Abstract：
:Type I signal peptidases are responsible for the proteolytic cleavage of the signal peptide of secreted proteins. In the gram-positive bacterium Streptomyces lividans, four adjacent genes (sipW, sipX, sipY and sipZ) were isolated encoding putative type I signal peptidases. In this work, the different sip genes were cloned and expressed. Subsequently, the Sip proteins were purified to raise antibodies. Although the four Sip proteins share a low degree of sequence similarity and differ significantly in size and pI, anti-Sip antibodies cross-reacted intensively. Functional signal peptidase processing activity for each of these Sip proteins was shown both in vitro and in vivo. The different Sip proteins did not exhibit the same cleavage efficiency on the Bacillus subtilis pre-chitosanase.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Geukens N,Parro V,Rivas LA,Mellado RP,Anné Jdoi
10.1007/s002030100335keywords:
subject
Has Abstractpub_date
2001-11-01 00:00:00pages
377-80issue
5eissn
0302-8933issn
1432-072Xjournal_volume
176pub_type
杂志文章abstract：:The role of HoxX in hydrogenase biosynthesis of Alcaligenes eutrophus H16 was re-examined. The previously characterized hoxX deletion mutant HF344 and a newly constructed second hoxX mutant carrying a smaller in-frame deletion were studied. The second mutant was impaired in the activity of both the soluble and the mem...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050667
更新日期：1998-11-01 00:00:00
abstract：:The set of physiological and metabolic changes occurring immediately after inoculation and during the lag phase is thought to be of vital importance for optimal offset of fermentation. The transcriptional changes taking place during the lag phase after inoculation of a late-respiratory-phase yeast culture into fresh, ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0527-6
更新日期：2003-04-01 00:00:00
abstract：:The induction by D,L-nicotine of the enantiozymes 6-hydroxy-L-nicotine oxidase and 6-hydroxy-D-nicotine oxidase in Arthrobacter oxidans was differently affected by the inhibitors of Escherichia coli gyrase, novobiocin and nalidixic acid. These compounds inhibited 6-hydroxy-L-nicotine oxidase induction slightly, but le...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00521289
更新日期：1982-12-03 00:00:00
abstract：:The twin-arginine translocation (Tat) system serves to export fully folded proteins across the cytoplasmic membrane. In many bacteria, three major components, TatA, TatB and TatC, are the functionally essential constituents of the Tat system. A Myxococcus xanthus tatB-tatC deletion mutant could aggregate and form moun...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0067-3
更新日期：2006-02-01 00:00:00
abstract：:Calcium is essential for the growth of rhizobia and the formation of nitrogen-fixing root-nodules on legumes, but its precise role in these processes remains unknown. We have found that Rhizobium fredii USDA208 accumulates a major 38 kDa protein when grown in media supplemented with 0.3-2 microM Cacl2. We have purifie...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00248480
更新日期：1993-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00427876
更新日期：1976-02-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0104-x
更新日期：2006-06-01 00:00:00
abstract：:Bacillus subtilis strain 168 encodes two flavocytochromes P450, Cyp102A2 and Cyp102A3. The cyp102A3 gene is preceded by, and organized in an operon with, a gene for a transcriptional regulator, encoded by fatR. The paralogous gene, cyp102A2, is most likely transcribed as a mono-cistronic message. We show that fatR enc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100350
更新日期：2001-12-01 00:00:00
abstract：:Two proteases, designated I and II, have been isolated from sporulating cells of Bacillus subtilis. They were partially purified by ammonium sulfate fractionation, Sephadex chromatography and affinity columns. Protease I was found to be similar to an already characterized B. subtilis protease. Protease II is trypsin-l...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425256
更新日期：1981-05-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00248837
更新日期：1990-01-01 00:00:00
abstract：:Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants virus was studied in Vero cells to determine maximum virus yield. One-step growth curve was formulated after determining virus in both supernatant (CFV) and cell lysate (CAV) at different time categories by microtitre plate titrat...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02154-w
更新日期：2021-01-05 00:00:00
abstract：:L-(+)-lactate dehydrogenase (LDH) from Staphylococcus epidermidis ATCC 14990 was purified by affinity chromatography. The purified enzyme was specifically activated by fructose-1,6-diphosphate (FDP). The concentration of FDP required for 50% maximal activity was about 0.15 mM. The enzyme activity was inhibited by aden...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447150
更新日期：1975-11-07 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01827-w
更新日期：2020-07-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00410896
更新日期：1984-08-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425066
更新日期：1979-06-01 00:00:00
abstract：:The endophytic diazotrophic Gluconacetobacter diazotrophicus PAL5 was originally isolated from sugarcane (Saccharum officinarum). The biological nitrogen fixation, phytohormones secretion, solubilization of mineral nutrients and phytopathogen antagonism allow its classification as a plant growth-promoting bacterium. T...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0794-1
更新日期：2012-07-01 00:00:00
abstract：:Bacteroides succinogenes produces acetate and succinate as major products of carbohydrate fermentation. An investigation of the enzymes involved indicated that pyruvate is oxidized by a flavin-dependent pyruvate cleavage enzyme to acetyl-CoA and CO2. Active CO2 exchange is associated with the pyruvate oxidation system...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00402302
更新日期：1978-05-30 00:00:00
abstract：:Exogenous addition of adenosine- and guanosine 5'-(di- and tri) phosphate 3'-diphosphate (pppApp, ppApp, pppGpp and ppGpp) at the concentration of 0.5 mM inhibits spore germination of Streptomyces galilaeus ATCC 31133. This reversible inhibitory effect appeared to be at the transcriptional level, and also depends on t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446889
更新日期：1980-04-01 00:00:00
abstract：:Pseudomonas putida CSV86 utilizes benzyl alcohol via catechol and methylnaphthalenes through detoxification pathway via hydroxymethylnaphthalenes and naphthaldehydes. Based on metabolic studies, benzyl alcohol dehydrogenase (BADH) and benzaldehyde dehydrogenase (BZDH) were hypothesized to be involved in the detoxifica...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0697-6
更新日期：2011-08-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01716-x
更新日期：2020-01-01 00:00:00
abstract：:We explored the molecular diversity of cytochrome P450 genes in the filamentous fungus Aspergillus oryzae using bioinformatic and experimental approaches. Based on bioinformatic annotation, we found 155 putative genes of cytochromes P450 in the whole genome sequence; however, 13 of 155 appeared to be pseudogenes due t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0562-z
更新日期：2010-05-01 00:00:00
abstract：:Milk containing antibiotics is used as cost-effective feed for calves, which may lead to antibiotic residues-associated food safety problems. This study aims to investigate the influence of antibiotics on rumen microbes. Through metagenomic sequencing, the rumen microbial communities of calves fed with pasteurized mil...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1311-8
更新日期：2017-04-01 00:00:00
abstract：:Although, there have been many published bacterial strains aerobically degrading the heterocyclic amine compounds, only one strain to date has been reported to degrade pyrrolidine under denitrifying conditions. In this study, denitrifying bacteria degrading pyrrolidine and piperidine were isolated from diverse geologi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0452-9
更新日期：2009-04-01 00:00:00
abstract：:Methanolic extract of Artemisia pallens (MEAP) (Asteraceae) was explored as greenbiocorrosion inhibitor for mild steel 1010 in 1.5% sodium chloride environment. Bacillus megaterium SKR7 induces the development of biofilm on the metal surface and forms the pitting corrosion. MEAP was showed (25 ppm) optimum inhibition ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01951-7
更新日期：2020-10-01 00:00:00
abstract：:The gliding bacterium Herpetosiphon giganteus Hp a2 was shown to contain gamma-carotene, one monoglucosyloxy and two new diglucosyloxy carotenoids with a x-O-acyldiglucosyloxy carotenoid as main component. Flexirubin-like pigments could not be detected in this organism. As in the Myxobacterales and Cytophagales (Klein...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413098
更新日期：1977-04-01 00:00:00
abstract：:Denitrification by Thiobacillus denitrificans "RT" strain was investigated using manometry and gas chromatography. 1. From nitrate, resting cells produced only nitrogen anaerobically with thiosulfate as the electron donor. The data suggest that nitrate was assimilated and dissimilated by the same nitrate reductase, as...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00436326
更新日期：1975-03-12 00:00:00
abstract：:A moderately psychrophilic purple non-sulfur bacterium, Rhodoferax antarcticus strain Fryx1, is described. Strain Fryx1 was isolated from the water column under the ice of the permanently frozen Lake Fryxell, Antarctica. Cells of Fryx1 are long thin rods and contain gas vesicles, the first report of such structures in...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0719-8
更新日期：2004-10-01 00:00:00
abstract：:The catabolic products of arginine metabolism were observed in Aphanocapsa 6308, a unicellular cyanobacterium, by thin layer chromatography of growth media, by limiting growth conditions, and by enzymatic analysis. Of the organic, nitrogenous compounds examined, only arginine supported growth in CO2-free media. The ex...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406066
更新日期：1978-07-01 00:00:00
abstract：:Thermotoga hypogea is an extremely thermophilic anaerobic bacterium capable of growing at 90 degrees C. It uses carbohydrates and peptides as carbon and energy sources to produce acetate, CO(2), H(2), L-alanine and ethanol as end products. Alcohol dehydrogenase activity was found to be present in the soluble fraction ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0217-x
更新日期：2007-06-01 00:00:00
abstract：:To address correlations between population sizes of microbes on the leaf surfaces and leaf morphological and physicochemical properties, various leaf morphological and physicochemical features as possible predictors of microbial population sizes on the leaf surfaces of four Napier grass cultivars were assessed. Result...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02025-4
更新日期：2021-01-01 00:00:00
