Bradyrhizobium campsiandrae sp. nov., a nitrogen-fixing bacterial strain isolated from a native leguminous tree from the Amazon adapted to flooded conditions.

Abstract:

:The nitrogen-fixing bacterial strain UFLA 01-1174T was isolated from nodules of Campsiandra laurilifolia Benth. originating from the Amazon region, Brazil. Its taxonomic position was defined using a polyphasic approach. Analysis of the 16S rRNA gene placed the strain in the Bradyrhizobium genus, the closest species being B. guangdongense CCBAU 51649T and B. guangzhouense CCBAU 51670T, both with 99.8% similarity. Multilocus sequence analysis (MLSA) of recA, gyrB, glnII, rpoB, atpD, and dnaK indicated that UFLA 01-1174T is a new species, most closely related to B. stylosanthis BR 446T (94.4%) and B. manausense BR 3351T (93.7%). Average nucleotide identity (ANI) differentiated UFLA 01-1174T from the closest species with values lower than 90%. The G + C content in the DNA of UFLA 01-1174T is 63.6 mol%. Based on this data, we conclude that the strain represents a new species. The name proposed is Bradyrhizobium campsiandrae, with UFLA 01-1174T (= INPA 394BT = LMG 10099T) as type strain.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Cabral Michel D,Martins da Costa E,Azarias Guimarães A,Soares de Carvalho T,Santos de Castro Caputo P,Willems A,de Souza Moreira FM

doi

10.1007/s00203-020-02022-7

subject

Has Abstract

pub_date

2021-01-01 00:00:00

pages

233-240

issue

1

eissn

0302-8933

issn

1432-072X

pii

10.1007/s00203-020-02022-7

journal_volume

203

pub_type

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