Abstract：
:NAD kinase was purified 180-fold from Bacillus licheniformis to determine the role it plays in NADP turnover in this organism. The enzyme was found to have a pH optimum of 6.8 and an apparent Km for NAD of 2.7 mM. The ATP saturation curve was not hyperbolic; 5.5 mM ATP was required to reach half maximal activity. Both Mn2+ and Ca2+ could be substituted for Mg2+. Several compounds including nicotinic acid, nicotinamide, nicotinamide mononucleotide, quinolinic acid, NADPH, ADP, AMP and cyclic AMP did not affect NAD kinase activity. In contrast, the enzyme was inhibited by NADP at concentrations typically found in logarithmic cells of B. licheniformis. This inhibition was competitive with NAD and had a Ki of 0.13 mM. It is suggested that in vivo NAD kinase activity is highly dependent on the concentrations of NAD and ATP and the proportion of oxidized and reduced NADP.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Zerez CR,Moul DE,Andreoli AJdoi
10.1007/BF00409878subject
Has Abstractpub_date
1986-05-01 00:00:00pages
313-6issue
4eissn
0302-8933issn
1432-072Xjournal_volume
144pub_type
杂志文章abstract：:The induction by D,L-nicotine of the enantiozymes 6-hydroxy-L-nicotine oxidase and 6-hydroxy-D-nicotine oxidase in Arthrobacter oxidans was differently affected by the inhibitors of Escherichia coli gyrase, novobiocin and nalidixic acid. These compounds inhibited 6-hydroxy-L-nicotine oxidase induction slightly, but le...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00521289
更新日期：1982-12-03 00:00:00
abstract：:Colistin is a mixture of polymyxin E1 and E2, bactericidal pentacationic lipopeptides used to treat infections caused by Gram-negative pathogens such as Pseudomonas aeruginosa and Klebsiella pneumoniae. Industrial production of colistin is obtained by a fermentation process of the natural producer Paenibacillus polymy...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1084-5
更新日期：2015-05-01 00:00:00
abstract：:Mesorhizobium tianshanense is a nitrogen-fixing bacterium that can establish symbiotic associations with Glycyrrhiza uralensis in the form of root nodules. Nodule formation in rhizobia often requires various secreted carbohydrates. To investigate exopolysaccharide (EPS) production and function in M. tianshanense, we p...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0345-3
更新日期：2008-05-01 00:00:00
abstract：:Carbon-14 was incorporated into oxalate and CO2 from either citrate-1,5-14C, succinate-1,4-14C, or fumarate-1,4-14C by cultures of Aspergillus niger pregrown on a medium which contained glucose as the sole carbon source and which did not allow citrate accumulation. In cell-free extracts of mycelium forming oxalate and...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447318
更新日期：1975-06-22 00:00:00
abstract：:A high-efficiency fluoranthene-degrading bacterium Paenibacillus sp. PRNK-6 was isolated from PAH-contaminated soil. The strain degrades 96% (240 mg l-1) of fluoranthene in 48 h. Various metabolic intermediates of fluoranthene catabolism were identified by gas chromatography (GC) and gas chromatography-high resolution...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1431-9
更新日期：2018-01-01 00:00:00
abstract：:Glucose and other sugars, such as galactose or maltose, are able to cause carbon catabolite repression in Saccharomyces cerevisiae. Although glycolytic intermediates have been suggested as signal for repression, no evidence for such a control mechanism is available. The establishment of a correlation between levels of...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0593-9
更新日期：2003-10-01 00:00:00
abstract：:All Serratia marcescens strains (total of 33) of different sources were hemolytic including clinical strains previously classified as being nonhemolytic. DNA fragments of the two hemolysin genes hybridized with the chromosomal DNA of S. marcescens, S. liquefaciens, S. kiliensis, S. grimesii, S. proteamaculans, S. plym...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00248958
更新日期：1990-01-01 00:00:00
abstract：:Type I signal peptidases are responsible for the proteolytic cleavage of the signal peptide of secreted proteins. In the gram-positive bacterium Streptomyces lividans, four adjacent genes (sipW, sipX, sipY and sipZ) were isolated encoding putative type I signal peptidases. In this work, the different sip genes were cl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100335
更新日期：2001-11-01 00:00:00
abstract：:The divergently transcribed sulfur oxidation (sox) operon of a sulfur chemolithotrophs, Pseudaminobacter salicylatoxidans KCT001, comprising sox TRS-VW-XYZABCD, is regulated by a repressor (SoxR). SoxR binds to two disparate operators, sv (present in between soxS and soxV) and wx (present in between soxW and soxX). He...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0808-z
更新日期：2012-09-01 00:00:00
abstract：:A fromaldehyde oxidase activity was found in cellfree extracts of methanol-grown yeast Candida boidinii. Loss of alcohol oxidase activity in a mutant, 48, led to loss of the formaldehyde oxidase activity, indicating that the same enzyme is probably responsible for both activities. This could be demonstrated with the p...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447134
更新日期：1975-10-27 00:00:00
abstract：:The tricarboxylic acid (TCA) cycle is one of the major routes of carbon catabolism in Bacillus subtilis. The syntheses of the enzymes performing the initial reactions of the cycle, citrate synthase, and aconitase, are synergistically repressed by rapidly metabolizable carbon sources and glutamine. This regulation invo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0078-0
更新日期：2006-03-01 00:00:00
abstract：:The synthesis of the luminous system of the marine luminous bacterium Photobacterium fischeri is subject to a complex, self-regulated control system called autoinduction. The bacteria produce an autoinducer which accumulates in the medium at a constant rate (as a function of cell growth). When autoinducer reaches a cr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446657
更新日期：1977-02-04 00:00:00
abstract：:The biosynthesis of the beta-glucosidase enzyme was studied in a transformed yeast obtained by cloning in Saccharomyces cerevisiae the structural gene coding for beta-glucosidase in Kluyveromyces fragilis. The enzyme biosynthesis was found to be non-adaptative, and repressed by glucose. These features are similar to t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00402336
更新日期：1986-11-01 00:00:00
abstract：:Lactic acid bacteria can be considered as natural biopreservative and good biotechnological alternative to food safety. In this study, the antilisterial compounds produced by Enterococcus isolates from the Patagonian environment and their effectiveness for the control of Listeria monocytogenes in a food model were stu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02118-0
更新日期：2021-01-02 00:00:00
abstract：:The diversity of eukaryotic populations, in particular protozoa, in the water supplies of intensively reared broilers has not been previously studied. This important food-rearing environment was screened for the molecular diversity of eukaryotes by the analysis of PCR-amplified 18S rRNA. DNA was extracted from filtere...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0119-3
更新日期：2006-07-01 00:00:00
abstract：:The disaccharide sophorose induces Trichoderma to increase a soluble intracellular b-glucosidase that hydrolyses cellobiose, sophorose, and p-nitrophenyl-b-D-glucopyranoside. Simultaneously, it depresses the activity of a similar insoluble enzyme that is associated with the mycelium. Gel electrophoresis indicates that...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425807
更新日期：1984-01-01 00:00:00
abstract：:Washed cells prepared from carbon-limited continuous cultures of Alcaligenes eutrophus synthesised poly-3-hydroxybutyrate (PHB) rapidly when supplied with glucose, DL-lactate or L-lactate. Unlike growing cultures, washed cells excreted significant amounts of pyruvate. The combined rates of PHB production (qPHB) and py...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050526
更新日期：1997-12-01 00:00:00
abstract：:Listeria monocytogenes was examined for the presence of surface carbohydrates to ascertain whether surface sugars, if present, would interact with eucaryotic surface carbohydrate receptors. We found that a virulent, but not two avirulent strains had a surface alpha-D-galactose residue as determined by agglutination wi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00249083
更新日期：1990-01-01 00:00:00
abstract：:In Lactobacillus casei BL23, phosphofructokinase activity was higher in cells utilizing sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system (PTS). The phosphofructokinase gene (pfk) was cloned from L. casei and shown to be clustered with the gene encoding pyruvate kinase (pyk). pfk and pyk ge...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0003-6
更新日期：2005-09-01 00:00:00
abstract：:A new psychrophilic, anaerobic, acetogenic bacterium from the tundra wetland soil of Polar Ural is described. The organism fermented H2/CO2, formate, methanol, and several sugars to acetate as the sole end-product. The temperature range for growth was 1-30 degrees C with an optimum at 20 degrees C. The bacterium showe...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030000229
更新日期：2000-12-01 00:00:00
abstract：:The haloalkaliphilic archaeon Natronobacterium vacuolatum forms cylinder-shaped gas vesicles throughout the growth cycle when grown in media containing 15-25% NaCl. Cells cultivated in media containing 13% NaCl are, however, gas-vesicle-free. The major gas vesicle structural protein, nv-GvpA, was detected by an antise...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050465
更新日期：1997-07-01 00:00:00
abstract：:Dunaliella tertiolecta (marine) and D. viridis (halophilic) were each trained by serial transfer to grow at salt concentrations previously regarded as the other's domain. D. viridis then had a salt optimum at 1.0-1.5 M sodium chloride whereas that for D. tertiolecta was less than 0-2 M. Nevertheless D. tertiolecta gre...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428592
更新日期：1977-05-13 00:00:00
abstract：:When Clostridium formicoaceticum was grown on fumarate or L-malate crude cell extracts contained a high fumarate reductase activity. Using reduced methyl viologen as electron donor the specific activity amounted to 2-3.5 U per mg of protein. Reduced benzyl viologen, FMNH2 and NADH could also serve as electron donors b...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00407920
更新日期：1978-10-04 00:00:00
abstract：:A bacterium capable of using the carbamate insecticide carbofuran as a sole source of carbon and energy, was isolated from soil. The ability to catabolise carbofuran phenol, produced by cleavage of the carbamate ester linkage of the insecticide, was lost at very high frequency when the bacterium was grown in the absen...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245249
更新日期：1992-01-01 00:00:00
abstract：:Differences in the rumen bacterial community have been previously reported for Soay sheep housed under different day length conditions. This study extends this previous investigation to other organs of the digestive tract, as well as the analysis of ciliated protozoa and anaerobic fungi. The detectable concentrations ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01652-w
更新日期：2019-09-01 00:00:00
abstract：:Histoplasma capsulatum yeast cells have been studied by immunoelectron microscopy using rabbit polyclonal antisera and a biotin-avidin-peroxidase detection system. An antigenic surface layer has been visualized in the cell wall of immunostained organisms. This layer was not seen in samples prepared by standard electro...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00693397
更新日期：1985-08-01 00:00:00
abstract：:During a screening program intended to identify genes encoding enzymes typical for secondary metabolism in Sorangium cellulosum So ce90, an aromatic amino acid decarboxylase gene (ddc) was detected. Expression of ddc in Escherichia coli and subsequent enzyme assays with cell-free extracts confirmed the proposed functi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002039900131
更新日期：2000-04-01 00:00:00
abstract：:The mechanism of the massive extracellular production of citric and isocitric acids by Saccharomycopsis lipolytica grown on n-paraffins has been studied. When growth stops, because of nitrogen limitation, the intracellular concentration of ATP sharply rises whereas that of AMP and ADP decreases to a low level. At the ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428588
更新日期：1977-05-13 00:00:00
abstract：:Aerobic anoxygenic phototrophs (AAPs) are prokaryotic microorganisms capable of harvesting light using bacteriochlorophyll-based reaction centres. Marine AAP communities are generally dominated by species belonging to the Roseobacter clade. For this reason, we used marine Roseobacter-related strain COL2P as a model or...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0529-0
更新日期：2010-01-01 00:00:00
abstract：:A gram-negative bacterium designated BN30(T), which is motile with a polar flagellum, non-endospores forming, oxidase- and catalase-positive, was isolated from soybean root nodule. The organism is facultative anaerobic and surface-wrinkled rod. It can grow at 10-40 °C, pH 6-8 and 6 % (w/v) NaCl. BLASTn search based on...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1102-7
更新日期：2015-06-01 00:00:00
