Abstract:
:Mannoproteins from cell walls of Saccharomyces cerevisiae synthesized at successive stages of the population growth cycle have been solubilized with Zymolyase and subsequently analyzed. The major change along the population cycle concerned a large size mannoprotein material; the size of the newly-synthesized molecules varied from 120,000-500,000 (mean of about 200,000) at early exponential phase to 250,000-350,000 (mean of about 300,000) at late exponential phase. These differences are due to modifications in the amount of N-glycosidically linked mannose residues, since the size of the peptide moiety was 90,000-100,000 at all growth stages and the level of O-glycosylation changed only slightly. After incubation of the purified walls with concanavalin A-ferritin and subsequent analysis by electron microscopy, labelling was localized at the external and internal faces of the walls. The middle space of these was labelled after digestion of the glucan network with Zymolyase, which demonstrate the presence of mannoproteins in close contact with the structural glucan molecules throughout the wall.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Valentín E,Herrero E,Rico H,Miragall F,Sentandreu Rdoi
10.1007/BF00425354subject
Has Abstractpub_date
1987-07-01 00:00:00pages
88-94issue
2eissn
0302-8933issn
1432-072Xjournal_volume
148pub_type
杂志文章abstract::We investigated the structural and immunochemical characteristics of cell wall mannan obtained from Candida sojae JCM 1644, which is a new yeast species isolated from defatted soybean flakes. The results of a slide-agglutination test and of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic ...
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journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:1977-10-24 00:00:00
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journal_title:Archives of microbiology
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更新日期:1978-10-04 00:00:00
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abstract::An Arthrobacter sp. growing on 4-Chlorobenzoic acid as its sole source of carbon excretes 4-hydroxygenzoic acid and protocatechuic acid into the culture medium. Protocatechuic acid is further attacked by "meta"-cleavage. During growth of the Arthrobacter sp. on benzoic acid cis-cis muconic acid can be isolated from th...
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更新日期:1989-01-01 00:00:00
abstract::1. The daily progress of NADP-linked GPD-activity rise in synchronous culture of Ankistrodesmus braunii was investigated in respect to short time increase of activity by light. After various exposure times cells were temporarily deprived of light and subsequently the so-called dark value as well as the light value (da...
journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:1975-06-22 00:00:00
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journal_title:Archives of microbiology
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更新日期:2013-03-01 00:00:00
abstract::Two different acidic polysaccharides (I and II) were detected in the external cell envelope layers (slime and capsule) of Rhodopseudomonas capsulata Sp11. Polysaccharide I contains rhamnose, fucose, glucosamine and an unknown acidic sugar, it represents the slime material of the strain. Polysaccharide II contains rham...
journal_title:Archives of microbiology
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abstract::The thermotolerant methylotroph Bacillus sp. C1 possesses a novel NAD-dependent methanol dehydrogenase (MDH), with distinct structural and mechanistic properties. During growth on methanol and ethanol, MDH was responsible for the oxidation of both these substrates. MDH activity in cells grown on methanol or glucose wa...
journal_title:Archives of microbiology
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更新日期:2011-05-01 00:00:00
abstract::Pyruvate kinase (ATP: pyruvate phosphotransferase (EC 2.7.1.40) was partially purified from both autotrophically and heterotrophically grown Paracoccus denitrificans. The organism grown under heterotrophic conditions contains four times more pyruvate kinase than under autotrophic conditions. The enzyme isolated from b...
journal_title:Archives of microbiology
pub_type: 杂志文章
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abstract::Succinate:quinone reductase is a membrane-bound enzyme of the citric acid cycle and the respiratory chain. Carboxin is a potent inhibitor of the enzyme of certain organisms. The bacterium Paracoccus denitrificans was found to be sensitive to carboxin in vivo, and mutants that grow in the presence of 3'-methyl carboxin...
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