Abstract:
:Effects of different flavonoids on various bacterial strains have been extensively reported; however, the mechanism(s) of their action on bacterial cells remain largely elusive. In this study, the antibacterial mechanism of soybean isoflavone (SI) on Staphylococcus aureus is systematically investigated using 4'6-diamidino-2-phenylindole (DAPI) staining, pBR322DNA decatenation experiment mediated by topoisomerase and agarose gel electrophoresis for direct decatenation. The results of fluorescence microscopy and fluorescence spectrophotometer indicated that DAPI was integrated in Staphylococcus aureus. Additionally, the quantity of both DNA and RNA reduced to 66.47 and 60.18%, respectively, after treated with SI for 28 h. Effects of SI on topoisomerase I and II were also investigated. SI completely inhibited the pBR322DNA unwinding mediated by topoisomerase I and topoisomerase II at the concentration of 6.4 mg/ml and could denature the plasmid DNA at the concentration of 12.8 mg/ml. These results indicate that topoisomerase I and II are the most important targets by SI to restrain bacterial cell division.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Wang Q,Wang H,Xie Mdoi
10.1007/s00203-010-0617-1subject
Has Abstractpub_date
2010-11-01 00:00:00pages
893-8issue
11eissn
0302-8933issn
1432-072Xjournal_volume
192pub_type
杂志文章abstract::Acyl carrier protein (ACP) is a small acidic protein that acts as an essential cofactor in many biosynthetic pathways depending on acyl transfer reactions. In this work, a Vibrio anguillarum ACP encoding gene, acpV, was first cloned from the chromosome of a virulent V. anguillarum strain MVM425. acpV was over-expresse...
journal_title:Archives of microbiology
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journal_title:Archives of microbiology
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doi:10.1007/s00203-012-0793-2
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journal_title:Archives of microbiology
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doi:10.1007/BF00409659
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pub_type: 杂志文章
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425338
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journal_title:Archives of microbiology
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journal_title:Archives of microbiology
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doi:10.1007/BF00436334
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050568
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journal_title:Archives of microbiology
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doi:10.1007/s00203-006-0121-9
更新日期:2006-07-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00245305
更新日期:1993-01-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00248480
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00258144
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0492-5
更新日期:2002-12-01 00:00:00
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journal_title:Archives of microbiology
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更新日期:2005-03-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00416596
更新日期:1989-01-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00402336
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journal_title:Archives of microbiology
pub_type: 杂志文章
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journal_title:Archives of microbiology
pub_type: 杂志文章
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-006-0104-x
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-017-1350-9
更新日期:2017-07-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00429646
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journal_title:Archives of microbiology
pub_type: 杂志文章
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journal_title:Archives of microbiology
pub_type: 杂志文章
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00407766
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00288712
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journal_title:Archives of microbiology
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更新日期:2014-04-01 00:00:00