Abstract：
:The regulatory elements involved in expression of the gene (fdhF) for the selenopolypeptide of formate dehydrogenase and of a gene (or transcriptional unit) (hyd) specifically responsible for the formation of the gas-evolving hydrogenase (hydrogenase 3) in Escherichia coli were investigated. Formate (or a product of it) is required for expression of both systems since in a pyruvate-formate-lyase deficient mutant induction occurs only when formate is supplemented externally. Under this condition, formate can partially overcome repression by nitrate. The transcription of both the fdhF gene and the hydrogenase-3-encoding systems is independent of the presence of a wild-type fnr gene when formate is present, supporting the view that the Fnr effect on the formation of the formate-hydrogen-lyase pathway is indirect. Mutations blocking the synthesis of a functional molybdenum cofactor also had no major affect on fdhF and hyd expression. The nucleotide sequence of the 5' flanking region of the fdhF gene was determined and the transcription start point of the fdhF gene was localized by nuclease S1 mapping. Nuclease Bal31 generated deletion clones were constructed and the regulation of their expression was studied. Anaerobic expression and induction by formate depended on the presence of a stretch of approximately 185 nucleotides upstream of the translation start. Elements mediating formate induction and oxygen or nitrate repression could not be separated physically. The regulatory features of the fdhF upstream region bear striking resemblance to systems whose expression are dependent upon upstream activating elements.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Birkmann A,Zinoni F,Sawers G,Böck Adoi
10.1007/BF00429646subject
Has Abstractpub_date
1987-06-01 00:00:00pages
44-51issue
1eissn
0302-8933issn
1432-072Xjournal_volume
148pub_type
杂志文章abstract：:Cyanothece sp. strain ATCC 51142 is an aerobic, unicellular, diazotrophic cyanobacterium that temporally separates O2-sensitive N2 fixation from oxygenic photosynthesis. The energy and reducing power needed for N2 fixation appears to be generated by an active respiratory apparatus that utilizes the contents of large i...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:Pure cultures of an aerobically grown Flavobacterium sp. were shown by hydride generation-cold trap-atomic absorption spectrometry to biomethylate inorganic antimony (III) supplied as potassium antimony tartrate. Growth inhibition of the Flavobacterium sp. by antimony (III) over the range 0-30 mg Sb l(-1) was assessed...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0456-9
更新日期：2002-10-01 00:00:00
abstract：:Carbon-14 was incorporated into oxalate and CO2 from either citrate-1,5-14C, succinate-1,4-14C, or fumarate-1,4-14C by cultures of Aspergillus niger pregrown on a medium which contained glucose as the sole carbon source and which did not allow citrate accumulation. In cell-free extracts of mycelium forming oxalate and...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447318
更新日期：1975-06-22 00:00:00
abstract：:The TTA codon, one of the six available codons for the amino acid leucine, is the rarest codon among the high GC genomes of Actinobacteria including Frankia. This codon has been implicated in various regulatory mechanisms involving secondary metabolism and morphological development. TTA-mediated gene regulation is wel...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0731-8
更新日期：2012-01-01 00:00:00
abstract：:A gram-negative bacterium designated BN30(T), which is motile with a polar flagellum, non-endospores forming, oxidase- and catalase-positive, was isolated from soybean root nodule. The organism is facultative anaerobic and surface-wrinkled rod. It can grow at 10-40 °C, pH 6-8 and 6 % (w/v) NaCl. BLASTn search based on...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1102-7
更新日期：2015-06-01 00:00:00
abstract：:The upstream region of the isocitrate lyase gene (UPR-ICL, 1530bp) of an n-alkane-utilizable yeast, Candida tropicalis, induced gene expression in another yeast, Saccharomyces cerevisiae, when the yeasts were grown on acetate. Surprisingly, UPR-ICL displayed the same regulatory function in the bacterium Escherichia co...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00404204
更新日期：1995-05-01 00:00:00
abstract：:The utilization of carnitine and carnitine derivatives (O-acylcarnitines, carnitine carboxylderivatives) and structure-related trimethylammonium-compounds (betaines and nitrogen-bases) by Acinetobacter calcoaceticus was studied by means of the control of growth and the quantitative detection of metabolites. The strain...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429336
更新日期：1977-03-01 00:00:00
abstract：:The proteins in the culture supernatant (exoproteins) from Streptococcus pyogenes serotype M1 were separated by two-dimensional gel electrophoresis, and their N-terminal amino acid sequences were determined. The amino acid sequences were compared to sequences in the S. pyogenes genome database. The coding sequence sho...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0412-8
更新日期：2002-06-01 00:00:00
abstract：:The activities of NAD+-photoreduction and NADH/decyl-ubiquinone reductase in membrane preparations of Rhodobacter capsulatus changed to the same extent under different conditions. These results indicated that NADH:ubiquinone oxidoreductase (complex I) catalyzes the electron transport in the downhill direction (respira...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050548
更新日期：1998-02-01 00:00:00
abstract：:Exoglucanase (exo-1,3-beta-D-glucan glycohydrolase, EC 3.2.1.56) activity secreted by Saccharomyces cerevisiae into the culture medium was separated by ion exchange chromatography into two glycoprotein isoenzymes which contributed 10% (exoglucanase I) and 90% (exoglucanase II) towards the total activity. Analysis of t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00416596
更新日期：1989-01-01 00:00:00
abstract：:In order to obtain strains of Saccharomycopsis lipolytica impaired in the active transport of L-lysine, mutants resistant to a mixture of L-canavanine, L-4-5-transdehydrolysine and L-S-amino ethylcysteine, taken either all three or two by two, were isolated. These compounds were shown previously to be competitive inhi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00411361
更新日期：1979-08-06 00:00:00
abstract：:Bacteriochlorophyll (BChl) c in whole cells of Chlorobium tepidum grown at 46 degrees C changed into bacteriopheophytin (BPhe) c within 10 days after reaching full growth. When a small amount of C. tepidum cells in which BChl c had been completely pheophytinized were transferred to a new culture medium, normal growth ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050737
更新日期：1999-07-01 00:00:00
abstract：:Maize is highly responsive to the application of nitrogen to achieve high productivity. Inoculation with diazotrophic bacteria can improve plant growth with low N fertilization. The objective was to evaluate the inoculation of two species of diazotrophs on N metabolism in maize plants, in the presence of two concentra...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-018-1594-z
更新日期：2019-05-01 00:00:00
abstract：:The applicability of flow-microfluorometer to separate microbial cells was demonstrated with algal and bacterial cells. Algal mixtures were sorted according to the natural chlorophyll fluorescence and the bacterial mixtures were sorted according to the fluorescence of ethidium bromide-stained nucleic acid. ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00423075
更新日期：1979-03-12 00:00:00
abstract：:The bacteriocinogenic strain of Enterococcus faecalis EJ97 has been isolated from municipal waste water. It produces a cationic bacteriocin (enterocin EJ97) of low molecular mass (5,340 Da) that is very stable under mild heat conditions and is sensitive to proteolytic enzymes. The amino acid sequence of the first 18 N...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050678
更新日期：1998-12-01 00:00:00
abstract：:The development of peritrichous flagella and, consequently, swarming of Vibrio alginolyticus depend on a complex relationship between temperature, salt concentrations and pH. At temperatures above 28 degrees C V. alginolyticus did not develop peritrichous flagella unless certain minimal concentrations of NaCl are pres...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447338
更新日期：1975-08-28 00:00:00
abstract：:A strictly anaerobic bacterium, strain PCE1, was isolated from a tetrachloroethene-dechlorinating enrichment culture. Cells of the bacterium were motile curved rods, with approximately four lateral flagella. They possessed a gram-positive type of cell wall and contained cytochrome c. Optimum growth occurred at pH 7.2-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050308
更新日期：1996-02-01 00:00:00
abstract：:An alkaliphilic actinobacterial strain, designated Hz 6-5T, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China. The isolate formed light yellow-colored colonies and its cells were Gram-staining positive, non-motile, and non-spore-forming short rods. The strain was aerobic with optimal gro...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02073-w
更新日期：2020-10-26 00:00:00
abstract：:Non-hemolytic enterotoxin (NHE), a tri-partite, proteinaceous toxin encoded by contiguous nheA, nheB and nheC genes of Bacillus cereus sensu lato (B. cereus s.l.), is considered to be associated with the foodborne diarrheic syndrome. However, B. cereus s.l. includes a number of closely related strains, and the occurre...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1350-9
更新日期：2017-07-01 00:00:00
abstract：:Succinate:quinone reductase is a membrane-bound enzyme of the citric acid cycle and the respiratory chain. Carboxin is a potent inhibitor of the enzyme of certain organisms. The bacterium Paracoccus denitrificans was found to be sensitive to carboxin in vivo, and mutants that grow in the presence of 3'-methyl carboxin...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050611
更新日期：1998-07-01 00:00:00
abstract：:Penicillium marneffei is a dimorphic fungus that intracellularly infects the reticuloendothelial system of humans and bamboo rats. Endemic in Southeast Asia, it infects 10% of AIDS patients in this region. The absence of a sexual stage and the highly infectious nature of the mould-phase conidia have impaired studies o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0533-8
更新日期：2003-05-01 00:00:00
abstract：:Cadmium is a non-essential toxic heavy metal for organisms, including plants and cyanobacteria. Cadmium resistance transporters involved in resistance of cells against various toxicants such as drugs and effluxes cytotoxic compounds from cells. However, cadmium resistance-associated protein (CadD) has never been repor...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1462-2
更新日期：2018-04-01 00:00:00
abstract：:1. With fumarate as the terminal electron acceptor and either H2 or formate as donor, Vibrio succinogenes could grow anaerobically in a mineral medium using fumarate as the sole carbon source. Both the growth rate and the cell yield were increased when glutamate was also present in the medium. 2. Glutamate was incorpo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00405882
更新日期：1982-05-01 00:00:00
abstract：:Twelve genes involved in thiamin biosynthesis in prokaryotes have been identified and overexpressed. Of these, six are required for the thiazole biosynthesis (thiFSGH, thil, and dxs), one is involved in the pyrimidine biosynthesis (thiC), one is required for the linking of the thiazole and the pyrimidine (thiE), and f...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s002030050713
更新日期：1999-04-01 00:00:00
abstract：:The ability of the imperfect soil fungus Paecilomyces lilacinus to transform the environmental pollutant dibenzofuran was investigated. Transformation of dibenzofuran and related derivatives lead to 14 products, which were identified by UV spectroscopy, mass spectrometry, and proton nuclear magnetic resonance spectros...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0695-z
更新日期：2004-09-01 00:00:00
abstract：:Two different acidic polysaccharides (I and II) were detected in the external cell envelope layers (slime and capsule) of Rhodopseudomonas capsulata Sp11. Polysaccharide I contains rhamnose, fucose, glucosamine and an unknown acidic sugar, it represents the slime material of the strain. Polysaccharide II contains rham...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425219
更新日期：1983-12-01 00:00:00
abstract：:Both the changes in the activities of nitrogenase, glutamine synthetase and glutamate dehydrogenase and in the extracellular and intracellular NH4+ concentrations were investigated during the transition from an NH4+ free medium to one containing NH4+ ions for a continuous culture of Azotobacter vinelandii. If added in...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447319
更新日期：1975-06-22 00:00:00
abstract：:The Gram-positive bacterium Clavibacter michiganensis subsp. sepedonicus is the causal agent of bacterial wilt and ring rot of potato. So far, only two proteins have been shown to be essential for virulence, namely a plasmid-encoded cellulase CelA and a hypersensitive response-inducing protein. We have examined the re...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0301-2
更新日期：2008-02-01 00:00:00
abstract：:Geobacter species such as G. bremensis, G. pelophilus, and G. sulfurreducens are obligately anaerobic and grow in anoxic, non-reduced medium by fast reduction of soluble ferric citrate. In contrast, insoluble ferrihydrite was either only slowly or not reduced when supplied as electron acceptor in similar growth experi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0686-0
更新日期：2004-10-01 00:00:00
abstract：:Many bacterial cells are known to regulate their cooperative behaviors and physiological processes through a molecular mechanism called quorum sensing. Quorum sensing in Serratia marcescens JG is mediated by the synthesis of autoinducer 2 (AI-2) which is a furanosyl borate diester. In this study, the response of quoru...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1213-9
更新日期：2016-08-01 00:00:00
