Detection of an antigenic cell wall layer in Histoplasma capsulatum. An immunoelectron microscopic study.

Abstract:

:Histoplasma capsulatum yeast cells have been studied by immunoelectron microscopy using rabbit polyclonal antisera and a biotin-avidin-peroxidase detection system. An antigenic surface layer has been visualized in the cell wall of immunostained organisms. This layer was not seen in samples prepared by standard electron microscopic methods or in negative controls used with the immunocytochemical technique. Without immunostaining the cell wall of Histoplasma appeared almost transparent. In contrast, after immunoperoxidase staining the cell wall was conspicuous, bounded by the darkly stained outer layer. This electron dense layer, appeared to be a reservoir of surface antigens that were recognized by anti-Histoplasma antibodies.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Edwards MR,Fritz KE

doi

10.1007/BF00693397

subject

Has Abstract

pub_date

1985-08-01 00:00:00

pages

242-7

issue

3

eissn

0302-8933

issn

1432-072X

journal_volume

142

pub_type

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