as immu...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-014-1042-7
更新日期:2015-01-01 00:00:00
abstract::Penicillium marneffei is a dimorphic fungus that intracellularly infects the reticuloendothelial system of humans and bamboo rats. Endemic in Southeast Asia, it infects 10% of AIDS patients in this region. The absence of a sexual stage and the highly infectious nature of the mould-phase conidia have impaired studies o...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0533-8
更新日期:2003-05-01 00:00:00
abstract::The fate of 35S label was studied during cystine degradation by mycelia of the dermatophytic fungus Microsporum gypseum. Excess free cystine in the medium was readily taken up and its sulfur moiety excreted as inorganic sulfate and sulfite. At intervals after 3-60 min of incubation with 35S cystine the products of cys...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446777
更新日期:1986-07-01 00:00:00
abstract::In order to obtain strains of Saccharomycopsis lipolytica impaired in the active transport of L-lysine, mutants resistant to a mixture of L-canavanine, L-4-5-transdehydrolysine and L-S-amino ethylcysteine, taken either all three or two by two, were isolated. These compounds were shown previously to be competitive inhi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00411361
更新日期:1979-08-06 00:00:00