Abstract:
:Polysomes were isolated both from growing gametophytes of Allomyces arbuscula and from gametangia prepared from mycelia at different periods during gametogenesis. Analysis of polysomes by sucrose gradients showed that ribosomes present in the gametangia monosome pool were shifted into polysomes. This shift was found to be correlated with gametangia differentiation. The ribosome distribution remained virtually unchanged during the early stage of gamete formation. In mature gametes and swarming zygotes a low level of polysomes was detected. Labeling of rRNA by 32PO4 demonstrated a de novo synthesis of monosomes throughout the period of gametangia differentiation. No incorporation of 32PO4 was found to be present in ribosomes prepared from gametangia after onset of gamete formation. On the basis of these labeling experiments it is concluded that radioactivity in polysomes extracted from mature gametes and swarming zygotes can be attributed in part to conserved mRNA. Synchronous formation of gametangia was induced by transferring the vegetative mycelia from growth medium into a low salt buffer. Under these conditions the incorporation of either 32PO4 or 3H-uridine into RNA, particularly into rRNA, was found to be markedly decreased. This obviously indicates a shutdown of RNA synthesis. rRNA from induced mycelia examined by polyacrylamide gel electrophoresis was found to be severely degraded. In contrast to this, rRNA isolated from ribosomes of developing gametangia and from gametes exhibited no degradation products. It is suggested that endonucleases cause rRNA hydrolysis in the hyphal cytoplasm during gametangia differentiation. Ribosomes compartmentalized in gametangia seem to be inaccessible to nucleases during the later process for gametogenesis.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Fähnrich Pdoi
10.1007/BF00413089subject
Has Abstractpub_date
1977-04-01 00:00:00pages
255-61issue
3eissn
0302-8933issn
1432-072Xjournal_volume
112pub_type
杂志文章abstract::Mesorhizobium tianshanense is a nitrogen-fixing bacterium that can establish symbiotic associations with Glycyrrhiza uralensis in the form of root nodules. Nodule formation in rhizobia often requires various secreted carbohydrates. To investigate exopolysaccharide (EPS) production and function in M. tianshanense, we p...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0345-3
更新日期:2008-05-01 00:00:00
abstract::In this study, chromium (Cr)-tolerant bacteria were test for their efficiency in alleviating Cr stress in Cicer arietinum plants. On the basis of 16S rRNA gene analysis, the isolates were identified belonging to genus Stenotrophomonas maltophilia, Bacillus thuringiensis B. cereus, and B. subtilis. The strains produced...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01801-1
更新日期:2020-05-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00417179
更新日期:1981-03-01 00:00:00
abstract::Comparative 16S rRNA gene sequence and genomic DNA reassociation analyses were used to assess the phylogenetic relationships of Methanobrevibacter fecal isolates. The 16S rRNA gene sequences of Methanobrevibacter smithii strain PS and the human fecal isolates B181 and ALI were essentially identical, and their genomic ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050589
更新日期:1998-05-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00249181
更新日期:1990-01-01 00:00:00
abstract::Aspergillus nidulans is able to grow on oleic acid as sole carbon source. Characterization of the oleate-induced beta-oxidation pathway showed the presence of the two enzyme activities involved in the first step of this catabolic system: acyl-CoA oxidase and acyl-CoA dehydrogenase. After isopicnic centrifugation in a ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050392
更新日期:1996-11-01 00:00:00
abstract::The gut microbiota inhabits the animal intestinal tract, and dysbiosis of the gut microbiota may result in disease. Senecio scandens has pharmaceutical antibacterial activities and is regarded as a broad-spectrum antibiotic in traditional Chinese medicine. Extracts of S. scandens are reported to show strong antimicrob...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02144-y
更新日期:2021-01-04 00:00:00
abstract::Stress resistance and fermentative capability are important quality characteristics of baker's yeast. In the present study, we examined protective effects of exogenous alpha-ketoglutarate (AKG), an intermediate of the tricarboxylic acid cycle and amino acid metabolism, against freeze-thaw and carbohydrate-induced stre...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-017-1423-9
更新日期:2018-01-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00249083
更新日期:1990-01-01 00:00:00
abstract::Thermotoga hypogea is an extremely thermophilic anaerobic bacterium capable of growing at 90 degrees C. It uses carbohydrates and peptides as carbon and energy sources to produce acetate, CO(2), H(2), L-alanine and ethanol as end products. Alcohol dehydrogenase activity was found to be present in the soluble fraction ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0217-x
更新日期:2007-06-01 00:00:00
abstract::The main property of an Amt- (ammonium transport negative) mutant of Klebsiella pneumoniae is its inability to accumulate NH4+ intracellularly. When growing on nitrogen sources other than NH4+, the mutant constantly looses NH3 by diffusion. This loss results in poor growth. The NH3 excretion suggests the existence of ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00402008
更新日期:1984-10-01 00:00:00
abstract::The utilization of carnitine and carnitine derivatives (O-acylcarnitines, carnitine carboxylderivatives) and structure-related trimethylammonium-compounds (betaines and nitrogen-bases) by Acinetobacter calcoaceticus was studied by means of the control of growth and the quantitative detection of metabolites. The strain...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00429336
更新日期:1977-03-01 00:00:00
abstract::The characteristics of an in vitro polyuridylic acid dependent amino acid incorporating system prepared from germinating macroconidia of Microsporum canis are described. The incorporation of 14C-phenylalanine into polyphenylalanine is dependent on S-30 extract, adenosine triphosphate, magnesium ions and polyuridylic a...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00413276
更新日期:1979-01-16 00:00:00
abstract::The synthesis of RNA was studied during the synchronous germination of Blastocladia ramosa zoospores. Comparison of RNA synthesis during germination of B. ramosa and Blastocladiella emersonii zoospores revealed that B. ramosa has a longer lag time before RNA synthesis is initiated and, in addition, the rate of RNA syn...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446529
更新日期:1975-12-31 00:00:00
abstract::A monoclonal antibody obtained by immunization of mice with heat-killed cells of Listeria monocytogenes serotype 4d showed reactivity towards a protein (P45) from L. monocytogenes with an apparent molecular mass of 45 kDa. This protein was detected in the culture supernatant and at the cell surface of L. monocytogenes...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050003
更新日期:2000-01-01 00:00:00
abstract::Fungicidic Bacillus amyloliquefaciens strains isolated from the indoor environment of moisture-damaged buildings contained heat-stable, methanol-soluble substances that inhibited motility of boar spermatozoa within 15 min of exposure and killed feline lung cells in high dilution in 1 day. Boar sperm cells lost motilit...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0660-x
更新日期:2004-04-01 00:00:00
abstract::Bacteriophages rapidly diversify their genes through co-evolution with their hosts. We hypothesize that gene diversification of phages leads to locality in phages genome. To test this hypothesis, we investigated the genetic diversity and composition of Microcystis cyanophages using 104 sequences of Ma-LMM01-type cyano...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-0980-4
更新日期:2014-06-01 00:00:00
abstract::Serine/threonine protein kinases (STPK) play a major role in the physiology and pathogenesis of Mycobacterium tuberculosis. Here, we have examined the role of pknE, a STPK in the adaptive responses of M. tuberculosis using a deletion mutant ΔpknE. The survival of ΔpknE was assessed in the presence of stress (pH, surfa...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-012-0848-4
更新日期:2013-01-01 00:00:00
abstract::Two intestinal bacterial strains MT4s-5 and MT42 involved in the degradation of (-)-epigallocatechin (EGC) were isolated from rat feces. Strain MT4s-5 was tentatively identified as Adlercreutzia equolifaciens. This strain converted EGC into not only 1-(3, 4, 5-trihydroxyphenyl)-3-(2, 4, 6-trihydroxyphenyl)propan-2-ol ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-1006-y
更新日期:2014-10-01 00:00:00
abstract::Mutants resistance to various combinations of threonine, lysine and/or their analogs were obtained and characterized in Pseudomonas acidovorans. In particular, mutants resistant to aminoethylcysteine had a dihydrodipicolinate synthetase insensitive to lysine inhibition whereas mutants resistant to threonine plus a low...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446845
更新日期:1976-03-19 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-011-0733-6
更新日期:2012-01-01 00:00:00
abstract::Exogenous addition of adenosine- and guanosine 5'-(di- and tri) phosphate 3'-diphosphate (pppApp, ppApp, pppGpp and ppGpp) at the concentration of 0.5 mM inhibits spore germination of Streptomyces galilaeus ATCC 31133. This reversible inhibitory effect appeared to be at the transcriptional level, and also depends on t...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446889
更新日期:1980-04-01 00:00:00
abstract::Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach. The optimum growth temperature was 37 °C and the optimum pH was 7.5-...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02003-w
更新日期:2021-01-01 00:00:00
abstract::The activities of NAD+-photoreduction and NADH/decyl-ubiquinone reductase in membrane preparations of Rhodobacter capsulatus changed to the same extent under different conditions. These results indicated that NADH:ubiquinone oxidoreductase (complex I) catalyzes the electron transport in the downhill direction (respira...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050548
更新日期:1998-02-01 00:00:00
abstract::A gram-negative bacterium designated BN30(T), which is motile with a polar flagellum, non-endospores forming, oxidase- and catalase-positive, was isolated from soybean root nodule. The organism is facultative anaerobic and surface-wrinkled rod. It can grow at 10-40 °C, pH 6-8 and 6 % (w/v) NaCl. BLASTn search based on...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-015-1102-7
更新日期:2015-06-01 00:00:00
abstract::The effect of intestinal molecules produced by the host on the virulence of Pseudomonas fluorescens is poorly documented. In the present work, we evaluated the secretion of human β-defensin-2 (hBD-2) by enterocytes after infection with P. fluorescens (a species previously suggested to be involved in inflammatory bowel...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-012-0865-3
更新日期:2013-03-01 00:00:00
abstract::During cultivation of a gastric fungus, Coniochaeta polymorpha, growth of Nocardia colonies on top of the fungal culture raised the question whether bacteria originated from inside of fungus. In this study, the likelihood of intracellular origin of bacteria as well as interaction of two microorganisms was assessed. Fl...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02122-4
更新日期:2021-01-02 00:00:00
abstract::Saccharomyces cerevisiaepresents two alternative vegetative forms of growth, switching between yeast forms to pseudohyphal forms depending on the specific environmental conditions. To identify genes involved in cell wall morphogenesis, a haploid S. cerevisiae monomorphic mutant, W27, which exhibits pseudohyphal growth...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0400-z
更新日期:2002-04-01 00:00:00
abstract::A new, phylogenetically distinct, dissimilatory, Fe(III)-reducing bacterium was isolated from surface sediment of a hydrocarbon-contaminated ditch. The isolate, designated strain PAL-1, was an obligately anaerobic, non-fermentative, motile, gram-negative vibrio. PAL-1 grew in a defined medium with acetate as electron ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050340
更新日期:1996-06-01 00:00:00
abstract::The regulatory elements involved in expression of the gene (fdhF) for the selenopolypeptide of formate dehydrogenase and of a gene (or transcriptional unit) (hyd) specifically responsible for the formation of the gas-evolving hydrogenase (hydrogenase 3) in Escherichia coli were investigated. Formate (or a product of i...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00429646
更新日期:1987-06-01 00:00:00