Abstract：
:The tricarboxylic acid (TCA) cycle is one of the major routes of carbon catabolism in Bacillus subtilis. The syntheses of the enzymes performing the initial reactions of the cycle, citrate synthase, and aconitase, are synergistically repressed by rapidly metabolizable carbon sources and glutamine. This regulation involves the general transcription factor CcpA and the specific repressor CcpC. In this study, we analyzed the expression and intracellular localization of CcpC. The synthesis of citrate, the effector of CcpC, requires acetyl-CoA. This metabolite is located at a branching point in metabolism. It can be converted to acetate in overflow metabolism or to citrate. Manipulations of the fate of acetyl-CoA revealed that efficient citrate synthesis is required for the expression of the citB gene encoding aconitase and that control of the two pathways utilizing acetyl-CoA converges in the control of citrate synthesis for the induction of the TCA cycle. The citrate pool seems also to be controlled by arginine catabolism. The presence of arginine results in a severe CcpC-dependent repression of citB. In addition to regulators involved in sensing the carbon status of the cell, the pleiotropic nitrogen-related transcription factor, TnrA, activates citB transcription in the absence of glutamine.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Blencke HM,Reif I,Commichau FM,Detsch C,Wacker I,Ludwig H,Stülke Jdoi
10.1007/s00203-005-0078-0keywords:
subject
Has Abstractpub_date
2006-03-01 00:00:00pages
136-46issue
2eissn
0302-8933issn
1432-072Xjournal_volume
185pub_type
杂志文章abstract：:Cadmium is a non-essential toxic heavy metal for organisms, including plants and cyanobacteria. Cadmium resistance transporters involved in resistance of cells against various toxicants such as drugs and effluxes cytotoxic compounds from cells. However, cadmium resistance-associated protein (CadD) has never been repor...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1462-2
更新日期：2018-04-01 00:00:00
abstract：:In Lactobacillus casei BL23, phosphofructokinase activity was higher in cells utilizing sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system (PTS). The phosphofructokinase gene (pfk) was cloned from L. casei and shown to be clustered with the gene encoding pyruvate kinase (pyk). pfk and pyk ge...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0003-6
更新日期：2005-09-01 00:00:00
abstract：:Maize is highly responsive to the application of nitrogen to achieve high productivity. Inoculation with diazotrophic bacteria can improve plant growth with low N fertilization. The objective was to evaluate the inoculation of two species of diazotrophs on N metabolism in maize plants, in the presence of two concentra...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-018-1594-z
更新日期：2019-05-01 00:00:00
abstract：:The effect of intestinal molecules produced by the host on the virulence of Pseudomonas fluorescens is poorly documented. In the present work, we evaluated the secretion of human β-defensin-2 (hBD-2) by enterocytes after infection with P. fluorescens (a species previously suggested to be involved in inflammatory bowel...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0865-3
更新日期：2013-03-01 00:00:00
abstract：:Previous work has shown that the mechanosensitive (MS) channel of large conductance (MscL) is essential for preventing lysis of Bacillus subtilis log phase cells upon a rapid, severe osmotic downshock. Growing cells of B. subtilis strains lacking MscL and one or more putative MS channel proteins of small conductance (...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0292-z
更新日期：2008-01-01 00:00:00
abstract：:Bacterial pathogens are a major threat to both humans and animals worldwide. It is crucial to understand the mechanisms of various disease processes at the molecular level. Shewanella species are widespread in the environment and some are considered as emerging opportunistic human and marine mammal pathogens. In this ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02060-1
更新日期：2020-10-10 00:00:00
abstract：:Glucose-limited continuous cultures were used to analyze sigmaB activity at decreasing growth rates. Expression of the sigmaB-dependent genes gsiB and ctc started to increase at a growth rate of 0.2 h-1, and both genes were induced approximately fivefold at a growth rate of 0.1 h-1 as compared to expression at the max...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050731
更新日期：1999-05-01 00:00:00
abstract：:Nitrosomonas europaea, a Gram-negative obligate chemolithoautotroph, participates in global nitrogen cycling by carrying out nitrification and derives energy for growth through oxidation of ammonia. In this work, the physiological, proteomic, and transcriptional responses of N. europaea to zinc stress were studied. Th...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0341-7
更新日期：2008-06-01 00:00:00
abstract：:The endophytic diazotrophic Gluconacetobacter diazotrophicus PAL5 was originally isolated from sugarcane (Saccharum officinarum). The biological nitrogen fixation, phytohormones secretion, solubilization of mineral nutrients and phytopathogen antagonism allow its classification as a plant growth-promoting bacterium. T...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0794-1
更新日期：2012-07-01 00:00:00
abstract：:We cloned the Kluyveromyces lactis KlNTH1 gene, which encodes neutral trehalase. It showed 65.2% and 68.5% identity at nucleotide and amino acid sequence level, respectively, with the Saccharomyces cerevisiae NTH1 gene. Multiple alignment of the predicted trehalase protein sequences from yeasts, bacteria, insects, and...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050436
更新日期：1997-04-01 00:00:00
abstract：:The L-alanine mediated germination of food isolated Bacillus cereus DSA 1 spores, which lacked an intact exosporium, increased in the presence of D-cycloserine (DCS), which is an alanine racemase (Alr) inhibitor, reflecting the activity of the Alr enzyme, capable of converting L-alanine to the germination inhibitor D-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0946-y
更新日期：2014-02-01 00:00:00
abstract：:Synechococcus elongatus strain PCC7942 cells were grown in high or low environmental concentrations of inorganic C (high-C(i), low-C(i)) and subjected to a light shift from 50 micromol m(-2) s(-1) to 500 micromol m(-2) s(-1). We quantified photosynthetic reductant (O(2) evolution) and molar cellular contents of phycob...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0761-1
更新日期：2005-03-01 00:00:00
abstract：:In autotrophic methanogens, pyruvate oxidoreductase (POR) plays a key role in the assimilation of CO(2) and the biosynthesis of organic carbon. This enzyme has been purified to homogeneity, and the genes from Methanococcus maripaludis were sequenced. The purified POR contained five polypeptides with molecular masses o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0554-3
更新日期：2003-06-01 00:00:00
abstract：:Microbial changes in vaginal ecosystem may accelerate the process of cervical carcinogenesis. The developed cervical cancer can lead to changes in the vaginal microbiota. The aim of our study is to determine the vaginal microbiota changes at women with FIGO I stage cervical cancer. We conducted an open, single-site su...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01747-4
更新日期：2020-03-01 00:00:00
abstract：:The ontogeny of the myxomycete Physarum globuliferum was observed on corn meal agar and hanging drop cultures without adding sterile oat flakes, bacteria or other microorganisms. Its complete life cycle including spore germination, myxamoebae, swarm cells, plasmodial development, and maturity of fructifications was de...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0533-4
更新日期：2010-02-01 00:00:00
abstract：:The present study investigated the endophytic bacterial communities in the seeds of mature, natural common reed (Phragmites australis) and narrowleaf cattail (Typha angustifolia L.). Additionally, seed endophytic bacterial communities were compared with rhizospheric and root endophytic bacterial communities using Illu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-018-1493-3
更新日期：2018-08-01 00:00:00
abstract：:Chemoautotrophic endosymbionts residing in Solemya velum gills provide this shallow water clam with most of its nutritional requirements. The cbb gene cluster of the S. velum symbiont, including cbbL and cbbS, which encode the large and small subunits of the carbon-fixing enzyme ribulose 1,5-bisphosphate carboxylase/o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0689-x
更新日期：2004-09-01 00:00:00
abstract：:An Arthrobacter sp. growing on 4-Chlorobenzoic acid as its sole source of carbon excretes 4-hydroxygenzoic acid and protocatechuic acid into the culture medium. Protocatechuic acid is further attacked by "meta"-cleavage. During growth of the Arthrobacter sp. on benzoic acid cis-cis muconic acid can be isolated from th...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00690235
更新日期：1976-11-02 00:00:00
abstract：:The induction by D,L-nicotine of the enantiozymes 6-hydroxy-L-nicotine oxidase and 6-hydroxy-D-nicotine oxidase in Arthrobacter oxidans was differently affected by the inhibitors of Escherichia coli gyrase, novobiocin and nalidixic acid. These compounds inhibited 6-hydroxy-L-nicotine oxidase induction slightly, but le...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00521289
更新日期：1982-12-03 00:00:00
abstract：:The distribution of sex pheromone induced aggregation substance was studied on the cell surface of various Enterococcus faecalis strains. In the accompanying paper we have shown that the aggregation substance appears as a layer of hairlike structures. Using direct and indirect immunogold technique, transmission electr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454864
更新日期：1989-01-01 00:00:00
abstract：:The presence of teichoic acids in a number of streptomycetes led to the conclusion that these biopolymers were widely spread in genus Streptomyces. The nature of the teichoic acid present in the mycelium was determined by extracting it with 10% trichloroacetic acid, precipitating it with ethanol and identifying the pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00421893
更新日期：1980-05-01 00:00:00
abstract：:In this study, chromium (Cr)-tolerant bacteria were test for their efficiency in alleviating Cr stress in Cicer arietinum plants. On the basis of 16S rRNA gene analysis, the isolates were identified belonging to genus Stenotrophomonas maltophilia, Bacillus thuringiensis B. cereus, and B. subtilis. The strains produced...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01801-1
更新日期：2020-05-01 00:00:00
abstract：:Monoclonal antibodies have been used to determine the presence of cellobiohydrolases I and II (CBH I and II), and endoglucanase I (EG I) on the surface of conidia from Trichoderma reesei QM 9414 and RUT C-30, and 8 other Trichoderma species. For this purpose, proteins were released from the conidial surface by treatme...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245356
更新日期：1991-01-01 00:00:00
abstract：:A neutral protease (npr) (designated Bae16) toxic to nematodes was purified to homogeneity from the strain Bacillus nematocida. The purified protease showed a molecular mass of approximately 40 kDa and displayed optimal activity at 55 degrees C, pH 6.5. Bioassay experiments demonstrated that this purified protease cou...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0112-x
更新日期：2006-06-01 00:00:00
abstract：:The bacteriocinogenic strain of Enterococcus faecalis EJ97 has been isolated from municipal waste water. It produces a cationic bacteriocin (enterocin EJ97) of low molecular mass (5,340 Da) that is very stable under mild heat conditions and is sensitive to proteolytic enzymes. The amino acid sequence of the first 18 N...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050678
更新日期：1998-12-01 00:00:00
abstract：:An alkaliphilic actinobacterial strain, designated Hz 6-5T, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China. The isolate formed light yellow-colored colonies and its cells were Gram-staining positive, non-motile, and non-spore-forming short rods. The strain was aerobic with optimal gro...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02073-w
更新日期：2020-10-26 00:00:00
abstract：:During the development of fruit bodies of the basidiomycete Schizophyllum commune, the alkali-insoluble (R glucan) and alkali-soluble (S glucan) cell wall fractions are synthesized during the entire course of morphogenesis. The water soluble glucan (WSG) is not synthesized after an early stage. There is also a relativ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429623
更新日期：1977-07-26 00:00:00
abstract：:In this study, on evaluating the physiological function and mechanism of putrescine, we found that putrescine supplementation (1 mM) increases transcription of the putrescine aminotransferase gene, ygjG. Putrescine-dependent expression was confirmed by measuring β-galactosidase activity and with reverse transcription-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-0991-1
更新日期：2014-09-01 00:00:00
abstract：:A highly efficient method of transposon mutagenesis was developed for genetic analysis of Xanthobacter autotrophicus Py2. The method makes use of a transposon delivery vector that encodes a hyperactive Tn 5 transposase that is 1,000-fold more active than the wild-type transposase. In this construct, the transposase is...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0442-2
更新日期：2002-09-01 00:00:00
abstract：:The hmc operon of Desulfovibrio vulgaris subsp. vulgaris Hildenborough consists of six genes (hmcA to hmcF) that encode structural components of the high-molecular-mass cytochrome redox protein complex (the Hmc complex). Two genes (rrf1 and rrf2) encoding regulatory proteins are present downstream of hmcF. Expression ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050458
更新日期：1997-06-01 00:00:00
