Regulation of the central metabolism in relation to citric acid production in Saccharomycopsis lipolytica.

Abstract:

:The mechanism of the massive extracellular production of citric and isocitric acids by Saccharomycopsis lipolytica grown on n-paraffins has been studied. When growth stops, because of nitrogen limitation, the intracellular concentration of ATP sharply rises whereas that of AMP and ADP decreases to a low level. At the same time production of acids begins. The activity of the NAD-dependent isocitrate dehydrogenase which requires AMP for activity becomes very low and prevents the oxidative function of the citric acid cycle whereas isocitrate lyase is not inhibited. As citrate synthase inhibition by ATP appears to be insufficient to stop n-paraffin degradation, citric and isocitric acids accumulation can take place. Massive excretion of these acids, however, probably still involves other physiological changes brought about by nitrogen limitation, possibly some permeabilization of the cell to these acids.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Marchal R,Vandecasteele JP,Metche M

doi

10.1007/BF00428588

subject

Has Abstract

pub_date

1977-05-13 00:00:00

pages

99-104

issue

1-2

eissn

0302-8933

issn

1432-072X

journal_volume

113

pub_type

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