Growth, osmotic downshock resistance and differentiation of Bacillus subtilis strains lacking mechanosensitive channels.

Abstract:

:Previous work has shown that the mechanosensitive (MS) channel of large conductance (MscL) is essential for preventing lysis of Bacillus subtilis log phase cells upon a rapid, severe osmotic downshock. Growing cells of B. subtilis strains lacking MscL and one or more putative MS channel proteins of small conductance (YhdY, YkuT and YfkC) showed even higher sensitivity to an osmotic downshock. The effect was greatest for a strain lacking MscL and YkuT, and a strain lacking all four MS channel proteins had a similar phenotype. These defects were complemented by expression of either MscL or YkuT in trans. All MS channel mutant strains ultimately became resistant to osmotic downshock in stationary phase but at varying times, with mscL ykuT strains taking the longest time to become resistant. Expression of beta-galactosidase from gene fusions to lacZ showed modest expression of ykuT and lower levels of expression of yhdY and yfkC when strains were grown in medium containing high salt. Sporulation of all MS channel mutant strains was normal, and the mutant spores germinated normally with L-alanine or dodecylamine.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Wahome PG,Setlow P

doi

10.1007/s00203-007-0292-z

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

49-58

issue

1

eissn

0302-8933

issn

1432-072X

journal_volume

189

pub_type

杂志文章
  • Osmoregulation in Escherichia coli by accumulation of organic osmolytes: betaines, glutamic acid, and trehalose.

    abstract::It has been shown previously that externally added glycine betaine is accumulated in Escherichia coli in response to the external osmotic strength. Here we have shown, by using nuclear magnetic resonance spectroscopy and radiochemical methods, that E. coli growing in a glucose-mineral medium of elevated osmotic streng...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00492896

    authors: Larsen PI,Sydnes LK,Landfald B,Strøm AR

    更新日期:1987-02-01 00:00:00

  • Comparative immunological study of catalases in the genus Micrococcus.

    abstract::Double immunodiffusion tests were performed with crude extracts from various Micrococcus species and antisera against catalase of Micrococcus luteus CCM 169. Cell-free extracts of M. lylae ATCC 27566 exhibited good cross-reaction. Cell-free extracts or catalase enriched preparations of M. varians reacted very weakly a...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00429631

    authors: Rupprecht M,Schleifer KH

    更新日期:1977-07-26 00:00:00

  • Insights from genome of Clostridium butyricum INCQS635 reveal mechanisms to convert complex sugars for biofuel production.

    abstract::Clostridium butyricum is widely used to produce organic solvents such as ethanol, butanol and acetone. We sequenced the entire genome of C. butyricum INCQS635 by using Ion Torrent technology. We found a high contribution of sequences assigned for carbohydrate subsystems (15-20 % of known sequences). Annotation based o...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-015-1166-4

    authors: Bruce T,Leite FG,Miranda M,Thompson CC,Pereira N Jr,Faber M,Thompson FL

    更新日期:2016-03-01 00:00:00

  • Genetic control of lysine permeases in Saccharomycopsis lipolytica.

    abstract::In order to obtain strains of Saccharomycopsis lipolytica impaired in the active transport of L-lysine, mutants resistant to a mixture of L-canavanine, L-4-5-transdehydrolysine and L-S-amino ethylcysteine, taken either all three or two by two, were isolated. These compounds were shown previously to be competitive inhi...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00411361

    authors: Beckerich JM,Lambert M,Heslot H

    更新日期:1979-08-06 00:00:00

  • Morphogenesis and alpha-tubulin gene of plasmodium in Didymium megalosporum.

    abstract::The morphogenesis of plasmodium in Didymium megalosporum was observed for the first time by hanging drop culture and 3 % oat-agar culture under controlled conditions. The development of plasmodium was characteristic formation process of phaneroplasmodium. The mature plasmodium was white yellow or yellow green in color...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-014-0971-5

    authors: Song X,Zhong H,Wang Q,Li Y

    更新日期:2014-05-01 00:00:00

  • A serine alkaline protease from the fungus Conidiobolus coronatus with a distinctly different structure than the serine protease subtilisin Carlsberg.

    abstract::In view of the functional similarities between subtilisin Carlsberg and the alkaline protease from Conidiobolus coronatus, the biochemical and structural properties of the two enzymes were compared. In spite of their similar biochemical properties, e.g., pH optima, heat stability, molecular mass, pI, esterase activity...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF01682989

    authors: Phadtare S,Rao M,Deshpande V

    更新日期:1996-12-01 00:00:00

  • 3-Methylglutaconyl-CoA hydratase from Acinetobacter sp.

    abstract::Acinetobacter strain IVS-B aerobically grows on isovalerate as sole carbon and energy source. Isovalerate is metabolised via isovaleryl-CoA, an intermediate of the oxidative (S)-leucine degradation pathway. A 3-methylglutaconyl-CoA hydratase (EC 4.2.1.18) was purified 65-fold to apparent homogeneity from cell-free ext...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-006-0095-7

    authors: Mack M,Liesert M,Zschocke J,Peters V,Linder D,Buckel W

    更新日期:2006-05-01 00:00:00

  • [Studies on development of the phycomycete Allomyces arbuscula. III. Polysome formation and RNA metabolism during differentiation of gametangia (author's transl)].

    abstract::Polysomes were isolated both from growing gametophytes of Allomyces arbuscula and from gametangia prepared from mycelia at different periods during gametogenesis. Analysis of polysomes by sucrose gradients showed that ribosomes present in the gametangia monosome pool were shifted into polysomes. This shift was found t...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00413089

    authors: Fähnrich P

    更新日期:1977-04-01 00:00:00

  • Nonomuraea terrae sp. nov., isolated from arid soil.

    abstract::During the course of isolating rare actinobacteria from unexplored habitats, strain CH32T was obtained from an arid soil sample in eastern Anatolia, Turkey. Polyphasic characterization and comprehensive genome analyses showed that the strain is a member of the genus Nonomuraea and it is closely related to Nonomuraea g...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-020-01941-9

    authors: Ay H

    更新日期:2020-10-01 00:00:00

  • The phenol oxidases of the ascomycete Podospora anserina. XII. Affinity of laccases II and III to substrates with different substitution patterns.

    abstract::For the low molecular weight laccases II and III of Podospora anserina the kinetic parameters Michaelis constant (KM) and maximum reaction velocity (V) were determined polarographically under pH optimum conditions for representative substrates of different substitution patterns. Laccase II showed two peaks in its pH o...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00446663

    authors: Hoffmann P,Esser K

    更新日期:1977-02-04 00:00:00

  • The role of the fatty acid beta-oxidation multienzyme complex from Pseudomonas oleovorans in polyhydroxyalkanoate biosynthesis: molecular characterization of the fadBA operon from P. oleovorans and of the enoyl-CoA hydratase genes phaJ from P. oleovorans

    abstract::In order to investigate the role of the putative epimerase function of the beta-oxidation multienzyme complex (FadBA) in the provision of (R)-3-hydroxyacyl-CoA thioesters for medium-chain-length polyhydroxyalkanoate (PHA(MCL)) biosynthesis, the fadBA(Po) operon of Pseudomonas oleovorans was cloned and characterized. T...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-002-0444-0

    authors: Fiedler S,Steinbüchel A,Rehm BH

    更新日期:2002-08-01 00:00:00

  • Characterization and resuscitation of viable but nonculturable Vibrio alginolyticus VIB283.

    abstract::The aim of this study was to investigate the viable but nonculturable (VBNC) state of the bacterium. Vibrio alginolyticus VIB283 was cultured in sterilized seawater microcosm at 4 degrees C. Culturability of the cells in the microcosm was monitored by spread plate count (PC) on 2216E agar, PCs declined to undetectable...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-007-0246-5

    authors: Du M,Chen J,Zhang X,Li A,Li Y

    更新日期:2007-09-01 00:00:00

  • Variations in the amino acid composition of cyanophycin in the cyanobacterium Synechocystis sp. PCC 6308 as a function of growth conditions.

    abstract::Gas chromatography-mass spectrometry studies of the nitrogen isotopic composition of the N-trifluoroacetyl n-butyl ester derivatives of the amino acids from isolated hydrolyzed cyanophycin from 15N-enriched cells led to two major findings: (1) the amino acid composition of this granular polypeptide, isolated using pro...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00314469

    authors: Merritt MV,Sid SS,Mesh L,Allen MM

    更新日期:1994-01-01 00:00:00

  • Alpha-mating-type-specific suppression and a-mating-type-specific enhancement by tunicamycin of sexual agglutinability in the yeast Saccharomyces cerevisiae.

    abstract::Effects of tunicamycin (TM) on the sexual agglutinability and zygote formation of Saccharomyces cerevisiae were studied using the two kinds of haploid strains, inducible and constitutive for sexual agglutinability. Induction of sexual agglutinability by opposite mating type sex pheromone of inducible strains was inhib...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00410896

    authors: Hasegawa S,Yanagishima N

    更新日期:1984-08-01 00:00:00

  • Tubular structures of Vibrio psychroerythrus.

    abstract::The marine bacterium Vibrio psychoerythrus, an obligate psychrophile, contains uniformly dense rod-shaped organelles 10-15 nm wide and up to 1.3 mum long. These structures were frequently seen crossing the septum of dividing cells. ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00427869

    authors: D'Aoust JY,Kushner DJ

    更新日期:1976-02-01 00:00:00

  • Threonine is present instead of cysteine at the active site of urease from Staphylococcus xylosus.

    abstract::DNA sequence analysis of the structural urease genes from Staphylococcus xylosus revealed that three enzyme subunits are encoded in the order of 11,000, 15,400 and 61,000 (mol. mass), which correspond to the single polypeptide chain of jack bean urease (90,800). Comparing the deduced amino acid sequence of S. xylosus ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00288947

    authors: Jose J,Schäfer UK,Kaltwasser H

    更新日期:1994-01-01 00:00:00

  • Purification, partial characterization, and subcellular localization of a 38 kilodalton, calcium-regulated protein of Rhizobium fredii USDA208.

    abstract::Calcium is essential for the growth of rhizobia and the formation of nitrogen-fixing root-nodules on legumes, but its precise role in these processes remains unknown. We have found that Rhizobium fredii USDA208 accumulates a major 38 kDa protein when grown in media supplemented with 0.3-2 microM Cacl2. We have purifie...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00248480

    authors: Krishman HB,Pueppke SG

    更新日期:1993-01-01 00:00:00

  • Induction of butane consumption in Pseudomonas butanovora.

    abstract::The induction of the enzyme activities involved in butane metabolism in Pseudomonas butanovora was characterized. P. butanovora was grown on butane or its metabolites, both singly and in mixtures with other growth substrates. Cells grown in each of the butane metabolites readily consumed the growth substrate and downs...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030100304

    authors: Sayavedra-Soto LA,Byrd CM,Arp DJ

    更新日期:2001-07-01 00:00:00

  • Halomarina rubra sp. nov., isolated from a marine solar saltern.

    abstract::Halophilic archaeal strain ZS-47-ST was isolated from Zhoushan marine solar saltern, China. Cells were pleomorphic, stained Gram-negative, and formed red-pigmented colonies on agar plate. Strain ZS-47-ST was able to grow at 20-50 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 3.4 M), at 0.005-1.0 M MgCl2 (optimum 0.03...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-017-1420-z

    authors: Zhou Y,Li Y,Lü ZZ,Cui HL

    更新日期:2017-12-01 00:00:00

  • Genetic analysis of pigment biosynthesis in Xanthobacter autotrophicus Py2 using a new, highly efficient transposon mutagenesis system that is functional in a wide variety of bacteria.

    abstract::A highly efficient method of transposon mutagenesis was developed for genetic analysis of Xanthobacter autotrophicus Py2. The method makes use of a transposon delivery vector that encodes a hyperactive Tn 5 transposase that is 1,000-fold more active than the wild-type transposase. In this construct, the transposase is...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-002-0442-2

    authors: Larsen RA,Wilson MM,Guss AM,Metcalf WW

    更新日期:2002-09-01 00:00:00

  • Thermococcus waiotapuensis sp. nov., an extremely thermophilic archaeon isolated from a freshwater hot spring.

    abstract::An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 degrees C and 5.4 g NaCl l(-1). The strain grows heterotrophically on complex pr...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030050745

    authors: González JM,Sheckells D,Viebahn M,Krupatkina D,Borges KM,Robb FT

    更新日期:1999-08-01 00:00:00

  • Isolation and characterisation of an equol-producing mixed microbial culture from a human faecal sample and its activity under gastrointestinal conditions.

    abstract::Only about one third of humans possess a microbiota capable of transforming the dietary isoflavone daidzein into equol. Little is known about the dietary and physiological factors determining this ecological feature. In this study, the in vitro metabolism of daidzein by faecal samples from four human individuals was i...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-004-0747-4

    authors: Decroos K,Vanhemmens S,Cattoir S,Boon N,Verstraete W

    更新日期:2005-01-01 00:00:00

  • Deletion of two downstream genes alters expression of the hmc operon of Desulfovibrio vulgaris subsp. vulgaris Hildenborough.

    abstract::The hmc operon of Desulfovibrio vulgaris subsp. vulgaris Hildenborough consists of six genes (hmcA to hmcF) that encode structural components of the high-molecular-mass cytochrome redox protein complex (the Hmc complex). Two genes (rrf1 and rrf2) encoding regulatory proteins are present downstream of hmcF. Expression ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030050458

    authors: Keon RG,Fu R,Voordouw G

    更新日期:1997-06-01 00:00:00

  • Contrasting modes of photosynthetic enzyme regulation in oxygenic and anoxygenic prokaryotes.

    abstract::Enzymes that are regulated by the ferredoxin/thioredoxin system in chloroplasts--fructose-1,6-bis-phosphatase (FBPase), sedoheptulose-1,7-bisphosphatase (SBPase), and phosphoribulokinase (PRK)--were partially purified from two different types of photosynthetic prokaryotes (cyanobacteria, purple sulfur bacteria) and te...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00401986

    authors: Crawford NA,Sutton CW,Yee BC,Johnson TC,Carlson DC,Buchanan BB

    更新日期:1984-10-01 00:00:00

  • Activity of wild-type and hybrid Bacillus thuringiensis delta-endotoxins against Agrotis ipsilon.

    abstract::Twelve Cry1 and two Cry9 delta-endotoxins from Bacillus thuringiensis were tested for their activity against black cutworm ( Agrotis ipsilon). A. ipsilon was not susceptible to many toxins, but three toxins had significant activity. Cry9Ca was the most toxic, followed by Cry1Aa and Cry1Fb. Hybrids between these three ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-003-0543-6

    authors: de Maagd RA,Weemen-Hendriks M,Molthoff JW,Naimov S

    更新日期:2003-05-01 00:00:00

  • Adherence of a virulent strain of Listeria monocytogenes to the surface of a hepatocarcinoma cell line via lectin-substrate interaction.

    abstract::Listeria monocytogenes was examined for the presence of surface carbohydrates to ascertain whether surface sugars, if present, would interact with eucaryotic surface carbohydrate receptors. We found that a virulent, but not two avirulent strains had a surface alpha-D-galactose residue as determined by agglutination wi...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00249083

    authors: Cowart RE,Lashmet J,McIntosh ME,Adams TJ

    更新日期:1990-01-01 00:00:00

  • Cloning and characterization of two novel DNases from Streptococcus pyogenes.

    abstract::The proteins in the culture supernatant (exoproteins) from Streptococcus pyogenes serotype M1 were separated by two-dimensional gel electrophoresis, and their N-terminal amino acid sequences were determined. The amino acid sequences were compared to sequences in the S. pyogenes genome database. The coding sequence sho...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-002-0412-8

    authors: Hasegawa T,Torii K,Hashikawa S,Iinuma Y,Ohta M

    更新日期:2002-06-01 00:00:00

  • Comparative genomic analysis of isolates belonging to the six species of the genus Thermus using pulsed-field gel electrophoresis and ribotyping.

    abstract::Fifty isolates belonging to the six validly described species of the genus Thermus (T. aquaticus, T. filiformis, T. thermophilus, T. scotoductus, T. brockianus, and T. oshimai) isolated from hot springs of different geographical areas were compared using macrorestriction analysis of genomic DNA and ribotyping. With th...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030050474

    authors: Moreira LM,Da Costa MS,Sá-Correia I

    更新日期:1997-08-01 00:00:00

  • Tetrahydrofolate serves as a methyl acceptor in the demethylation of dimethylsulfoniopropionate in cell extracts of sulfate-reducing bacteria.

    abstract::Tetrahydrofolate was shown to function as a methyl acceptor in the anaerobic demethylation of dimethylsulfoniopropionate to methylthiopropionate in cell extracts of the sulfate-reducing bacterium strain WN. Dimethylsulfoniopropionate-dependent activities were 0.56 micromol methyltetrahydrofolate min-1 (mg protein)-1 a...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030050545

    authors: Jansen M,Hansen TA

    更新日期:1998-01-01 00:00:00

  • Paenibacillus turpanensis sp. nov., isolated from a salt lake of Turpan city in Xinjiang province, north-west China.

    abstract::Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach. The optimum growth temperature was 37 °C and the optimum pH was 7.5-...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-020-02003-w

    authors: Yang L,Huang HW,Wang Y,Kou YR,Yin M,Li Y,Wang XQ,Zhao GF,Zhu WY,Tang SK

    更新日期:2021-01-01 00:00:00