Abstract：
:Aspergillus nidulans is able to grow on oleic acid as sole carbon source. Characterization of the oleate-induced beta-oxidation pathway showed the presence of the two enzyme activities involved in the first step of this catabolic system: acyl-CoA oxidase and acyl-CoA dehydrogenase. After isopicnic centrifugation in a linear sucrose gradient, microbodies (peroxisomes) housing the beta-oxidation enzymes, isocitrate lyase and catalase were clearly resolved from the mitochondrial fraction, which contained fumarase. Growth on oleic acid was associated with the development of many microbodies that were scattered throughout the cytoplasm of the cells. These microbodies (peroxisomes) were round to elongated, made up 6% of the cytoplasmic volume, and were characterized by the presence of catalase. The beta-oxidation pathway was also induced in acetate-grown cells, although at lower levels; these cells lacked acyl-CoA oxidase activity. Nevertheless, growth on acetate did not cause a massive proliferation of microbodies in A. nidulans.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Valenciano S,Lucas JR,Pedregosa A,Monistrol IF,Laborda Fdoi
10.1007/s002030050392subject
Has Abstractpub_date
1996-11-01 00:00:00pages
336-41issue
5eissn
0302-8933issn
1432-072Xjournal_volume
166pub_type
杂志文章abstract：:We cloned the Kluyveromyces lactis KlNTH1 gene, which encodes neutral trehalase. It showed 65.2% and 68.5% identity at nucleotide and amino acid sequence level, respectively, with the Saccharomyces cerevisiae NTH1 gene. Multiple alignment of the predicted trehalase protein sequences from yeasts, bacteria, insects, and...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050436
更新日期：1997-04-01 00:00:00
abstract：:Mannoproteins from cell walls of Saccharomyces cerevisiae synthesized at successive stages of the population growth cycle have been solubilized with Zymolyase and subsequently analyzed. The major change along the population cycle concerned a large size mannoprotein material; the size of the newly-synthesized molecules...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425354
更新日期：1987-07-01 00:00:00
abstract：:The major cobamide-containing protein from methanol-utilizing Sporomusa ovata was 8-fold enriched to apparent homogeneity. The protein exhibited a molecular mass of 40 kDa and of 38 kDa determined by gel filtration and by SDS-polyacrylamide gel electrophoresis, respectively. This finding indicates a monomeric protein ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245367
更新日期：1992-01-01 00:00:00
abstract：:The mitochondrial genome (mtDNA) of the entomopathogenic fungus Metarhizium anisopliae var. anisopliae, with a total size of 24,673 bp, was one of the smallest known mtDNAs of Pezizomycotina. It contained the 14 typical genes coding for proteins related to oxidative phosphorylation, the two rRNA genes, a single intron...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0104-x
更新日期：2006-06-01 00:00:00
abstract：:The halophilic archaeal strain GX71(T) was isolated from the Gangxi marine solar saltern near the Weihai city of Shandong Province, China. Cells of the strain were pleomorphic and lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. Strain GX71(T) was able to grow at 25-45 °C (optimum 30 ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-0975-1
更新日期：2014-06-01 00:00:00
abstract：:Partitioning in aqueous polymer two-phase systems of polyethylene glycol and dextran was used to detect and compare cell-surface charge and cell-surface hydrophobicity of Aeromonas hydrophila, A. caviae, A. sobria, Vibrio cholerae, and V. anguillarum strains. These strains have cell-surface components that bound eithe...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF02529975
更新日期：1995-09-01 00:00:00
abstract：:Cell free extracts prepared from exponentially growing Escherichia coli and Bacillus cereus as well as from Bacillus cereus at the end of exponential growth were optimized for various factors required for amino acid incorporation when programmed with Bacillus ribonucleic acid. All three preparations synthesized glutam...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00455354
更新日期：1981-04-01 00:00:00
abstract：:Microbial flavohaemoglobins are proteins with homology to haemoglobins from higher organisms, but clearly linked to nitric oxide (NO) metabolism by bacteria and yeast. hmp mutant strains of several bacteria are hypersensitive to NO and related compounds and hmp genes are up-regulated by the presence of NO. The regulat...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0175-8
更新日期：2007-01-01 00:00:00
abstract：:A bacterium capable of using the carbamate insecticide carbofuran as a sole source of carbon and energy, was isolated from soil. The ability to catabolise carbofuran phenol, produced by cleavage of the carbamate ester linkage of the insecticide, was lost at very high frequency when the bacterium was grown in the absen...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245249
更新日期：1992-01-01 00:00:00
abstract：:The rubredoxin content of Acinetobacter calcoaceticus in dependence on the carbon source (acetate, n-alkanes, succinate, L-malate) and on the growth phase was studied by means of a radioimmunoassay. The method used was specific for rubredoxin and Acinetobacter calcoaceticus. The formation of rubredoxin increased with ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406170
更新日期：1980-12-01 00:00:00
abstract：:The time dependent assembly of the photosynthetic apparatus was studied in Rhodospirillum rubrum after transfer of cells growing aerobically in the dark to low aeration. While bacteriochlorophyll (Bchl) cellular levels increase continuously levels of soluble cytochrome C2 do not change significantly. Absorption spectr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454853
更新日期：1976-07-01 00:00:00
abstract：:The regulatory elements involved in expression of the gene (fdhF) for the selenopolypeptide of formate dehydrogenase and of a gene (or transcriptional unit) (hyd) specifically responsible for the formation of the gas-evolving hydrogenase (hydrogenase 3) in Escherichia coli were investigated. Formate (or a product of i...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429646
更新日期：1987-06-01 00:00:00
abstract：:A fromaldehyde oxidase activity was found in cellfree extracts of methanol-grown yeast Candida boidinii. Loss of alcohol oxidase activity in a mutant, 48, led to loss of the formaldehyde oxidase activity, indicating that the same enzyme is probably responsible for both activities. This could be demonstrated with the p...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447134
更新日期：1975-10-27 00:00:00
abstract：:Cyanothece sp. strain ATCC 51142 is an aerobic, unicellular, diazotrophic cyanobacterium that temporally separates O2-sensitive N2 fixation from oxygenic photosynthesis. The energy and reducing power needed for N2 fixation appears to be generated by an active respiratory apparatus that utilizes the contents of large i...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:Pseudomonas cepacia grew rapidly using a mixture of all three branched chain amino acids as carbon source, but failed to use individual branched chain amino acids as sole carbon source. Extracts of bacteria grown on branched chain amino acids had between 2- and 3-fold higher levels of alpha-ketoglutarate-dependent bra...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00423069
更新日期：1979-03-12 00:00:00
abstract：:Cadmium is a widespread pollutant that has been associated with oxidative stress, but the mechanism behind this effect in prokaryotes is still unclear. In this work, we exposed two glutathione deficient mutants (DeltagshA and DeltagshB) and one respiration deficient mutant (DeltaubiE) to a sublethal concentration of c...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0316-8
更新日期：2008-03-01 00:00:00
abstract：:Virulence factors of H. pylori, such as outer inflammatory protein A (oipA), are closely involved in the development of gastric diseases such as chronic gastritis and gastric cancer. The functional status of oipA is regulated by a repair mechanism based on CT dinucleotide repeats that influence the reading frame, thus...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-018-1580-5
更新日期：2019-01-01 00:00:00
abstract：:When Clostridium formicoaceticum was grown on fumarate or L-malate crude cell extracts contained a high fumarate reductase activity. Using reduced methyl viologen as electron donor the specific activity amounted to 2-3.5 U per mg of protein. Reduced benzyl viologen, FMNH2 and NADH could also serve as electron donors b...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00407920
更新日期：1978-10-04 00:00:00
abstract：:Bacillus cereus ATCC 14579 transformed naphthalene predominately to 1-naphthol. Experiments with [14C]naphthalene showed that over a 24 h period, B. cereus oxidized 5.2% of the added naphthalene. 1-Naphthol accounted for approximately 80% of the total metabolites. B. cereus incubated with naphthalene under the presenc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00410891
更新日期：1984-08-01 00:00:00
abstract：:A gram-negative bacterium designated BN30(T), which is motile with a polar flagellum, non-endospores forming, oxidase- and catalase-positive, was isolated from soybean root nodule. The organism is facultative anaerobic and surface-wrinkled rod. It can grow at 10-40 °C, pH 6-8 and 6 % (w/v) NaCl. BLASTn search based on...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1102-7
更新日期：2015-06-01 00:00:00
abstract：:Coagulase-negative staphylococci (CNS) are opportunistic pathogens that are currently emerging as causative agents of human disease. Though CNS are widespread in the clinic and food, their precise identification at species level is important. Here, using 16S rRNA sequencing, 55 staphylococcal isolates were identified ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1415-9
更新日期：2018-01-01 00:00:00
abstract：:Mycothiol (MSH) plays important roles in maintaining cytosolic redox homeostasis and in adapting to reactive oxygen species in the high-(G + C)-content Gram-positive Actinobacteria. However, its physiological roles are ill defined compared to glutathione, the functional analog of MSH in Gram-negative bacteria and most...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0889-3
更新日期：2013-06-01 00:00:00
abstract：:During the course of isolating rare actinobacteria from unexplored habitats, strain CH32T was obtained from an arid soil sample in eastern Anatolia, Turkey. Polyphasic characterization and comprehensive genome analyses showed that the strain is a member of the genus Nonomuraea and it is closely related to Nonomuraea g...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01941-9
更新日期：2020-10-01 00:00:00
abstract：:Lactic acid bacteria can be considered as natural biopreservative and good biotechnological alternative to food safety. In this study, the antilisterial compounds produced by Enterococcus isolates from the Patagonian environment and their effectiveness for the control of Listeria monocytogenes in a food model were stu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02118-0
更新日期：2021-01-02 00:00:00
abstract：:The presence of teichoic acids in a number of streptomycetes led to the conclusion that these biopolymers were widely spread in genus Streptomyces. The nature of the teichoic acid present in the mycelium was determined by extracting it with 10% trichloroacetic acid, precipitating it with ethanol and identifying the pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00421893
更新日期：1980-05-01 00:00:00
abstract：:1. An already published test method for detecting bactericidal substances in paper chromatograms was further improved. 2. In cultures of Cosmarium impressulum free from bacteria, two bactericidal substances were found in the ether extracts from the algae and two others in the extracts of the culture medium. The are ac...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428945
更新日期：1976-06-01 00:00:00
abstract：:Chemoautotrophic endosymbionts residing in Solemya velum gills provide this shallow water clam with most of its nutritional requirements. The cbb gene cluster of the S. velum symbiont, including cbbL and cbbS, which encode the large and small subunits of the carbon-fixing enzyme ribulose 1,5-bisphosphate carboxylase/o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0689-x
更新日期：2004-09-01 00:00:00
abstract：:Stress resistance and fermentative capability are important quality characteristics of baker's yeast. In the present study, we examined protective effects of exogenous alpha-ketoglutarate (AKG), an intermediate of the tricarboxylic acid cycle and amino acid metabolism, against freeze-thaw and carbohydrate-induced stre...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1423-9
更新日期：2018-01-01 00:00:00
abstract：:Respiratory NADH oxidation in the rumen bacterium Prevotella bryantii is catalyzed by the Na+-translocating NADH:quinone oxidoreductase (NQR). A method for cell disruption and membrane isolation of P. bryantii under anoxic conditions using the EmulisFlex-C3 homogenizer is described. We compared NQR activity and protei...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01805-x
更新日期：2020-07-01 00:00:00
abstract：:Streptococcus intermedius is a causative agent of brain or liver abscesses. S. intermedius produces intermedilysin that plays a pivotal role in pathogenicity. We identified other pathogenic factors and described a fibronectin binding protein (FBP) homolog of S. intermedius (FbpI) that mediated bacterial adhesion to ep...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01922-y
更新日期：2020-10-01 00:00:00
