Abstract:
:Previous work has shown that the mechanosensitive (MS) channel of large conductance (MscL) is essential for preventing lysis of Bacillus subtilis log phase cells upon a rapid, severe osmotic downshock. Growing cells of B. subtilis strains lacking MscL and one or more putative MS channel proteins of small conductance (YhdY, YkuT and YfkC) showed even higher sensitivity to an osmotic downshock. The effect was greatest for a strain lacking MscL and YkuT, and a strain lacking all four MS channel proteins had a similar phenotype. These defects were complemented by expression of either MscL or YkuT in trans. All MS channel mutant strains ultimately became resistant to osmotic downshock in stationary phase but at varying times, with mscL ykuT strains taking the longest time to become resistant. Expression of beta-galactosidase from gene fusions to lacZ showed modest expression of ykuT and lower levels of expression of yhdY and yfkC when strains were grown in medium containing high salt. Sporulation of all MS channel mutant strains was normal, and the mutant spores germinated normally with L-alanine or dodecylamine.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Wahome PG,Setlow Pdoi
10.1007/s00203-007-0292-zsubject
Has Abstractpub_date
2008-01-01 00:00:00pages
49-58issue
1eissn
0302-8933issn
1432-072Xjournal_volume
189pub_type
杂志文章abstract::It has been shown previously that externally added glycine betaine is accumulated in Escherichia coli in response to the external osmotic strength. Here we have shown, by using nuclear magnetic resonance spectroscopy and radiochemical methods, that E. coli growing in a glucose-mineral medium of elevated osmotic streng...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00492896
更新日期:1987-02-01 00:00:00
abstract::Double immunodiffusion tests were performed with crude extracts from various Micrococcus species and antisera against catalase of Micrococcus luteus CCM 169. Cell-free extracts of M. lylae ATCC 27566 exhibited good cross-reaction. Cell-free extracts or catalase enriched preparations of M. varians reacted very weakly a...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00429631
更新日期:1977-07-26 00:00:00
abstract::Clostridium butyricum is widely used to produce organic solvents such as ethanol, butanol and acetone. We sequenced the entire genome of C. butyricum INCQS635 by using Ion Torrent technology. We found a high contribution of sequences assigned for carbohydrate subsystems (15-20 % of known sequences). Annotation based o...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-015-1166-4
更新日期:2016-03-01 00:00:00
abstract::In order to obtain strains of Saccharomycopsis lipolytica impaired in the active transport of L-lysine, mutants resistant to a mixture of L-canavanine, L-4-5-transdehydrolysine and L-S-amino ethylcysteine, taken either all three or two by two, were isolated. These compounds were shown previously to be competitive inhi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00411361
更新日期:1979-08-06 00:00:00
abstract::The morphogenesis of plasmodium in Didymium megalosporum was observed for the first time by hanging drop culture and 3 % oat-agar culture under controlled conditions. The development of plasmodium was characteristic formation process of phaneroplasmodium. The mature plasmodium was white yellow or yellow green in color...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-0971-5
更新日期:2014-05-01 00:00:00
abstract::In view of the functional similarities between subtilisin Carlsberg and the alkaline protease from Conidiobolus coronatus, the biochemical and structural properties of the two enzymes were compared. In spite of their similar biochemical properties, e.g., pH optima, heat stability, molecular mass, pI, esterase activity...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF01682989
更新日期:1996-12-01 00:00:00
abstract::Acinetobacter strain IVS-B aerobically grows on isovalerate as sole carbon and energy source. Isovalerate is metabolised via isovaleryl-CoA, an intermediate of the oxidative (S)-leucine degradation pathway. A 3-methylglutaconyl-CoA hydratase (EC 4.2.1.18) was purified 65-fold to apparent homogeneity from cell-free ext...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-006-0095-7
更新日期:2006-05-01 00:00:00
abstract::Polysomes were isolated both from growing gametophytes of Allomyces arbuscula and from gametangia prepared from mycelia at different periods during gametogenesis. Analysis of polysomes by sucrose gradients showed that ribosomes present in the gametangia monosome pool were shifted into polysomes. This shift was found t...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00413089
更新日期:1977-04-01 00:00:00
abstract::During the course of isolating rare actinobacteria from unexplored habitats, strain CH32T was obtained from an arid soil sample in eastern Anatolia, Turkey. Polyphasic characterization and comprehensive genome analyses showed that the strain is a member of the genus Nonomuraea and it is closely related to Nonomuraea g...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01941-9
更新日期:2020-10-01 00:00:00
abstract::For the low molecular weight laccases II and III of Podospora anserina the kinetic parameters Michaelis constant (KM) and maximum reaction velocity (V) were determined polarographically under pH optimum conditions for representative substrates of different substitution patterns. Laccase II showed two peaks in its pH o...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446663
更新日期:1977-02-04 00:00:00
abstract::In order to investigate the role of the putative epimerase function of the beta-oxidation multienzyme complex (FadBA) in the provision of (R)-3-hydroxyacyl-CoA thioesters for medium-chain-length polyhydroxyalkanoate (PHA(MCL)) biosynthesis, the fadBA(Po) operon of Pseudomonas oleovorans was cloned and characterized. T...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0444-0
更新日期:2002-08-01 00:00:00
abstract::The aim of this study was to investigate the viable but nonculturable (VBNC) state of the bacterium. Vibrio alginolyticus VIB283 was cultured in sterilized seawater microcosm at 4 degrees C. Culturability of the cells in the microcosm was monitored by spread plate count (PC) on 2216E agar, PCs declined to undetectable...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0246-5
更新日期:2007-09-01 00:00:00
abstract::Gas chromatography-mass spectrometry studies of the nitrogen isotopic composition of the N-trifluoroacetyl n-butyl ester derivatives of the amino acids from isolated hydrolyzed cyanophycin from 15N-enriched cells led to two major findings: (1) the amino acid composition of this granular polypeptide, isolated using pro...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00314469
更新日期:1994-01-01 00:00:00
abstract::Effects of tunicamycin (TM) on the sexual agglutinability and zygote formation of Saccharomyces cerevisiae were studied using the two kinds of haploid strains, inducible and constitutive for sexual agglutinability. Induction of sexual agglutinability by opposite mating type sex pheromone of inducible strains was inhib...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00410896
更新日期:1984-08-01 00:00:00
abstract::The marine bacterium Vibrio psychoerythrus, an obligate psychrophile, contains uniformly dense rod-shaped organelles 10-15 nm wide and up to 1.3 mum long. These structures were frequently seen crossing the septum of dividing cells. ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00427869
更新日期:1976-02-01 00:00:00
abstract::DNA sequence analysis of the structural urease genes from Staphylococcus xylosus revealed that three enzyme subunits are encoded in the order of 11,000, 15,400 and 61,000 (mol. mass), which correspond to the single polypeptide chain of jack bean urease (90,800). Comparing the deduced amino acid sequence of S. xylosus ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00288947
更新日期:1994-01-01 00:00:00
abstract::Calcium is essential for the growth of rhizobia and the formation of nitrogen-fixing root-nodules on legumes, but its precise role in these processes remains unknown. We have found that Rhizobium fredii USDA208 accumulates a major 38 kDa protein when grown in media supplemented with 0.3-2 microM Cacl2. We have purifie...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00248480
更新日期:1993-01-01 00:00:00
abstract::The induction of the enzyme activities involved in butane metabolism in Pseudomonas butanovora was characterized. P. butanovora was grown on butane or its metabolites, both singly and in mixtures with other growth substrates. Cells grown in each of the butane metabolites readily consumed the growth substrate and downs...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030100304
更新日期:2001-07-01 00:00:00
abstract::Halophilic archaeal strain ZS-47-ST was isolated from Zhoushan marine solar saltern, China. Cells were pleomorphic, stained Gram-negative, and formed red-pigmented colonies on agar plate. Strain ZS-47-ST was able to grow at 20-50 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 3.4 M), at 0.005-1.0 M MgCl2 (optimum 0.03...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-017-1420-z
更新日期:2017-12-01 00:00:00
abstract::A highly efficient method of transposon mutagenesis was developed for genetic analysis of Xanthobacter autotrophicus Py2. The method makes use of a transposon delivery vector that encodes a hyperactive Tn 5 transposase that is 1,000-fold more active than the wild-type transposase. In this construct, the transposase is...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0442-2
更新日期:2002-09-01 00:00:00
abstract::An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 degrees C and 5.4 g NaCl l(-1). The strain grows heterotrophically on complex pr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050745
更新日期:1999-08-01 00:00:00
abstract::Only about one third of humans possess a microbiota capable of transforming the dietary isoflavone daidzein into equol. Little is known about the dietary and physiological factors determining this ecological feature. In this study, the in vitro metabolism of daidzein by faecal samples from four human individuals was i...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0747-4
更新日期:2005-01-01 00:00:00
abstract::The hmc operon of Desulfovibrio vulgaris subsp. vulgaris Hildenborough consists of six genes (hmcA to hmcF) that encode structural components of the high-molecular-mass cytochrome redox protein complex (the Hmc complex). Two genes (rrf1 and rrf2) encoding regulatory proteins are present downstream of hmcF. Expression ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050458
更新日期:1997-06-01 00:00:00
abstract::Enzymes that are regulated by the ferredoxin/thioredoxin system in chloroplasts--fructose-1,6-bis-phosphatase (FBPase), sedoheptulose-1,7-bisphosphatase (SBPase), and phosphoribulokinase (PRK)--were partially purified from two different types of photosynthetic prokaryotes (cyanobacteria, purple sulfur bacteria) and te...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00401986
更新日期:1984-10-01 00:00:00
abstract::Twelve Cry1 and two Cry9 delta-endotoxins from Bacillus thuringiensis were tested for their activity against black cutworm ( Agrotis ipsilon). A. ipsilon was not susceptible to many toxins, but three toxins had significant activity. Cry9Ca was the most toxic, followed by Cry1Aa and Cry1Fb. Hybrids between these three ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0543-6
更新日期:2003-05-01 00:00:00
abstract::Listeria monocytogenes was examined for the presence of surface carbohydrates to ascertain whether surface sugars, if present, would interact with eucaryotic surface carbohydrate receptors. We found that a virulent, but not two avirulent strains had a surface alpha-D-galactose residue as determined by agglutination wi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00249083
更新日期:1990-01-01 00:00:00
abstract::The proteins in the culture supernatant (exoproteins) from Streptococcus pyogenes serotype M1 were separated by two-dimensional gel electrophoresis, and their N-terminal amino acid sequences were determined. The amino acid sequences were compared to sequences in the S. pyogenes genome database. The coding sequence sho...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0412-8
更新日期:2002-06-01 00:00:00
abstract::Fifty isolates belonging to the six validly described species of the genus Thermus (T. aquaticus, T. filiformis, T. thermophilus, T. scotoductus, T. brockianus, and T. oshimai) isolated from hot springs of different geographical areas were compared using macrorestriction analysis of genomic DNA and ribotyping. With th...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050474
更新日期:1997-08-01 00:00:00
abstract::Tetrahydrofolate was shown to function as a methyl acceptor in the anaerobic demethylation of dimethylsulfoniopropionate to methylthiopropionate in cell extracts of the sulfate-reducing bacterium strain WN. Dimethylsulfoniopropionate-dependent activities were 0.56 micromol methyltetrahydrofolate min-1 (mg protein)-1 a...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050545
更新日期:1998-01-01 00:00:00
abstract::Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach. The optimum growth temperature was 37 °C and the optimum pH was 7.5-...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02003-w
更新日期:2021-01-01 00:00:00