Abstract：
:When Clostridium formicoaceticum was grown on fumarate or L-malate crude cell extracts contained a high fumarate reductase activity. Using reduced methyl viologen as electron donor the specific activity amounted to 2-3.5 U per mg of protein. Reduced benzyl viologen, FMNH2 and NADH could also serve as electron donors but the specific activities were much lower. The NADH-dependent activity was strictly membrane-bound and rather labile. Its specific activity did not exceed 0.08 U per mg of particle protein. Fumarate reductase activity was also found in cells of C. formicoaceticum grown on fructose, gluconate, glutamate and some other substrates. The methyl viologen-dependent fumarate reductase activity could almost completely be measured with intact cells whereas only about 25% of the cytoplasmic acetate kinase activity was detected with cell suspensions. The preparation of spheroplasts from cells of C. formicoaceticum in 20 mM HEPES-KOH buffer containing 0.6 M sucrose and 1 mM dithioerythritol resulted in the specific release of 88% of the fumarate reductase activity into the spheroplast medium. Only small amounts of the cytoplasmic proteins malic enzyme and acetate kinase were released during this procedure. There results indicate a peripheral location of the fumarate reductase of C. formicoaceticum on the membrane.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Dorn M,Andreesen JR,Gottschalk Gdoi
10.1007/BF00407920subject
Has Abstractpub_date
1978-10-04 00:00:00pages
7-11issue
1eissn
0302-8933issn
1432-072Xjournal_volume
119pub_type
杂志文章abstract：:Fructose transport was not apparently affected in a number of Pseudomonas putida strains with deranged activity of a common glucose-gluconate uptake system, indicating the existence of an independent fructose uptake system. Fructose uptake by glucose-gluconate uptake mutants was induced by fructose and obeyed saturati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428362
更新日期：1975-01-01 00:00:00
abstract：:A new method is described for the quantitative microbiological analysis of the thiamine analog pyrithiamine present in biological material. The method uses a mutant of Lactobacillus fermenti (ATCC 9338) stimulated by pyrithiamine. By this specific method it is found that Serratia marcescens is able to consume and to p...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00436334
更新日期：1975-03-12 00:00:00
abstract：:Triacylglycerol granules synthesized and accumulated by Rhodococcus opacus and Rhodococcus ruber were isolated by glycerol density gradient centrifugation. Whereas only one type of granule could be isolated from R. opacus, two types of granules with different specific densities were isolated from R. ruber. Both types ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-001-0355-5
更新日期：2001-12-01 00:00:00
abstract：:Inhibitor studies and mutant analysis revealed a C(30) pathway via 4,4'-diapophytoene and 4,4'-diaponeurosporene to 4,4'-diaponeursoporene-4-oic acid esters related to staphyloxanthin in Halobacillus halophilus. Six genes may be involved in this biosynthetic pathway and could be found in two adjacent gene clusters. Tw...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0431-1
更新日期：2009-02-01 00:00:00
abstract：:Pandoraea sp. strain MCB032 was isolated as an emerging chlorobenzene degrader from a functionally stable bioreactor where species succession had occurred. In this study, two gene clusters encoding chlorobenzene metabolic functions have been cloned. Within the cbs gene cluster, CbsA and CbsB are similar to the chlorob...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0476-9
更新日期：2009-06-01 00:00:00
abstract：:Under starvation conditions, rhodopsin-containing halobacteria show a light-accelerated death under aerobic conditions. This is attributed to photooxidative processes. Under anaerobic conditions, halobacteria die rapidly in the dark, and light prevents death. Since it has been shown by others that lightdriven ATP synt...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425136
更新日期：1976-08-01 00:00:00
abstract：:The clones generated in a sequencing project represent a resource for subsequent analysis of the organism whose genome has been sequenced. We describe an interrelated group of cloning vectors that either integrate into the genome or replicate, and that enhance the utility, for developmental and other studies, of the c...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0276-z
更新日期：2007-12-01 00:00:00
abstract：:The free tryptophan pool and the levels of two enzymes of tryptophan biosynthesis (anthranilate synthase and indoleglycerolphosphate synthase)have been determined in a wild type strain of Saccharomyces cerevisiae and in mutants with altered regulatory properties. ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446842
更新日期：1976-03-19 00:00:00
abstract：:We previously constructed an arabinose-inducible recombinant Bifidobacterium longum that could efficiently express secreted IFN-alpha2b in vitro (Deng et al. in Arch Microbiol 191:681-686, 2009). Here, we investigated the influence of oral pBAD-SPIFN-transformed B. longum on immunomodulation and IFN-induced Mx1 gene t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0589-1
更新日期：2010-08-01 00:00:00
abstract：:A strictly anaerobic bacterium, strain PCE1, was isolated from a tetrachloroethene-dechlorinating enrichment culture. Cells of the bacterium were motile curved rods, with approximately four lateral flagella. They possessed a gram-positive type of cell wall and contained cytochrome c. Optimum growth occurred at pH 7.2-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050308
更新日期：1996-02-01 00:00:00
abstract：:Geobacter species such as G. bremensis, G. pelophilus, and G. sulfurreducens are obligately anaerobic and grow in anoxic, non-reduced medium by fast reduction of soluble ferric citrate. In contrast, insoluble ferrihydrite was either only slowly or not reduced when supplied as electron acceptor in similar growth experi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0686-0
更新日期：2004-10-01 00:00:00
abstract：:The mechanism of the massive extracellular production of citric and isocitric acids by Saccharomycopsis lipolytica grown on n-paraffins has been studied. When growth stops, because of nitrogen limitation, the intracellular concentration of ATP sharply rises whereas that of AMP and ADP decreases to a low level. At the ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428588
更新日期：1977-05-13 00:00:00
abstract：:The ability of soil microorganisms to solubilize phosphate is an important trait of plant growth-promoting bacteria leading to increased yields and smaller use of fertilizers. This study presents the isolation and characterization of phosphobacteria from Puna, northwestern Argentina and the ability to produce phosphat...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0692-y
更新日期：2011-07-01 00:00:00
abstract：:The regulatory elements involved in expression of the gene (fdhF) for the selenopolypeptide of formate dehydrogenase and of a gene (or transcriptional unit) (hyd) specifically responsible for the formation of the gas-evolving hydrogenase (hydrogenase 3) in Escherichia coli were investigated. Formate (or a product of i...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429646
更新日期：1987-06-01 00:00:00
abstract：:The diversity of eukaryotic populations, in particular protozoa, in the water supplies of intensively reared broilers has not been previously studied. This important food-rearing environment was screened for the molecular diversity of eukaryotes by the analysis of PCR-amplified 18S rRNA. DNA was extracted from filtere...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0119-3
更新日期：2006-07-01 00:00:00
abstract：:Planococcus halotolerans, recently described as a novel species with SCU63T as the type strain, is capable of thriving in up to 15% NaCl and temperatures as low as 0 °C. To better understand its adaptation strategies at the genomic level, strain SCU63T was subjected to whole-genome sequencing and data mining. The high...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01979-9
更新日期：2020-12-01 00:00:00
abstract：:The present study investigated the endophytic bacterial communities in the seeds of mature, natural common reed (Phragmites australis) and narrowleaf cattail (Typha angustifolia L.). Additionally, seed endophytic bacterial communities were compared with rhizospheric and root endophytic bacterial communities using Illu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-018-1493-3
更新日期：2018-08-01 00:00:00
abstract：:A Gram-reaction-negative, facultatively anaerobic bacterium, designated SAP-19T, was isolated from sap extracted from Acer pictum in Mt. Halla in Jeju, Republic of Korea and its taxonomic statue was investigated by a polyphasic approach including genome- and 16S rRNA gene-based phylogenetic analyses. Cells were motile...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01961-5
更新日期：2020-11-01 00:00:00
abstract：:Comparative 16S rRNA gene sequence and genomic DNA reassociation analyses were used to assess the phylogenetic relationships of Methanobrevibacter fecal isolates. The 16S rRNA gene sequences of Methanobrevibacter smithii strain PS and the human fecal isolates B181 and ALI were essentially identical, and their genomic ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050589
更新日期：1998-05-01 00:00:00
abstract：:Exogenous addition of adenosine- and guanosine 5'-(di- and tri) phosphate 3'-diphosphate (pppApp, ppApp, pppGpp and ppGpp) at the concentration of 0.5 mM inhibits spore germination of Streptomyces galilaeus ATCC 31133. This reversible inhibitory effect appeared to be at the transcriptional level, and also depends on t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446889
更新日期：1980-04-01 00:00:00
abstract：:Reciprocal qualitative and quantitative immunological experiments employing an anti-Pediococcus cerevisiae aldolase serum confirmed many of the interspecific relationships demonstrated previously among lactic acid bacteria with antisera prepared against the Streptococcus faecalis fructose diphosphate aldolase. The ext...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00416976
更新日期：1976-10-11 00:00:00
abstract：:Two proteases, designated I and II, have been isolated from sporulating cells of Bacillus subtilis. They were partially purified by ammonium sulfate fractionation, Sephadex chromatography and affinity columns. Protease I was found to be similar to an already characterized B. subtilis protease. Protease II is trypsin-l...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425256
更新日期：1981-05-01 00:00:00
abstract：:The utilization of carnitine and carnitine derivatives (O-acylcarnitines, carnitine carboxylderivatives) and structure-related trimethylammonium-compounds (betaines and nitrogen-bases) by Acinetobacter calcoaceticus was studied by means of the control of growth and the quantitative detection of metabolites. The strain...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429336
更新日期：1977-03-01 00:00:00
abstract：:Mercury resistance of Clostridium cochlearium T-2P was found to be controlled by a different mechanism from those reported so far since no mercury-reducing activity was detected in this strain. The H2S generating ability as well as the demethylating activity in this bacterium was eliminated by the treatment of the cur...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00417179
更新日期：1981-03-01 00:00:00
abstract：:Given the serious threat of foodborne multidrug-resistant bacteria to animals and humans, finding an effective antibacterial compound has always been an important topic for scientists. Here, from the soil of Changbaishan, we have identified a bacterium that can inhibit the growth of Staphylococcus aureus. Nr genome da...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02102-8
更新日期：2020-11-04 00:00:00
abstract：:The study focuses on analysis of the compositional and diversity of bacteria in termite mound soils in comparison with the surrounding soils to verify the assertion that the high nutrient concentrations in termite mound soils influence a complex diversity of microorganisms. Here, whole DNA was extracted from soil samp...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01994-w
更新日期：2020-12-01 00:00:00
abstract：:During cultivation of a gastric fungus, Coniochaeta polymorpha, growth of Nocardia colonies on top of the fungal culture raised the question whether bacteria originated from inside of fungus. In this study, the likelihood of intracellular origin of bacteria as well as interaction of two microorganisms was assessed. Fl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02122-4
更新日期：2021-01-02 00:00:00
abstract：:Aspergillosis causes high morbidity and mortality in avian species. The main goal of this study was to use molecular techniques to identify Aspergillus species collected from different avian species with aspergillosis. A subsample of those isolates was also screened for resistance to itraconazole. Over a 2-year period...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01618-y
更新日期：2019-03-01 00:00:00
abstract：:Studying the effects of microbial volatile organic compounds (VOCs) on plant growth is challenging because the production of volatiles depends on many environmental factors. Adding to this complexity, the method of volatile exposure itself can lead to different responses in plants and may account for some of the contr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1104-5
更新日期：2015-06-01 00:00:00
abstract：:Temperature dependency of sexual agglutination in Saccharomyces cerevisiae was found. Of 31 strains tested, which showed normal agglutination when cultured at 25 degrees C, 29 strains lost their sexual agglutinability when they were grown at 37 degrees C. ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446875
更新日期：1977-09-28 00:00:00
